Expression of NR3C1, INSR and SLC2A4 genes in skeletal muscles and CBG in liver depends on age and breed of pigs D., Skrzypski M., Pruszyńska-Oszmałek E., Sadkowski S., Sassek M., Wojciechowicz T., Kołodziejski P.A., Kaczmarek P., Szydłowski M., Maćkowiak P., Nowak K.W. (2019): Expression of NR3C1, INSR and SLC2A4 genes in skeletal muscles and CBG in liver depends on age and breed of pigs. Czech J. Anim. Sci., 64: 343-351.
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The genes encoding glucose transporter 4 (SLC2A4, GLUT4), insulin receptor (INSR) and glucocorticoid receptor (NR3C1) are considered regulators of energy metabolism that may influence fat content in skeletal muscle at different ages and breeds of pigs. In the study we performed analysis of expression of NR3C1, INSR and SLC2A4 genes in two skeletal muscle tissues: semimembranosus and longissimus dorsi muscle in gilts from three breeds of pigs that differed in intramuscular fat content: Duroc (DU), Pulawska (PUL) and Polish Large White (PLW) at 60, 90, 120, 150, 180 and 210 days after weaning. We also analyzed the expression of cortisol binding globulin (CBG) in the liver. Expression was analyzed with real time PCR (qPCR) using a relative quantification method. In blood, the concentration of cortisol, insulin and leptin were evaluated with radioimmunoassay. The concentration of metabolites (triglycerides and glucose) related to the changes of lipids content was also measured. We observed a significant relationship between the breed and the age of pigs and the expression of analyzed genes in muscle tissues (P < 0.01 for all analyzed genes) as well as with two hormones (for insulin P = 0.001; for cortisol P < 0.0001). Leptin level and metabolites in serum were not significant in the model (P > 0.05). The expression of analyzed genes (SLC2A4, INSR and NR3C1 in muscles and CBG in liver) strongly correlates with intramuscular fat content in pigs and is associated with age and breed.

Bosch L., Tor M., Reixach J., Estany J. (2012): Age-related changes in intramuscular and subcutaneous fat content and fatty acid composition in growing pigs using longitudinal data. Meat Science, 91, 358-363
Bright G.M. (1995): Corticosteroid-binding globulin influences kinetic parameters of plasma cortisol transport and clearance. Journal of Clinical Endocrinology Metabolism, 80, 770–775.
Casellas J., Vidal O., Pena R. N., Gallardo D., Manunza A., Quintanilla R., Amills M. (2013): Genetics of serum and muscle lipids in pigs. Animal Genetics, 44, 609-619
Geverink N. A., Foury A., Plastow G. S., Gil M., Gispert M., Hortós M., Furnols M. Font i, Gort G., Moisan M. P., Mormède P. (2006): Cortisol-binding globulin and meat quality in five European lines of pigs1. Journal of Animal Science, 84, 204-211
Giorgino F, Almahfouz A, Goodyear L J, Smith R J (1993): Glucocorticoid regulation of insulin receptor and substrate IRS-1 tyrosine phosphorylation in rat skeletal muscle in vivo.. Journal of Clinical Investigation, 91, 2020-2030
Glass Karen B., Czikk Marie J. (2005): Diagnosing insulin resistance—a clarification. Fertility and Sterility, 83, 521-522
Hafizi Abu Bakar Mohamad, Kian Kai Cheng, Wan Hassan Wan Najihah, Sarmidi Mohamad Roji, Yaakob Harisun, Zaman Huri Hasniza (2015): Mitochondrial dysfunction as a central event for mechanisms underlying insulin resistance: the roles of long chain fatty acids. Diabetes/Metabolism Research and Reviews, 31, 453-475
Hou June Chunqiu, Pessin Jeffrey E (2007): Ins (endocytosis) and outs (exocytosis) of GLUT4 trafficking. Current Opinion in Cell Biology, 19, 466-473
KATSUMATA Masaya (2011): Promotion of intramuscular fat accumulation in porcine muscle by nutritional regulation. Animal Science Journal, 82, 17-25
Kouba Maryline, Sellier Pierre (2011): A review of the factors influencing the development of intermuscular adipose tissue in the growing pig. Meat Science, 88, 213-220
Larsen M.O., Rolin B., Wilken M., Carr R.D., Svendsen O., Bollen P. (2001): Parameters of glucose and lipid metabolism in the male Göttingen minipig: Influence of age, body weight, and breeding family. Comparative Medicine, 51, 436–442.
Liang Y., Yang X.M., Gu Y.R., Tao X., Zhong Z.Z., Gong J.J., Chen X.H., Lv X.B. (2015): Developmental changes in the expression of the GLUT2 and GLUT4 genes in the longissimus dorsi muscle of Yorkshire and Tibetan pigs. Genetics and Molecular Research, 14, 1287-1292
Matsakas A., Patel K. (2009): Skeletal muscle fibre plasticity in response to selected environmental and physiological stimuli. Histology and Histopathology, 24, 611–629.
Ousova Olga, Guyonnet-Duperat Véronique, Iannuccelli Nathalie, Bidanel Jean-Pierre, Milan Denis, Genêt Carine, Llamas Bastien, Yerle Martine, Gellin Joël, Chardon Patrick, Emptoz-Bonneton Agnès, Pugeat Michel, Mormède Pierre, Moisan Marie-Pierre (2004): Corticosteroid Binding Globulin: A New Target for Cortisol-Driven Obesity. Molecular Endocrinology, 18, 1687-1696
Quintanilla R., Pena R. N., Gallardo D., Cánovas A., Ramírez O., Díaz I., Noguera J. L., Amills M. (2011): Porcine intramuscular fat content and composition are regulated by quantitative trait loci with muscle-specific effects1. Journal of Animal Science, 89, 2963-2971
Saltiel Alan R, Pessin Jeffrey E (2002): Insulin signaling pathways in time and space. Trends in Cell Biology, 12, 65-71
SMITH CAROLYN L., HAMMOND GEOFFREY L. (1991): Ontogeny of Corticosteroid-Binding Globulin Biosynthesis in the Rat*. Endocrinology, 128, 983-988
Tyra M., Ropka-Molik K., Eckert R., Piórkowska K., Oczkowicz M. (2011): H-FABP and LEPR gene expression profile in skeletal muscles and liver during ontogenesis in various breeds of pigs. Domestic Animal Endocrinology, 40, 147-154
Tyra M., Ropka-Molik K., Terman A., Piórkowska K., Oczkowicz M., Bereta A. (2013): Association between subcutaneous and intramuscular fat content in porcine ham and loin depending on age, breed and FABP3 and LEPR genes transcript abundance. Molecular Biology Reports, 40, 2301-2308
Vegiopoulos Alexandros, Herzig Stephan (2007): Glucocorticoids, metabolism and metabolic diseases. Molecular and Cellular Endocrinology, 275, 43-61
VESTERGAARD Henrik, BRATHOLM Palle, CHRISTENSEN Niels Juel (2001): Increments in insulin sensitivity during intensive treatment are closely correlated with decrements in glucocorticoid receptor mRNA in skeletal muscle from patients with Type II diabetes. Clinical Science, 101, 533-540
Wagner J R, Schinckel A P, Chen W, Forrest J C, Coe B L (1999): Analysis of body composition changes of swine during growth and development.. Journal of Animal Science, 77, 1442-
Weinstein Steven P., Wilson Cindy M., Pritsker Alla, Cushman Samuel W. (1998): Dexamethasone inhibits insulin-stimulated recruitment of GLUt4 to the cell surface in rat skeletal muscle. Metabolism, 47, 3-6
Wood J.D, Nute G.R, Richardson R.I, Whittington F.M, Southwood O, Plastow G, Mansbridge R, da Costa N, Chang K.C (2004): Effects of breed, diet and muscle on fat deposition and eating quality in pigs. Meat Science, 67, 651-667
Wood J.D., Enser M., Fisher A.V., Nute G.R., Sheard P.R., Richardson R.I., Hughes S.I., Whittington F.M. (2008): Fat deposition, fatty acid composition and meat quality: A review. Meat Science, 78, 343-358
Yang Yong, Zhao Chunjiang, Xiao Shan, Zhan He, Du Min, Wu Changxin, Ma Changwei (2010): Lipids deposition, composition and oxidative stability of subcutaneous adipose tissue and Longissimus dorsi muscle in Guizhou mini-pig at different developmental stages. Meat Science, 84, 684-690
ZHOU Jie (2004): Developmental patterns of serum leptin levels, leptin gene expression in adipose tissue and Ob-Rb gene expression in hypothalamus of Erhualian and Large White pigs. Science in China Series C, 47, 190-
Zuo Jianjun, Dai Fawen, Feng Dingyuan, Cao Qingyun, Ye Hui, Dong Zemin, Xia Weiguang (2010): Molecular Cloning and mRNA Expression of the Porcine Insulin-responsive Glucose Transporter (GLUT4). Asian-Australasian Journal of Animal Sciences, 23, 640-648
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