Inclusion of Hermetia illucens larvae reared on fish offal to the diet of broiler quails: Effect on immunity and caecal microbial populations
Daniela Pasotto
, Liesel van Emmenes, Marco Cullere, Valerio Giaccone, Elsje Pieterse, Louwrens Christiaan Hoffman, Antonella Dalle Zotte
https://doi.org/10.17221/60/2020-CJASCitation:Pasotto D., van Emmenes L., Cullere M., Giaccone V., Pieterse E., Hoffman L.C., Dalle Zotte A. (2020): Inclusion of Hermetia illucens larvae reared on fish offal to the diet of broiler quails: Effect on immunity and caecal microbial populations. Czech J. Anim. Sci., 65: 213-223.Hermetia illucens (black soldier fly, BSF) larvae meal has shown to be a good protein source in monogastric animal diets, but published data regarding its immunomodulatory properties is limited. For this purpose, a study has been conducted to evaluate the effects of larvae meal on selected immune parameters and caecal bacterial counts of broiler quails. Hermetia illucens larvae were reared on two substrates (100% chicken feed, BSF-M, or 50% chicken feed + 50% fish offal, BSF-F) in order to manipulate the fatty acid profile of larvae meal by increasing the long-chain omega-3 (n-3) polyunsaturated fatty acids which are known for their immunomodulatory properties. For immunological purposes, a total of 60 birds were randomly allocated to three dietary treatment groups (n = 20/treatment): control quail diet (CON), or quail diets including 10% of BSF-F or BSF-M larvae meal. Blood was collected 27 and 37 days after the trial commenced to determine the humoral immune response, serum lysozyme concentrations, serum bactericidal activity and protein fractions of the serum. The dietary inclusion of BSF-M meal resulted in an increased secondary humoral immune response compared to the CON treatment group (P < 0.01). Quails in the BSF-F treatment had significantly higher serum lysozyme activity compared to quails in the CON and BSF-M treatment group. Both larvae meals significantly increased cell-mediated immunity on day 37 (P < 0.001) but they had no effect on serum bactericidal activity. Both larvae meal sources resulted in higher α2-globulin levels on both sampling days, whereas γ-globulin levels were significantly lower in quails with the BSF-F treatment on day 27. Dietary treatments had no significant effect on caecal bacterial counts. In conclusion, the present study showed that the larvae meal had immunostimulatory effects in quails, but the rearing substrate of larvae strongly influenced the immunostimulatory properties of the larvae meal.
insect meal; black soldier fly; humoral immunity; cellular immunity; lysozyme; bactericidal activity; serum protein
References:Impact Factor (Web of Science):
2020: 1.267
Q3 – Agriculture, Dairy and Animal Science
5-Year Impact Factor: 1.428
SCImago Journal Rank (SCOPUS):
New Issue Alert
Join the journal on Facebook!
Ask for email notification.
Abstracted / Indexed in
AGRICOLA
Agrindex of AGRIS/FAO database
Animal Breeding Abstracts
CAB Abstracts
CNKI
Current Contents®/Agriculture, Biology and Environmental Sciences
Czech Agricultural and Food Bibliography
DOAJ (Directory of Open Access Journals)
EBSCO – Academic Search Ultimate
Food Science and Technology Abstracts
Google Scholar
ISI Web of Knowledge®
J-Gate
ProQuest
Science Citation Index Expanded®
SCOPUS
TOXLINE PLUS
Web of Science®
Licence terms
All content is made freely available for non-commercial purposes, users are allowed to copy and redistribute the material, transform, and build upon the material as long as they cite the source.
Open Access Policy
This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.
Contact
Ing. Karolina Tučková
Executive Editor (Editorial Office – publication)
e-mail: cjas
Address
Czech Journal of Animal Science
Czech Academy of Agricultural Sciences
Slezská 7
120 00 Praha 2
Czech Republic