Analysis of cashmere goat meat by label-free proteomics shows that MYL3 is a potential molecular marker of meat toughness

Xie Y.C., Rile N., Li X.W., Li H.J., Zhao M., Che T.Y., Cai T., Liu Z.H., Li J.Q. (2022): Analysis of cashmere goat meat by label-free proteomics shows that MYL3 is a potential molecular marker of meat toughness. Czech J. Anim. Sci., 67: 137–146.

download PDF

The Inner Mongolia cashmere goat is famous for its bright white cashmere fibre. However, little attention is given to the excellent characteristics of this breed’s meat. We used label-free proteomics to analyse the total protein content in five different muscles, and 1 227 proteins were detected. Through sequential windowed acquisition of all theoretical fragment ions (SWATH), 16, 33, 49, 39, and 31 differentially expressed proteins were successfully detected in the five muscles. Protein–protein interaction network analysis of differentially expressed proteins revealed many strong interactions related to fatty acid beta oxidation and muscle development. Based on SWATH in five muscles, 25 differentially expressed proteins related to muscle development were detected, including seven muscle fibre structural proteins (ACTG2, ACTN4, TAGLN, MYL3, MYL1, MYL6B and MYH4). Finally, immunohistochemical analysis of MYL3 showed that the proportion of MYL3 may be a potential molecular marker for muscle toughness.

Burguiere AC, Nord H, von Hofsten J. Alkali-like myosin light chain-1 (myl1) is an early marker for differentiating fast muscle cells in zebrafish. Dev Dyn. 2011 Jul;240(7):1856-63.
Calnan HB, Jacob RH, Pethick DW, Gardner GE. Selection for intramuscular fat and lean meat yield will improve the bloomed colour of Australian lamb loin meat. Meat Sci. 2017 Sep 1;131:187-95.
Cho ES, Lee KT, Kim JM, Lee SW, Jeon HJ, Lee SH, Hong KC, Kim TH. Association of a single nucleotide polymorphism in the 5’ upstream region of the porcine myosin heavy chain 4 gene with meat quality traits in pigs. Anim Sci J. 2016 Mar;87(3):330-5.
Ebhardt HA, Degen S, Tadini V, Schilb A, Johns N, Greig CA, Fearon KC, Aebersold R, Jacobi C. Comprehensive proteome analysis of human skeletal muscle in cachexia and sarcopenia: A pilot study. J Cachexia Sarcopenia Muscle. 2017 Aug;8(4):567-82.
Gao P, Cheng Z, Li M, Zhang N, Le B, Zhang W, Song P, Guo X, Li B, Cao G. Selection of candidate genes affecting meat quality and preliminary exploration of related molecular mechanisms in the Mashen pig. Asian-Australas J Anim Sci. 2019 Aug;32(8):1084-94.
Huang H, Larsen MR, Palmisano G, Dai J, Lametsch R. Quantitative phosphoproteomic analysis of porcine muscle within 24 h postmortem. J Proteomics. 2014 Jun 25;106:125-39.
Hwang YH, Kim GD, Jeong JY, Hur SJ, Joo ST. The relationship between muscle fiber characteristics and meat quality traits of highly marbled Hanwoo (Korean native cattle) steers. Meat Sci. 2010 Oct;86(2):456-61.
Hwang YH, Joo SH, Bakhsh A, Ismail I, Joo ST. Muscle fiber characteristics and fatty acid compositions of the four major muscles in Korean Native Black goat. Korean J Food Sci Anim Resour. 2017 Dec 31;37(6):948-54.
Joo SH, Lee KW, Hwang YH, Joo ST. Histochemical characteristics in relation to meat quality traits of eight major muscles from Hanwoo steers. Korean J Food Sci Anim Resour. 2017 Oct 31;37(5):716-25.
Kim JM, Lim KS, Ko KB, Ryu YC. Estimation of pork quality in live pigs using biopsied muscle fibre number composition. Meat Sci. 2018 Mar;137:130-3.
Koomkrong N, Gongruttananun N, Boonkaewwan C, Noosud J, Theerawatanasirikul S, Kayan A. Fiber characteristics of pork muscle exhibiting different levels of drip loss. Anim Sci J. 2017 Dec;88(12):2044-9.
Lana A, Zolla L. Proteolysis in meat tenderization from the point of view of each single protein: A proteomic perspective. J Proteomics. 2016 Sep 16;147:85-97.
Liu Y, Huttenhain R, Surinova S, Gillet LC, Mouritsen J, Brunner R, Navarro P, Aebersold R. Quantitative measurements of N-linked glycoproteins in human plasma by SWATH-MS. Proteomics. 2013 Apr;13(8):1247-56.
Luo J, Shen YL, Lei GH, Zhu PK, Jiang ZY, Bai L, Li ZM, Tang QG, Li WX, Zhang HS, Zhu L. Correlation between three glycometabolic-related hormones and muscle glycolysis, as well as meat quality, in three pig breeds. J Sci Food Agric. 2017 Jul;97(9):2706-13.
Malheiros JM, Braga CP, Grove RA, Ribeiro FA, Calkins CR, Adamec J, Chardulo LA. Influence of oxidative damage to proteins on meat tenderness using a proteomics approach. Meat Sci. 2019 Feb;148:64-71.
Polati R, Menini M, Robotti E, Millioni R, Marengo E, Novelli E, Balzan S, Cecconi D. Proteomic changes involved in tenderization of bovine longissimus dorsi muscle during prolonged ageing. Food Chem. 2012 Dec 1;135(3):2052-69.
Rosa AF, Moncau CT, Poleti MD, Fonseca LD, Balieiro JC, Silva SL, Eler JP. Proteome changes of beef in Nellore cattle with different genotypes for tenderness. Meat Sci. 2018 Apr;138:1-9.
Schiaffino S, Reggiani C. Fiber types in mammalian skeletal muscles. Physiol Rev. 2011 Oct;91(4):1447-531.
Shen LY, Luo J, Lei HG, Jiang YZ, Bai L, Li MZ, Tang GQ, Li XW, Zhang SH, Zhu L. Effects of muscle fiber type on glycolytic potential and meat quality traits in different Tibetan pig muscles and their association with glycolysis-related gene expression. Genet Mol Res. 2015 Nov 13;14(4):14366-78.
Stuart CA, Stone WL, Howell ME, Brannon MF, Hall HK, Gibson AL, Stone MH. Myosin content of individual human muscle fibers isolated by laser capture microdissection. Am J Physiol Cell Physiol. 2016 Mar 1;310(5):C381-9.
Sun X, Chen KJ, Berg EP, Newman DJ, Schwartz CA, Keller WL, Carlin KM. Prediction of troponin-T degradation using color image texture features in 10d aged beef longissimus steaks. Meat Sci. 2014 Feb;96(2):837-42.
Wang Z, Shang P, Li Q, Wang L, Chamba Y, Zhang B, Zhang H, Wu C. iTRAQ-based proteomic analysis reveals key proteins affecting muscle growth and lipid deposition in pigs. Sci Rep. 2017 Apr 24;7: 11 p.
Wang S, Luo Z, Zhang Y, Yuan D, Ge W, Wang X. The inconsistent regulation of HOXC13 on different keratins and the regulation mechanism on HOXC13 in cashmere goat (Capra hircus). BMC Genom. 2018 Aug 23;19(1): 16 p.
Wu W, Gao XG, Dai Y, Fu Y, Li XM, Dai RT. Post-mortem changes in sarcoplasmic proteome and its relationship to meat color traits in M. semitendinosus of Chinese Luxi yellow cattle. Food Res Int. 2015a Jun;72:98-105.
Wu F, Zuo JJ, Yu QP, Zou SG, Tan HZ, Xiao J, Liu YH, Feng DY. Effect of skeletal muscle fibres on porcine meat quality at different stages of growth. Genet Mol Res. 2015b Jul 14;14(3):7873-82.
Yu TY, Morton JD, Clerens S, Dyer JM. Data for in-depth characterisation of the lamb meat proteome from longissimus lumborum. Data Brief. 2015 Feb 20;3:143-8.
Yu Q, Tian X, Shao L, Xu L, Dai R, Li X. Label-free proteomic strategy to compare the proteome differences between longissimus lumborum and psoas major muscles during early postmortem periods. Food Chem. 2018 Dec 15;269:427-35.
Zhang Q, Lee HG, Han JA, Kim EB, Kang SK, Yin J, Baik M, Shen Y, Kim SH, Seo KS, Choi YJ. Differentially expressed proteins during fat accumulation in bovine skeletal muscle. Meat Sci. 2010 Nov 1;86(3):814-20.
Zhang CZ, Sun HZ, Li SL, Sang D, Zhang CH, Jin L, Antonini M, Zhao CF. Effects of photoperiod on nutrient digestibility, hair follicle activity and cashmere quality in Inner Mongolia white cashmere goats. Asian-Australas J Anim Sci. 2019 Apr;32(4):541-7.
Zhao J, Li J, Su R, Liu Z, Zhang Y, Wang L. Study on muscle nutritional characteristics of Inner Mongolia white cashmere goat. Feed Ind. 2014 Jan;35(1):52-4. Chinese.
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti