Effect of two freezing extenders on characteristic of fresh and frozen-thawed semen in endangered Old Kladruber stallions – A pilot study

https://doi.org/10.17221/76/2016-CJASCitation:Šichtař J., Nehasilová A., Šimoník O., Bubeníčková F. (2017): Effect of two freezing extenders on characteristic of fresh and frozen-thawed semen in endangered Old Kladruber stallions – A pilot study. Czech J. Anim. Sci., 62: 227-233.
supplementary materialdownload PDF

The aim of the study was to evaluate the effect of two different extenders on sperm characteristic before equilibration and post-thaw in the endangered Old Kladruber stallions. Also, the response of individual stallions to the extenders used was tested. Semen was collected from six stallions every other day within one week. After centrifugation of the collected sperm-rich fraction, the supernatant was removed and sperm pellets were divided to two aliquots; these were diluted either with Gent (Minitube, Germany) or privately manufactured lactose-EDTA-egg yolk extender (Lact). Three cryopreserved insemination doses (IDs) from each extender (Gent and Lact) were prepared for each stallion from one collection (108 samples from six stallions in total). As a parameter of quality, the motility (computer assisted sperm analysis), viability (fluorescence staining), and morphology (eosin/nigrosine staining) were evaluated after dilution with freezing extenders (fresh) and after thawing (frozen-thawed). The different effects of chosen extenders on the quality of fresh semen were only manifested in higher kinematic parameters of sperm when the Lact extender was used. However, in frozen-thawed samples, the Gent extender yielded significantly better results in all of the evaluated parameters. The representation of sperm subpopulation was significantly influenced by extender in fresh as well as frozen-thawed samples; moreover, we found a significant effect of freezing on the distribution of these subpopulations. The response of individual stallions to chosen extenders was evident in the different quality of fresh as well as frozen-thawed IDs; Gent extender yielded better frozen-thawed IDs. Based on our results, among others describing quality parameters of ejaculate in endangered Old Kladruber stallions, we can recommend using Gent extender for the production of frozen-thawed IDs.  

Alvarenga M.A., Papa F.O., Landim-Alvarenga F.C., Medeiros A.S.L (2005): Amides as cryoprotectants for freezing stallion semen: A review. Animal Reproduction Science, 89, 105-113  https://doi.org/10.1016/j.anireprosci.2005.07.001
Brito Leonardo F.C., Greene Lauren M., Kelleman Audrey, Knobbe Marc, Turner Regina (2011): Effect of method and clinician on stallion sperm morphology evaluation. Theriogenology, 76, 745-750  https://doi.org/10.1016/j.theriogenology.2011.04.007
De Geyter C., De Geyter M., Koppers B., Nieschlag E. (1998): Diagnostic accuracy of computer-assisted sperm motion analysis. Human Reproduction, 13, 2512-2520  https://doi.org/10.1093/humrep/13.9.2512
Folková P., Šichtař J., Šimoník O., Dokoupilová A., Rajmon R. (2016): Changes in Quality of Native and Frozenthawed Semen in Relation to Two Collections Performed in a 24-hour Interval and Adition of Clarified Egg Yolk to Extender. Scientia Agriculturae Bohemica, 47, -  https://doi.org/10.1515/sab-2016-0010
Gibb Zamira, Lambourne Sarah R., Aitken Robert J. (2014): The Paradoxical Relationship Between Stallion Fertility and Oxidative Stress1. Biology of Reproduction, 91, -  https://doi.org/10.1095/biolreprod.114.118539
Graham James K. (1996): Cryopreservation of Stallion Spermatozoa. Veterinary Clinics of North America: Equine Practice, 12, 131-147  https://doi.org/10.1016/S0749-0739(17)30300-0
Hofmanová B., Vostrý L., Majzlík I., Vostrá-Vydrová H. (): Characterization of greying, melanoma, and vitiligo quantitative inheritance in Old Kladruber horses. Czech Journal of Animal Science, 60, 443-451  https://doi.org/10.17221/8524-CJAS
Jerez Rodrigo, González Noelia, Olaciregui Maite, Luño Victoria, Blas Ignacio de, Gil Lydia (2016): Use of soy milk combined with different cryoprotectants for the ram semen cryopreservation. Small Ruminant Research, 134, 34-38  https://doi.org/10.1016/j.smallrumres.2015.12.003
Katila T. (2001): In vitro evaluation of frozen-thawed stallion semen: a review. Acta Veterinaria Scandinavica, 42, 199–217. https://doi.org/10.1186/1751-0147-42-199
Loomis P.R., Graham J.K. (2008): Commercial semen freezing: Individual male variation in cryosurvival and the response of stallion sperm to customized freezing protocols. Animal Reproduction Science, 105, 119-128  https://doi.org/10.1016/j.anireprosci.2007.11.010
Mantovani Roberto, Rota Ada, Falomo Maria Elena, Bailoni Lucia, Vincenti Leila (2002): Comparison between glycerol and ethylene glycol for the cryopreservation of equine spermatozoa: semen quality assessment with standard analyses and with the hypoosmotic swelling test. Reproduction Nutrition Development, 42, 217-226  https://doi.org/10.1051/rnd:2002020
Morillo Rodríguez A., Ortega Ferrusola C., Macías García B., Morrell J.M., Rodríguez Martínez H., Tapia J.A., Peña F.J. (2011): Freezing stallion semen with the new Cáceres extender improves post thaw sperm quality and diminishes stallion-to-stallion variability. Animal Reproduction Science, 127, 78-83  https://doi.org/10.1016/j.anireprosci.2011.07.009
Morrell Jane M. (2012): Stallion Sperm Selection: Past, Present, and Future Trends. Journal of Equine Veterinary Science, 32, 436-440  https://doi.org/10.1016/j.jevs.2012.05.069
Muchlisin Z.A., Nadiah W.N., Nadiya N., Fadli N., Hendri A., Khalil M., Siti-Azizah M.N. (): Exploration of natural cryoprotectants for cryopreservation of African catfish, Clarias gariepinus, Burchell 1822 (Pisces: Clariidae) spermatozoa. Czech Journal of Animal Science, 60, 10-15  https://doi.org/10.17221/7906-CJAS
Ortega-Ferrusola C, Macías García B, Suárez Rama V, Gallardo-Bolaños JM, González-Fernández L, Tapia JA, Rodríguez-Martinez H, Peña FJ (2009): Identification of Sperm Subpopulations in Stallion Ejaculates: Changes after Cryopreservation and Comparison with Traditional Statistics. Reproduction in Domestic Animals, 44, 419-423  https://doi.org/10.1111/j.1439-0531.2008.01097.x
Pena F.J., Macias Garcia B., Samper J.C., Aparicio I.M., Tapia J.A., Ortega Ferrusola C. (2011): Dissecting the molecular damage to stallion spermatozoa: The way to improve current cryopreservation protocols? Theriogenology, 76, 1177–1186.
Phetudomsinsuk K., Sirinarumitr K., Choothesa A., Suthanmapinunt P., Kornkaewrat K., Laikul A., Amornsak S., Pinyopummin A. (2009): Freezability of Thai native crossbred horse semen in different extenders. Thai Journal of Veterinary Medicine, 39, 105–114.
Pugliesi Guilherme, Carvalho Giovanni Ribeiro de, Rates Daniel Macêdo, Ker Pedro Gama, Matta Manuela Pereira da, Oliveira Renan Reis de, Silva Filho José Monteiro da (2012): Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders. Revista Brasileira de Zootecnia, 41, 2411-2417  https://doi.org/10.1590/S1516-35982012001200005
Pukazhenthi Budhan S., Johnson Aime, Guthrie H. David, Songsasen Nucharin, Padilla Luis R., Wolfe Barbara A., Coutinho da Silva Marco, Alvarenga Marco A., Wildt David E. (2014): Improved sperm cryosurvival in diluents containing amides versus glycerol in the Przewalski’s horse (Equus ferus przewalskii). Cryobiology, 68, 205-214  https://doi.org/10.1016/j.cryobiol.2014.01.013
Quintero-Moreno A, Miró J, Teresa Rigau A, Rodrı́guez-Gil J.E (2003): Identification of sperm subpopulations with specific motility characteristics in stallion ejaculates. Theriogenology, 59, 1973-1990  https://doi.org/10.1016/S0093-691X(02)01297-9
Rezagholizadeh Alireza, Gharagozlou Faramarz, Niasari-Naslaji Amir, Akbarinejad Vahid, Ziapour Soudeh (2015): Evaluation of Sperm Characteristics in Caspian Stallions Using Computer-Assisted Sperm Analysis. Journal of Equine Veterinary Science, 35, 505-509  https://doi.org/10.1016/j.jevs.2015.02.003
Roldan ERS, Gomendio M, Garde JJ, Espeso G, Ledda S, Berlinguer F, del Olmo A, Soler AJ, Arregui L, Crespo C, González R (2006): Inbreeding and Reproduction in Endangered Ungulates: Preservation of Genetic Variation through the Organization of Genetic Resource Banks. Reproduction in Domestic Animals, 41, 82-92  https://doi.org/10.1111/j.1439-0531.2006.00772.x
Simonik O., Sichtar J., Krejcarkova A., Rajmon R., Stadnik L., Beran J., Dolezalova M., Biniova Z. (2015): Computer assisted sperm analysis – the relationship to bull field fertility, possible errors and their impact on outputs: a review. Indian Journal of Animal Sciences, 85, 3–11.
Squires E. (2013): Semen cryopreservation – challenges and perspectives. Revista Brasileira de Reproducao Animal, 37, 136–139.
Verstegen J., Iguer-Ouada M., Onclin K. (2002): Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology, 57, 149-179  https://doi.org/10.1016/S0093-691X(01)00664-1
Vostrá-Vydrová H., Vostrý L., Hofmanová B., Krupa E., Zavadilová L. (2016): Pedigree analysis of the endangered Old Kladruber horse population. Livestock Science, 185, 17-23  https://doi.org/10.1016/j.livsci.2016.01.001
Zhang X.-G., Hong J.-Y., Yan G.-J., Wang Y.-F., Li Q.-V., Hu J.-H. (): Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls. Czech Journal of Animal Science, 60, 256-262  https://doi.org/10.17221/8239-CJAS
supplementary materialdownload PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti