Immunological attributes of blood and milk neutrophils isolated from crossbred cows during different physiological conditions
M. Alhussien, P. Manjari, A.A. Sheikh, S. Mohammed Seman, S. Reddi, A.K. Mohanty, J. Mukherjee, A.K. Danghttps://doi.org/10.17221/63/2015-CJASCitation:Alhussien M., Manjari P., Sheikh A.A., Mohammed Seman S., Reddi S., Mohanty A.K., Mukherjee J., Dang A.K. (2016): Immunological attributes of blood and milk neutrophils isolated from crossbred cows during different physiological conditions. Czech J. Anim. Sci., 61: 223-231.
The goal of this work was to understand how polymorphonuclear leukocytes (PMN), the first line of cellular defence, integrate and prioritize multiple chemotactic signals to navigate during periods of demand like subclinical mastitis (SCM), clinical mastitis (CM), pregnancy (P), and calving (C). For this, blood and milk samples were collected from five groups of crossbred cows (healthy, with SCM, CM, pregnant, and newly calved). Maximum viability was seen in the PMN of healthy cows, whereas minimum viability was observed in CM cows. Phagocytic activity (PA) of blood and milk PMN decreased significantly (P < 0.05) at C and in CM cows. Chemotactic activity of blood PMN was minimum in C followed by CM, SCM, and P cows. PA was found to be negatively correlated with the plasma cortisol levels and inverse relationship was observed between the plasma interleukin-8 (IL-8) levels and the chemotactic activity of neutrophils. There was a significantly (P < 0.05) higher expression of CXCR1 and IL-8 on both blood and milk PMN of CM cows followed by C and SCM cows. Minimum expression of selectin (CD-62L) was seen on blood PMN isolated around calving, whereas maximum expression of integrin (CD-11b) was in CM cows. Healthy and P cows showed the highest expression of CD-62L on blood PMN but its expression remained unaltered in milk PMN. Irrespective of physiological stage of the cows, immune suppression was found to be always cortisol dependent. Observing the neutrophil activity and mRNA expression of genes isolated from cow neutrophils can be used as indicators to assess the health/physiological status promptly for immediate therapeutic or management-related actions.Keywords:
phagocytic; chemotactic activity; cortisol; IL-8; CXCR1; selectin; integrinReferences:
REFERENCESAyoub I.A., Bendal R.B., Yang T.J. (1996): Increase in the proportion of CD4+ T-lymphocytes and the levels of transforming growth factor-β in the milk of mastitic cows. Immunology Techniques in Infectious Diseases, 6, 145–150.Blecha F., Baker P.E. (1986): Effect of cortisol in vitro and in vivo on production of bovine interleukin-2. American Journal of Veterinary Research, 47, 841–849.Choi Eun-Mi, Kim Ah-Jin, Kim Yeon-O, Hwang Jae-Kwan (2005): Immunomodulating Activity of Arabinogalactan and Fucoidan In Vitro. Journal of Medicinal Food, 8, 446-453 https://doi.org/10.1089/jmf.2005.8.446Dang A.K., Kapila S., Singh C., Sehgal J.P. (2008): Milk differential cell counts and compositional changes in cows during different physiological stages. Milchwissenschaft, 63, 239–242.DeForge L.E., Preston A.M., Takeuchi E., Kenney J., Boxer L.A., Remick D.G. (1993): Regulation of interleukin 8 gene expression by oxidant stress. Journal of Biological Research, 268, 25568–25576.Diez-Fraille Araceli, Mehrzad Jalil, Meyer Evelyne, Duchateau Luc, Burvenich Christian (2004): Comparison of L-selectin and Mac-1 expression on blood and milk neutrophils during experimental Escherichia coli-induced mastitis in cows. American Journal of Veterinary Research, 65, 1164-1171 https://doi.org/10.2460/ajvr.2004.65.1164DOSOGNE HILDE, VANGROENWEGHE FRÉDÉRIC, BARRIO BELEN, RAINARD PASCAL, BURVENICH CHRISTIAN (2001): Decreased number and bactericidal activity against Staphylococcus aureus of the resident cells in milk of dairy cows during early lactation. Journal of Dairy Research, 68, - https://doi.org/10.1017/S0022029901005088Elner V.M., Streiter R.M., Elner S.G., Baggionlini M., Lindley I., Kunkel S.L. (1990): Neutrophil chemotactic factor (IL-8) gene expression by cytokine-treated retinal pigment epithelial cells. American Journal of Pathology, 136, 745–750.Galvão K.N., Pighetti G.M., Cheong S.H., Nydam D.V., Gilbert R.O. (2011): Association between interleukin-8 receptor-α (CXCR1) polymorphism and disease incidence, production, reproduction, and survival in Holstein cows. Journal of Dairy Science, 94, 2083-2091 https://doi.org/10.3168/jds.2010-3636Glynn P.C., Henney E., Hall I.P. (2002): The selective CXCR2 antagonist SB272844 blocks interleukin-8 and growth-related oncogene-[alpha]-mediated inhibition of spontaneous neutrophil apoptosis. Pulmonary Pharmacology and Therapeutics, 15, 103−111.Jinquan T., Deleyran B., Gesser B., Maare H., Delevran M., Larsen C.G., Thestrup-Pederson K. (1995): Regulation of human T-lymphocyte chemotaxis in vitro by T-cell derived cytokines IL-2, IFN-γ, IL-4, IL-10, and IL-13. Journal of Immunology, 154, 3742–3752.Kasama T., Streiter R.M., Lukacs N.W., Burdick M.D., Kunkel S.L. (1994): Regulation of neutrophil-derived chemokine expression by IL-10. Journal of Immunology, 152, 3559–3569.Kehrli Marcus E., Shuster Dale E. (1994): Factors Affecting Milk Somatic Cells and Their Role in Health of the Bovine Mammary Gland. Journal of Dairy Science, 77, 619-627 https://doi.org/10.3168/jds.S0022-0302(94)76992-7Meglia G.E., Johannisson A., Agenäs S., Holtenius K., Waller K.Persson (2005): Effects of feeding intensity during the dry period on leukocyte and lymphocyte sub-populations, neutrophil function and health in periparturient dairy cows. The Veterinary Journal, 169, 376-384 https://doi.org/10.1016/j.tvjl.2004.02.003Mehrzad Jalil, Duchateau Luc, Pyörälä Satu, Burvenich Christian (2002): Blood and Milk Neutrophil Chemiluminescence and Viability in Primiparous and Pluriparous Dairy Cows During Late Pregnancy, Around Parturition and Early Lactation. Journal of Dairy Science, 85, 3268-3276 https://doi.org/10.3168/jds.S0022-0302(02)74415-9MEHRZAD J, DUCHATEAU L, BURVENICH C (2009): Phagocytic and bactericidal activity of blood and milk-resident neutrophils against Staphylococcus aureus in primiparous and multiparous cows during early lactation. Veterinary Microbiology, 134, 106-112 https://doi.org/10.1016/j.vetmic.2008.09.001Mukherjee R., Jadhav R.K., De U.K. (2010): Expression of L selectin molecule on peripheral leukocyte in response to nisin treatment during acute bovine mastitis. Veterinarski Arhiv, 80, 355–364.Nonnecke B.J., Burton J.L., Kehrli M.E. (1997): Associations Between Function and Composition of Blood Mononuclear Leukocyte Populations from Holstein Bulls Treated with Dexamethasone. Journal of Dairy Science, 80, 2403-2410 https://doi.org/10.3168/jds.S0022-0302(97)76192-7Olson Timothy S., Ley Klaus (): Chemokines and chemokine receptors in leukocyte trafficking. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, 283, R7-R28 https://doi.org/10.1152/ajpregu.00738.2001(2003): The bovine neutrophil: Structure and function in blood and milk. Veterinary Research, 34, 597-627 https://doi.org/10.1051/vetres:2003024Pfaffl M.W. (2001): A new mathematic model for relative quantification in real-time RT-PCR. Nucleic Acids Research, 29, e45.Pfaffl M. W. (): Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research, 30, 36e-36 https://doi.org/10.1093/nar/30.9.e36Qi J., Kreutzer D.L. (1995): Fibrin activation of vascular endothelial cells: induction of IL-8 expression. Journal of Immunology, 155, 867–876.Rainard Pascal, Riollet Céline (2006): Innate immunity of the bovine mammary gland. Veterinary Research, 37, 369-400 https://doi.org/10.1051/vetres:2006007Sugisawa H., Itou T., Saito M., Moritomo T., Miura Y., Sakai T. (2003): A low-molecular-weight fraction of bovine colostrum and milk enhances the oxidative burst activity of polymorphonuclear leukocytes. Veterinary Research Communication, 27, 453–461. https://doi.org/10.1023/A:1025737623792Suriyasathaporn W., Schukken Y.H., Nielen M., Brand A. (2000): Low Somatic Cell Count: a Risk Factor for Subsequent Clinical Mastitis in a Dairy Herd. Journal of Dairy Science, 83, 1248-1255 https://doi.org/10.3168/jds.S0022-0302(00)74991-5Tizard I.R. (2000): Veterinary Immunology. W.B. Saunders Company, Philadelphia, USA.Verbeke Joren, Boulougouris Xanthippe, Rogiers Carolien, Burvenich Christian, Peelman Luc, De Spiegeleer Bart, De Vliegher Sarne (2015): Reactive oxygen species generation by bovine blood neutrophils with different CXCR1 (IL8RA) genotype following Interleukin-8 incubation. BMC Veterinary Research, 11, - https://doi.org/10.1186/s12917-015-0418-5Webster Jeanette I., Tonelli Leonardo, Sternberg Esther M. (2002): N
EUROENDOCRINE R EGULATION OF I MMUNITY *. Annual Review of Immunology, 20, 125-163 https://doi.org/10.1146/annurev.immunol.20.082401.104914Zicha D., Dunn G.A., Jones G.E. (1997): Analyzing chemotaxis using the Dunn direct-viewing chamber. Methods in Molecular Biology, 75, 449–457.