Effects of folic acid supplementation to basal diets of broilers on growth performance, slaughter performance, IGF2 gene expression and methylation

https://doi.org/10.17221/76/2021-CJASCitation:

Li X.L, Zhang Y.J., Jing W.Q., Tang W.Q., Xing J.Y., Zhang Y.Y. (2021): Effects of folic acid supplementation to basal diets of broilers on growth performance, slaughter performance, IGF2 gene expression and methylation. Czech J. Anim. Sci. 66 (2021): 504-512.

download PDF

Folic acid (FA) is an important water-soluble vitamin and plays an important role as a cofactor and coenzyme in animal growth and development, and regulation of gene expression and methylation. A total of 270 female broiler chickens (1-day-old) were randomly allotted to three dietary treatments supplemented with 0 mg/kg (control group), 5 mg/kg, and 10 mg/kg FA in basal diets for 42 days, respectively. Each treatment had six replicate cages with 15 birds per cage. Dietary supplementation of 5 mg/kg FA significantly enhanced average body weight and average daily gain of 21-day-old broilers (P < 0.05), but significantly reduced subcutaneous fat thickness and widths of an intermuscular fat band of 42-day-old broilers by dietary FA treatments (P < 0.05). Also, a diet with 10 mg/kg FA supplementation significantly increased the relative heart weight of 42-day-old chickens (P < 0.05). Furthermore, dietary FA supplementation significantly improved the serum insulin-like growth factor 2 (IGF2) concentrations (P < 0.01) and IGF2 mRNA expression in the abdominal fat (P < 0.05), but no statistical differences were found in the methylation of IGF2 promoter (P > 0.05). The present study demonstrated that dietary FA supplementation may have positive effects on chicken growth through increased IGF2 gene expression.

 

References:
Bailey LB, Stover PJ, McNulty H, Fenech MF, Gregory JF, Mills JL, Pfeiffer CM, Fazili Z, Zhang M, Ueland PM. Biomarkers of nutrition for development – Folate review. J Nutr. 2015 Jul 1;145(7):1636S-80S. https://doi.org/10.3945/jn.114.206599
 
Crott JW. Effects of altered parental folate and one-carbon nutrient status on offspring growth and metabolism. Mol Aspects Med. 2017 Feb 1;53:28-35. https://doi.org/10.1016/j.mam.2016.11.001
 
Czeizel AE, Dudas I, Vereczkey A, Banhidy F. Folate deficiency and folic acid supplementation: The prevention of neural-tube defects and congenital heart defects. Nutrients. 2013 Nov;5(11):4760-75. https://doi.org/10.3390/nu5114760
 
Fouad AM, El-Senousey HK. Nutritional factors affecting abdominal fat deposition in poultry: A review. Asian-Australas J Anim Sci. 2014 Jul;27(7):1057-68. https://doi.org/10.5713/ajas.2013.13702
 
Friso S, Udali S, De Santis D, Choi SW. One-carbon metabolism and epigenetics. Mol Aspects Med. 2017 Apr;54:28-36. https://doi.org/10.1016/j.mam.2016.11.007
 
Haggarty P, Hoad G, Campbell DM, Horgan GW, Piyathilake C, McNeill G. Folate in pregnancy and imprinted gene and repeat element methylation in the offspring. Am J Clin Nutr. 2013 Jan 1;97(1):94-9. https://doi.org/10.3945/ajcn.112.042572
 
Hoyo C, Fortner K, Murtha AP, Schildkraut JM, Soubry A, Demark-Wahnefried W, Jirtle RL, Kurtzberg J, Forman MR, Overcash F, Huang ZQ. Association of cord blood methylation fractions at imprinted insulin-like growth factor 2 (IGF2), plasma IGF2, and birth weight. Cancer Causes Control. 2012 Apr;23(4):635-45. https://doi.org/10.1007/s10552-012-9932-y
 
Huang RC, Galati JC, Burrows S, Beilin LJ, Li X, Pennell CE, van Eekelen J, Mori TA, Adams LA, Craig JM. DNA methylation of the IGF2/H19 imprinting control region and adiposity distribution in young adults. Clin Epigenetics. 2012 Nov 13;4(1): 11 p. https://doi.org/10.1186/1868-7083-4-21
 
Jing M, Munyaka PM, Tactacan GB, Rodriguez-Lecompte JC, House JD. Performance, serum biochemical responses, and gene expression of intestinal folate transporters of young and older laying hens in response to dietary folic acid supplementation and challenge with Escherichia coli lipopolysaccharide. Poult Sci. 2014 Jan 1;93(1):122-31. https://doi.org/10.3382/ps.2013-03384
 
Kim YI, Pogribny IP, Basnakian AG, Miller JW, Mason JB. Folate deficiency in rats induces DNA strand breaks and hypomethylation within the p53 tumor suppressor gene. Am J Clin Nutr. 1997 Jan 1;65(1):46-52. https://doi.org/10.1093/ajcn/65.1.46
 
Kolbadinejad A, Rezaeipour V. Efficacy of ajwain (Trachyspermum ammi L.) seed at graded levels of dietary threonine on growth performance, serum metabolites, intestinal morphology and microbial population in broiler chickens. J Anim Physiol Nutr. 2020 Sep;104(5):1333-42. https://doi.org/10.1111/jpn.13357
 
Li ZH, Li H, Wang QG, Zhan JG, Wang YX. The study on correlation analysis of single nucleotide polymorphism of IGF2 gene and body fatness traits in chicken. Agr Sci China. 2004 Jan 1;3(10):789-94.
 
Lightfoot TJ, Skibola CF, Willett EV, Skibola DR, Allan JM, Coppede F, Adamson PJ, Morgan GJ, Roman E, Smith MT. Risk of non-Hodgkin lymphoma associated with polymorphisms in folate-metabolizing genes. Cancer Epidemiol Biomarkers Prev. 2005 Dec 1;14(12):2999-3003. https://doi.org/10.1158/1055-9965.EPI-05-0515
 
Liu JB, Yao J, Yu B, Mao XB, Huang ZQ, Chen DW. Effect of maternal folic acid supplementation on hepatic proteome in newborn piglets. Nutrition. 2013 Jan 1;29(1):230-4. https://doi.org/10.1016/j.nut.2012.08.001
 
Liu YL, Zhi LH, Shen J, Li SZ, Yao JH, Yang XJ. Effect ofin ovo folic acid injection on hepatic IGF2 expression and embryo growth of broilers. J Anim Sci Biotechnol. 2016 Jul 22;7(1): 9 p. https://doi.org/10.1186/s40104-016-0099-3
 
Liu YR, Du HS, Wu ZZ, Wang C, Liu Q, Guo G, Huo WJ, Zhang YL, Pei CX, Zhang SL. Branched-chain volatile fatty acids and folic acid accelerated the growth of Holstein dairy calves by stimulating nutrient digestion and rumen metabolism. Animal. 2020 Jun;14(6):1176-83. https://doi.org/10.1017/S1751731119002969
 
Nazki FH, Sameer AS, Ganaie BA. Folate: Metabolism, genes, polymorphisms and the associated diseases. Gene. 2014 Jan 1;533(1):11-20. https://doi.org/10.1016/j.gene.2013.09.063
 
Pereira SS, Monteiro MP, Costa MM, Moreira A, Alves MG, Oliveira PF, Jarak I, Pignatelli D. IGF2 role in adrenocortical carcinoma biology. Endocrine. 2019 Aug 4;66(2):326-37. https://doi.org/10.1007/s12020-019-02033-5
 
Perkins E, Murphy SK, Murtha AP, Schildkraut J, Jirtle RL, Demark-Wahnefried W, Forman MR, Kurtzberg J, Overcash F, Huang ZQ, Hoyo C. Insulin-like growth factor 2/H19 methylation at birth and risk of overweight and obesity in children. J Pediatrics. 2012 Jul;161(1):31-9. https://doi.org/10.1016/j.jpeds.2012.01.015
 
Sandhu MS, Gibson JM, Heald AH, Dunger DB, Wareham NJ. Low circulating IGF-II concentrations predict weight gain and obesity in humans. Diabetes. 2003 Jun 1;52(6):1403-8. https://doi.org/10.2337/diabetes.52.6.1403
 
Steegers-Theunissen RP, Obermann-Borst SA, Kremer D, Lindemans J, Siebel C, Steegers EA, Slagboom PE, Heijmans BT. Periconceptional maternal folic acid use of 400 μg per day is related to increased methylation of the IGF2 gene in the very young child. PLoS One. 2009 Nov 16;4(11): 5 p. https://doi.org/10.1371/journal.pone.0007845
 
Tactacan GB, Rodriguez-Lecompte JC, House JD. The adaptive transport of folic acid in the intestine of laying hens with increased supplementation of dietary folic acid. Poult Sci. 2012 Jan 1;91(1):121-8. https://doi.org/10.3382/ps.2011-01711
 
Tserga A, Binder AM, Michels KB. Impact of folic acid intake during pregnancy on genomic imprinting of IGF2/H19 and 1-carbon metabolism. FASEB J. 2017 Dec;31(12):5149-58. https://doi.org/10.1096/fj.201601214RR
 
Waterland RA. Post-weaning diet affects genomic imprinting at the insulin-like growth factor 2 (Igf2) locus. Hum Mol Genet. 2006 Mar 1;15(5):705-16. https://doi.org/10.1093/hmg/ddi484
 
Wu S, Guo W, Li X, Liu Y, Li Y, Lei X, Yao J, Yang X. Paternal chronic folate supplementation induced the transgenerational inheritance of acquired developmental and metabolic changes in chickens. Proc R Soc B. 2019 Sep 11;286(1910): 9 p. https://doi.org/10.1098/rspb.2019.1653
 
Xing JY, Kang L, Hu Y, Xu QY, Zhang NB, Jiang YL. Effect of dietary betaine supplementation on mRNA expression and promoter CpG methylation of lipoprotein lipase gene in laying hens. J Poult Sci. 2009 Jul 25;46(3):224-8. https://doi.org/10.2141/jpsa.46.224
 
Xing JY, Jing WQ, Zhang YJ, Liu L, Xu JJ, Chen XW. Identification of differentially expressed genes in broiler offspring under maternal folate deficiency. Physiol Genomics. 2018 Dec 1;50(12):1015-25. https://doi.org/10.1152/physiolgenomics.00086.2018
 
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti