Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility


https://doi.org/10.17221/79/2017-CJASCitation:Slanina T., Miškeje M., Tirpák F., Błaszczyk M., Stawarz R., Massányi P. (2018): Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility  . Czech J. Anim. Sci., 63: 127-135.
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The effect of taurine on the turkey spermatozoa motility and viability during the in vitro incubation was assessed. Experimental samples were prepared by diluting the raw semen in nine different concentrations of taurine – from 10 mg/ml to 0.078125 mg/ml. The motility parameters were evaluated by the CASA system (Computer Assisted Semen Analyser) using the program Sperm Vision® and for spermatozoa viability assessment the eosin-nigrosin staining was performed. Selected parameters were evaluated at six time periods: 0, 1, 2, 3, 4, and 5 h at 5°C and 41°C. At 5°C, a significantly lower percentage of motility and progressive motility was detected only in the samples with the highest concentration of taurine (10 mg/ml) at time 0 and 1. After 2 h of incubation a significant preventive effect of taurine on spermatozoa parameters was observed. The tendency of the taurine effect on motility parameters was different during the in vitro incubation at 41°C. Significantly lower values of motility parameters were detected in all experimental samples in comparison to the control after 5 h. The analysed concentrations of taurine did not significantly affect viability of turkey spermatozoa during all time periods. A higher percentage of dead spermatozoa were observed at 41°C (4.87–9.90%) if compared to 5°C (2.12–4.88%). The results indicated that the addition of taurine (from 2.5 to 7.5 mg/ml) to turkey spermatozoa positively affected the monitored spermatozoa parameters incubated at 5°C.

Alvarez Juan G., Storey Bayard T. (1983): Taurine, Hypotaurine, Epinephrine and Albumin Inhibit Lipid Peroxidation in Rabbit Spermatozoa and Protect Against Loss of Motility. Biology of Reproduction, 29, 548-555  https://doi.org/10.1095/biolreprod29.3.548
Blaszczyk M., Slanina T., Massanyi P., Stawarz R. (2013): Semen quality assessment of New Zealand white rabbit bucks. Journal of Microbiology, Biotechnology and Food Sciences, 2, 2168–2179.
Bucak Mustafa N., Tekin Necmettin (2007): Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen. Small Ruminant Research, 73, 103-108  https://doi.org/10.1016/j.smallrumres.2006.12.001
Bucak Mustafa Numan, Ateşşahin Ahmet, Varışlı Ömer, Yüce Abdurrauf, Tekin Necmettin, Akçay Aytaç (2007): The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen. Theriogenology, 67, 1060-1067  https://doi.org/10.1016/j.theriogenology.2006.12.004
Chhillar Shivani, Singh Vivek Kumar, Kumar Raj, Atreja Suresh Kumar (2012): Effects of Taurine or Trehalose supplementation on functional competence of cryopreserved Karan Fries semen. Animal Reproduction Science, 135, 1-7  https://doi.org/10.1016/j.anireprosci.2012.08.029
Dawson Ralph, Wallace David R. (1992): Taurine content in tissues from aged Fischer 344 rats. AGE, 15, 73-81  https://doi.org/10.1007/BF02435005
FROMAN D. P., McLEAN D. J. (1996): Objective Measurement of Sperm Motility Based Upon Sperm Penetration of Accudenz(R). Poultry Science, 75, 776-784  https://doi.org/10.3382/ps.0750776
Guérin P., Guillaud J., Ménézo Y. (1995): Andrology: Hypotaurine in spermatozoa and genital secretions and its production by oviduct epithelial cells in vitro. Human Reproduction, 10, 866-872  https://doi.org/10.1093/oxfordjournals.humrep.a136052
Gutiérrez-Pérez Oscar, Juárez-Mosqueda María de Lourdes, Carvajal Salvador Uribe, Ortega María Elena Trujillo (2009): Boar spermatozoa cryopreservation in low glycerol/trehalose enriched freezing media improves cellular integrity. Cryobiology, 58, 287-292  https://doi.org/10.1016/j.cryobiol.2009.02.003
Hu J-H, Li Q-W, Jiang Z-L, Yang H, Zhang S-S, Zhao H-W (2009): The Cryoprotective Effect of Trehalose Supplementation on Boar Spermatozoa Quality. Reproduction in Domestic Animals, 44, 571-575  https://doi.org/10.1111/j.1439-0531.2007.00975.x
King L.M., Holsberger D.R., Donoghue A.M. (2000): Correlation of CASA velocity and linearity parameters with sperm mobility phenotype in turkeys. Journal of Andrology, 21, 65–71.
Krockova Jirina, Massányi Peter, Toman Robert, Danko Jan, Roychoudhury Shubhadeep (2012): In vivo and in vitro effect of bendiocarb on rabbit testicular structure and spermatozoa motility. Journal of Environmental Science and Health, Part A, 47, 1301-1311  https://doi.org/10.1080/10934529.2012.672136
Kumar R, Atreja SK (2012): Effect of Incorporation of Additives in Tris-Based Egg Yolk Extender on Buffalo (Bubalus bubalis) Sperm Tyrosine Phosphorylation During Cryopreservation. Reproduction in Domestic Animals, 47, 485-490  https://doi.org/10.1111/j.1439-0531.2011.01908.x
Li Jian Hua, Ling Ya Qin, Fan Jing Jing, Zhang Xiao Ping, Cui Sheng (2006): Expression of cysteine sulfinate decarboxylase (CSD) in male reproductive organs of mice. Histochemistry and Cell Biology, 125, 607-613  https://doi.org/10.1007/s00418-005-0095-8
Lobo Maria V.T., Alonso F. Javier M., del Río Rafael Martín (2016): Immunohistochemical Localization of Taurine in the Male Reproductive Organs of the Rat. Journal of Histochemistry & Cytochemistry, 48, 313-320  https://doi.org/10.1177/002215540004800301
Martínez-Páramo S., Diogo P., Dinis M.T., Soares F., Sarasquete C., Cabrita E. (2013): Effect of two sulfur-containing amino acids, taurine and hypotaurine in European sea bass (Dicentrarchus labrax) sperm cryopreservation. Cryobiology, 66, 333-338  https://doi.org/10.1016/j.cryobiol.2013.04.001
Martins-Bessa A., Rocha A., Mayenco-Aguirre A. (2009): Effects of taurine and hypotaurine supplementation and ionophore concentrations on post-thaw acrosome reaction of dog spermatozoa. Theriogenology, 71, 248-253  https://doi.org/10.1016/j.theriogenology.2008.07.006
Massanyi Peter, Weis Jan, Lukac Norbert, Trandzik Jozef, Bystricka Judita (2008): Cadmium, zinc, copper, sodium and potassium concentrations in rooster and turkey semen and their correlation. Journal of Environmental Science and Health, Part A, 43, 563-565  https://doi.org/10.1080/10934520701796655
Partyka Agnieszka, Rodak Olga, Bajzert Joanna, Kochan Joanna, Niżański Wojciech (2017): The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen. BioMed Research International, 2017, 1-8  https://doi.org/10.1155/2017/7279341
Perumal P., Vupru Kezhavituo, Rajkhowa C. (2013): Effect of Addition of Taurine on the Liquid Storage (5 ° C) of Mithun ( Bos frontalis ) Semen. Veterinary Medicine International, 2013, 1-7  https://doi.org/10.1155/2013/165348
Shiva Shankar Reddy N., Jagan Mohanarao G., Atreja S.K. (2010): Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (Bubalus bubalis) sperm quality following cryopreservation. Animal Reproduction Science, 119, 183-190  https://doi.org/10.1016/j.anireprosci.2010.01.012
Sánchez-Partida L. G., Setchell B. P., Maxwell W. M. C. (1997): Epididymal compounds and antioxidants in diluents for the frozen storage of ram spermatozoa. Reproduction, Fertility and Development, 9, 689-  https://doi.org/10.1071/R97045
Sarıözkan Serpil, Bucak Mustafa Numan, Tuncer Pürhan Barbaros, Ulutaş Pınar Alkım, Bilgen Ali (2009): The influence of cysteine and taurine on microscopic–oxidative stress parameters and fertilizing ability of bull semen following cryopreservation. Cryobiology, 58, 134-138  https://doi.org/10.1016/j.cryobiol.2008.11.006
Slanina T., Miskeje M., Knizat L., Mirda J., Massanyi P. (2012): The effect of different concentration of trehalose on turkey spermatozoa motility in vitro. Journal of Microbiology, Biotechnology and Food Sciences, 4, 573–582.
Slanina T., Miskeje M., Petrovicova I., Lukac N., Massanyi P. (2015a): Changes in turkey spermatozoa motility parameters after addition of copper sulphate in vitro. Journal of Microbiology, Biotechnology and Food Sciences, 4, 98–100.
Slanina T., Petrovicova L., Miskeje M., Knizat L., Mirda J., Lukac N., Trandzik J., Petrovicova I., Massanyi P. (2015b): The effect of diluent, temperature and age on turkey spermatozoa motility in vitro. Journal of Applied Animal Research, 43, 131–136.
Slanina Tomáš, Kováčová Renáta, Lukáč Norbert, Massányi Peter (2016): Impact of tilmicosin on the rabbit spermatozoa motility and viability. Journal of Microbiology, Biotechnology and Food Sciences, 05, 53-56  https://doi.org/10.15414/jmbfs.2016.5.special1.53-56
Witte Tanja Semira, Schäfer-Somi Sabine (2007): Involvement of cholesterol, calcium and progesterone in the induction of capacitation and acrosome reaction of mammalian spermatozoa. Animal Reproduction Science, 102, 181-193  https://doi.org/10.1016/j.anireprosci.2007.07.007
Yang J., Wu G., Feng Y., Lv Q., Lin S., Hu J. (2010a): Effects of taurine on male reproduction in rats of different ages. Journal of Biomedical Science, 17 (Suppl. 1): S9.
Yang J.C., Wu G.F., Feng Y., Sun C.M., Lin S.M., Hu J.M. (2010b): CSD mRNA expression in rat testis and the effect of taurine on testosterone secretion. Amino Acids, 39, 155–160.
Yun Jung Im, Gong Seung Pyo, Song Young Han, Lee Seung Tae (2013): Effects of combined antioxidant supplementation on human sperm motility and morphology during sperm manipulation in vitro. Fertility and Sterility, 100, 373-378  https://doi.org/10.1016/j.fertnstert.2013.04.015
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