Effect of housing system and age of laying hens on eggshell quality, microbial contamination, and penetration of microorganisms into eggs

 

https://doi.org/10.17221/77/2017-CJASCitation:Vlčková J., Tůmová E., Ketta M., Englmaierová M., Chodová D. (2018): Effect of housing system and age of laying hens on eggshell quality, microbial contamination, and penetration of microorganisms into eggs  . Czech J. Anim. Sci., 63: 51-60.
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Hens of the laying hybrid ISA Brown were used in the study with the objective to evaluate eggshell quality, microbial contamination of eggshells, and penetration of microorganisms into the egg content in different housing systems (enriched cage: 60 hens, 10 hens per cage, 750 cm2 per hen vs free range: 60 hens, 9 hens per m2) and at different hen ages (26 vs 51 weeks) during storage time (0, 2, 7, 14, and 21 days). A significant interaction between the housing system and age was observed in egg weight and most of eggshell quality measurements. However, microbial contamination and penetration were affected mostly by the housing system and storage time. The numbers of Escherichia coli (P < 0.001, 4.51 vs 2.75 log cfu/eggshell) and Enterococcus (P < 0.001, 2.56 vs 1.11 log cfu/eggshell), and the total number of microorganisms (P < 0.001, 5.04 vs. 3.65 log cfu/eggshell) were higher in free range eggs compared to enriched cage eggs, respectively. The counts of Escherichia coli (P < 0.001, 4.23 vs 2.91 log cfu/eggshell) and Enterococcus (P < 0.001, 2.31 vs 1.27 log cfu/eggshell) decreased with storage time. A positive correlation between the total number of pores and penetration of Escherichia coli in both housing systems was observed in the albumen. It can be concluded that the housing system and age of laying hens significantly affected eggshell quality. Microbial contamination presumably affects the penetration of microorganisms. The correlation between the number of pores and penetration is assumed to be affected by the microbial species.

References:
Ahmed A.M.H., Rodriguez-Navarro A.B., Vidal M.L., Gautron J., García-Ruiz J.M., Nys Y. (2010): Changes in eggshell mechanical properties, crystallographic texture and in matrix proteins induced by moult in hens. British Poultry Science, 46, 268-279  https://doi.org/10.1080/00071660500065425
 
Belkot Z., Gondek M. (2014): The bacterial pollution of consumer egg surfaces with regards to the maintenance system of laying hens. Medycyna Weterynaryjna, 70, 378–382.
 
Board R. G., Halls N. A. (2007): The cuticle: A barrier to liquid and particle penetration of the shell of the hen's egg. British Poultry Science, 14, 69-97  https://doi.org/10.1080/00071667308415999
 
De Reu K., Grijspeerdt K., Heyndrickx M., Zoons J., De Baere D., Uyttendaele M., Debevere J., Herman L. (2005a): Bacterial eggshell contamination in conventional cages, furnished cages and aviary housing systems. British Poultry Science, 46, 149–155.
 
De Reu K., Grijspeerdt K., Heyndrickx M., Uyttendaele M., Herman L. (2005b): The use of total aerobic and Gram-negative flora for quality assurance in the production chain of consumption eggs. Food Control, 16, 147–155.
 
De Reu K., Grijspeerdt K., Heyndrickx M., Uyttendaele M., Debevere J., Herman L. (2006a): Bacterial eggshell contamination in the egg collection chains of different housing systems for laying hens. British Poultry Science, 47, 163–172.
 
De Reu K., Grijspeerdt K., Messens W., Heyndrickx M., Uyttendaele M., Debevere J., Herman L. (2006b): Eggshell factors influencing eggshell penetration and whole egg contamination by different bacteria, including Salmonella Enteritidis. International Journal of Food Microbiology, 112, 253–260.
 
De Reu K., Heyndrickx M., Grijspeerdt K., Rodenburg T.B., Tuyttens F., Uyttendaele M., Debevere J., Herman L. (2007): Estimation of the vertical and horizontal bacterial infection of hen’s table eggs. In: Proc. XVII European Symposium on the Quality of Poultry Meat and XII European Symposium on the Quality of Eggs and Egg Products, Prague, Czech Republic, 46–47.
 
Hidalgo A., Rossi M., Clerici F., Ratti S. (2008): A market study on the quality characteristics of eggs from different housing systems. Food Chemistry, 106, 1031-1038  https://doi.org/10.1016/j.foodchem.2007.07.019
 
Huneau-Salaün A., Michel V., Huonnic D., Balaine L., Le Bouquin S. (2010): Factors influencing bacterial eggshell contamination in conventional cages, furnished cages and free-range systems for laying hens under commercial conditions. British Poultry Science, 51, 163-169  https://doi.org/10.1080/00071668.2010.482462
 
Hunton P (2005): Research on eggshell structure and quality: an historical overview. Revista Brasileira de Ciência Avícola, 7, 67-71  https://doi.org/10.1590/S1516-635X2005000200001
 
Jones D., Anderson K., Curtis P., Jones F. (2002): Microbial contamination in inoculated shell eggs: I. Effects of layer strain and hen age. Poultry Science, 81, 715-720  https://doi.org/10.1093/ps/81.5.715
 
Ketta M., Tůmová E. (2016): Eggshell structure, measurements, and quality-affecting factors in laying hens: a review. Czech Journal of Animal Science, 61, 299-309  https://doi.org/10.17221/46/2015-CJAS
 
Kretzschmar-McCluskey V., Curtis P. A., Anderson K. E., Berry W. D., Kerth L. K. (2009): Influence of hen age and strain on eggshell exterior, eggshell interior with membranes, and egg contents of microflora, and on Salmonella incidence during a single production cycle. The Journal of Applied Poultry Research, 18, 665-670  https://doi.org/10.3382/japr.2008-00104
 
Lewko Lidia, Gornowicz Ewa (2011): Effect of Housing System on Egg Quality in Laying Hens. Annals of Animal Science, 11, -  https://doi.org/10.2478/v10220-011-0012-0
 
Lichovnikova M., Zeman L. (2008): Effect of housing system on the calcium requirements of laying hens and eggshell quality. Czech Journal of Animal Science, 53, 162–168.
 
Mallet S., Ahmed A., Guesdon V., Nys Y. (2003): Comparison of eggshell quality and hygiene in two housing systems: standard and furnished cages. In: Proc. 10th European Symposium on the Quality of Eggs and Egg Products, Ploufragan, France, 283–242.
 
Mertens K., Bamelis F., Kemps B., Kamers B., Verhoelst E., De Ketelaere B., Bain M., Decuypere E., De Baerdemaeker J. (2006): Monitoring of Eggshell Breakage and Eggshell Strength in Different Production Chains of Consumption Eggs. Poultry Science, 85, 1670-1677  https://doi.org/10.1093/ps/85.9.1670
 
Messens W., Grijspeerdt K., Herman L. (2005): Eggshell penetration by Salmonella: a review. World's Poultry Science Journal, 61, 71-86  https://doi.org/10.1079/WPS200443
 
MESSENS WINY, GRIJSPEERDT KOEN, DE REU KOEN, DE KETELAERE BART, MERTENS KRISTOF, BAMELIS FLIP, KEMPS BART, DE BAERDEMAEKER JOSSE, DECUYPERE EDDY, HERMAN LIEVE (2007): Eggshell Penetration of Various Types of Hens' Eggs by Salmonella enterica Serovar Enteritidis. Journal of Food Protection, 70, 623-628  https://doi.org/10.4315/0362-028X-70.3.623
 
Nascimento V. P., Cranstoun S., Solomon S. E. (2007): Relationship between shell structure and movement of Salmonella enteritidis across the eggshell wall. British Poultry Science, 33, 37-48  https://doi.org/10.1080/00071669208417442
 
Nys Y., Hincke M.T., Arias J.L., Garcia-Ruiz J.M., Solomon S.E. (1999): Avian eggshell mineralization. Poultry and Avian Biology Reviews, 10, 143–166.
 
Rodriguez-Navarro A., Kalin O., Nys Y., Garcia-Ruiz J.M. (2010): Influence of the microstructure on the shell strength of eggs laid by hens of different ages. British Poultry Science, 43, 395-403  https://doi.org/10.1080/00071660120103675
 
Sauter E. A., Petersen C. F. (1974): The Effect of Egg Shell Quality on Penetration by Various Salmonellae. Poultry Science, 53, 2159-2162  https://doi.org/10.3382/ps.0532159
 
Schwarz G., Kobe A., Fries R. (1999): Microflora on egg shells from different housing systems. Archiv für Geflügelkunde, 63, 220–224.
 
Singh R., Cheng K. M., Silversides F. G. (2009): Production performance and egg quality of four strains of laying hens kept in conventional cages and floor pens. Poultry Science, 88, 256-264  https://doi.org/10.3382/ps.2008-00237
 
Skrivan M., Englmaierova M., Skrivanova E., Bubancova I. (2015): Increase in lutein and zeaxanthin content in the eggs of hens fed marigold flower extract. Czech Journal of Animal Science, 60, 89–96.
 
Svobodová J., Tůmová E., Popelářová E., Chodová D. (2016): Effect of light colour on egg production and egg contamination. Czech Journal of Animal Science, 60, 550-556  https://doi.org/10.17221/8597-CJAS
 
Tumova E., Englmaierova M., Ledvinka Z., Charvatova V. (2011): Interaction between housing system and genotype in relation to internal and external egg quality parameters. Czech Journal of Animal Science, 56, 490–498.
 
Tumova E., Gous R.M., Tyler N. (2014): Effect of hen age, environmental temperature, and oviposition time on egg shell quality and eggshell and serum mineral contents in laying and broiler breeder hens. Czech Journal of Animal Science, 59, 435–443.
 
Brand H. Van Den, Parmentier H. K., Kemp B. (2004): Effects of housing system (outdoor vs cages) and age of laying hens on egg characteristics. British Poultry Science, 45, 745-752  https://doi.org/10.1080/00071660400014283
 
Vucemilo M., Vinkovic B., Matkovic K., Stokovic I., Jaksic S., Radovic S., Granic K., Stubican D. (2010): The influence of housing systems on the air quality and bacterial eggshell contamination of table eggs. Czech Journal of Animal Science, 55, 243–249.
 
Wathes C.M. (1994): Air and surface hygiene. In: Wathes C.M. and Charles D.R. (eds): Livestock Housing. CAB International, Wallingford, UK, 123–148.
 
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