Effect of silver nanoparticles on the immune, redox, and lipid status of chicken blood

https://doi.org/10.17221/80/2015-CJASCitation:Ognik K., Cholewińska E., Czech A., Kozłowski K., Wlazło Ł., Nowakowicz-Dębek B., Szlązak R., Tutaj K. (2016): Effect of silver nanoparticles on the immune, redox, and lipid status of chicken blood. Czech J. Anim. Sci., 61: 450-461.
download PDF
The aim of the study was to analyze how per os application of hydrocolloids of silver nanoparticles (22 nm) and lipid-coated silver nanoparticles (5 nm) affect the immune, redox, and lipid status of the blood of broiler chickens. The experiment was conducted on 60 chickens. The first group was the control (Group C). The chickens in Group II received a silver nanoparticle hydrocolloid (Ag-nano) at a dose of 5 mg/kg body weight (BW) per day. The chickens in Group III received a lipid-coated silver nanoparticle hydrocolloid (AgL-nano) at a dose of 5 mg/kg BW per day. Blood for analysis was collected from 24- and 38-day-old chickens and several blood parameters were determined. The increase in phagocytosis and in the metabolic activity of leukocytes observed following per os administration of chemically synthesized silver nanoparticles differing in size and in the presence or absence of a lipid coating may indicate a developing inflammatory state in the organism. The decrease in haemoglobin content and antioxidant enzyme activity and the increase in the content of iron, bilirubin, and lipid peroxidation products are indicative of oxidative stress, although in the case of administration of AgL-nano the oxidation effect appears to be greater. Administration of silver nanoparticles to the chickens, particularly 5 nm lipid-coated nanoparticles, probably led to a disturbance in protein catabolism in the organism, which is evidenced by the decrease in the activity of the liver enzymes AST and ALT and the decreased concentration of the main protein metabolism products (creatinine and urea).
References:
Ahmadi F. (2012): Impact of different levels of silver nanoparticles (Ag-NPs) on performance, oxidative enzymes and blood parameters in broiler chicks. Pakistan Veterinary Journal, 32, 325–328.
 
Ahmadi F., Kurdestani A.H. (2010): The impact of silver nanoparticles on growth performance, lymphoid organs and oxidative stress indicators in broiler chicks. Global Veterinaria, 5, 366–370.
 
(2013): The effect of dietary silver nanoparticles on performance, immune organs, and lipid serum of broiler chickens during starter period. International Journal of Biosciences (IJB), 3, 95-100  https://doi.org/10.12692/ijb/3.5.95-100
 
Andi M.A., Mohsen H., Farhad A. (2011): Effects of feed type with/without nanosil on cumulative performance, relative organ weight and some blood parameters of broilers. Global Veterinaria, 7, 605–609.
 
ARAGON G., YOUNOSSI Z. M. (): When and how to evaluate mildly elevated liver enzymes in apparently healthy patients. Cleveland Clinic Journal of Medicine, 77, 195-204  https://doi.org/10.3949/ccjm.77a.09064
 
Bartosz G. (eds) (2004): Second Face of Oxygen. PWN, Warsaw, Poland.
 
Batzri Shmuel, Korn Edward D. (1973): Single bilayer liposomes prepared without sonication. Biochimica et Biophysica Acta (BBA) - Biomembranes, 298, 1015-1019  https://doi.org/10.1016/0005-2736(73)90408-2
 
Bhanja Subrat, Hotowy Anna, Mehra Manish, Sawosz Ewa, Pineda Lane, Vadalasetty Krishna, Kurantowicz Natalia, Chwalibog André (2015): In Ovo Administration of Silver Nanoparticles and/or Amino Acids Influence Metabolism and Immune Gene Expression in Chicken Embryos. International Journal of Molecular Sciences, 16, 9484-9503  https://doi.org/10.3390/ijms16059484
 
Bylund J., Brown K.L., Movitz C., Dahlgren C., Karlsson A. (2010): Intracellular generation of superoxide by the phagocyte NADPH oxidase: how, where, and what for? Free Radical Biology and Medicine, 49, 1834–1845.
 
Carr A. C., McCall M. R., Frei B. (): Oxidation of LDL by Myeloperoxidase and Reactive Nitrogen Species : Reaction Pathways and Antioxidant Protection. Arteriosclerosis, Thrombosis, and Vascular Biology, 20, 1716-1723  https://doi.org/10.1161/01.ATV.20.7.1716
 
Dobrzanski Z., Zygadlik K., Patkowska-Sokola B., Nowakowski P., Janczak M., Sobczak A., Bodkowski R. (2010): The effectiveness of nanosilver and mineral sorbents in the reduction of ammonia emissions from livestock manure. Przemysł Chemiczny, 4, 348–351. (in Polish)
 
Feldman B., Zinki J., Jain N. (2000): Schalm’s Veterinary Haematology. Lippincott Williams & Wilkins, Philadelphia, USA.
 
Gay Craig A., Gebicki Janusz M. (2002): Perchloric Acid Enhances Sensitivity and Reproducibility of the Ferric–Xylenol Orange Peroxide Assay. Analytical Biochemistry, 304, 42-46  https://doi.org/10.1006/abio.2001.5566
 
Gholami-Ahangaran M., Zia-Jahromi N. (): Effect of nanosilver on blood parameters in chickens having aflatoxicosis. Toxicology and Industrial Health, 30, 192-196  https://doi.org/10.1177/0748233712452611
 
Hersleth Hans-Petter, Hsiao Ya-Wen, Ryde Ulf, Görbitz Carl Henrik, Andersson K. Kristoffer (2008): The crystal structure of peroxymyoglobin generated through cryoradiolytic reduction of myoglobin compound III during data collection. Biochemical Journal, 412, 257-  https://doi.org/10.1042/BJ20070921
 
Jovanović Boris, Palić Dušan (2012): Immunotoxicology of non-functionalized engineered nanoparticles in aquatic organisms with special emphasis on fish—Review of current knowledge, gap identification, and call for further research. Aquatic Toxicology, 118-119, 141-151  https://doi.org/10.1016/j.aquatox.2012.04.005
 
Daniel S.C.G. Kiruba, Tharmaraj V., Sironmani T. Anitha, Pitchumani K. (2010): Toxicity and immunological activity of silver nanoparticles. Applied Clay Science, 48, 547-551  https://doi.org/10.1016/j.clay.2010.03.001
 
Liu Huanliang, Yang Danfeng, Yang Honglian, Zhang Huashan, Zhang Wei, Fang YanJun, Lin Zhiqing, Tian Lei, Lin Bencheng, Yan Jun, Xi Zhuge (2013): Comparative study of respiratory tract immune toxicity induced by three sterilisation nanoparticles: Silver, zinc oxide and titanium dioxide. Journal of Hazardous Materials, 248-249, 478-486  https://doi.org/10.1016/j.jhazmat.2013.01.046
 
Ognik K., Wertelecki T. (2012): Effect of different vitamin E sources and levels on selected oxidative status indices in blood and tissues as well as on rearing performance of slaughter turkey hens. The Journal of Applied Poultry Research, 21, 259-271  https://doi.org/10.3382/japr.2011-00366
 
Ognik Katarzyna, Sembratowicz Iwona, Cholewińska Ewelina, Wlazło Łukasz, Nowakowicz-Dębek Bożena, Szlązak Radosław, Tutaj Krzysztof (2016): 10. The Effect of Chemically-Synthesized Silver Nanoparticles on Performance and the Histology and Microbiological Profile of the Jejunum in Chickens. Annals of Animal Science, 16, -  https://doi.org/10.1515/aoas-2015-0067
 
Oliveira Marcela M., Ugarte Daniel, Zanchet Daniela, Zarbin Aldo J.G. (2005): Influence of synthetic parameters on the size, structure, and stability of dodecanethiol-stabilized silver nanoparticles. Journal of Colloid and Interface Science, 292, 429-435  https://doi.org/10.1016/j.jcis.2005.05.068
 
Park B.H., Fikrig S.M., Smithwick E.M. (1968): INFECTION AND NITROBLUE-TETRAZOLIUM REDUCTION BY NEUTROPHILS. The Lancet, 292, 532-534  https://doi.org/10.1016/S0140-6736(68)92406-9
 
Pineda L., Chwalibog A., Sawosz E., Lauridsen C., Engberg R., Elnif J., Hotowy A., Sawosz F. (2012): Influence of in ovo injection and subsequent provision of silver nanoparticles on growth performance, microbial profile, and immune status of broiler chickens. Open Access Animal Physiology, 4, 1–8.
 
. B. Pradines, . F. Ramiandrasoa, . T. Fusai, . A. Hammadi, . M. Henry, . S. Briolant, . E. Orlandi-Pradines, . H. Bogreau, . J. Mosnier, . E. Baret, . G. Kunesch, . J. Le Bras, . C. Rogier (2005): Generation of Free Radicals and Enhancement of Hemin-induced Membrane Damage by a Catechol Iron Chelator in Plasmodium falciparum. Journal of Biological Sciences, 5, 463-471  https://doi.org/10.3923/jbs.2005.463.471
 
Pyatenko A., Yamaguchi M., Suzuki M. (2007): Synthesis of Spherical Silver Nanoparticles with Controllable Sizes in Aqueous Solutions. Journal of Physical Chemistry C, 111, 7910-7917  https://doi.org/10.1021/jp071080x
 
SALIH A. M., SMITH D. M., PRICE J. F., DAWSON L. E. (1987): Modified Extraction 2-Thiobarbituric Acid Method for Measuring Lipid Oxidation in Poultry. Poultry Science, 66, 1483-1488  https://doi.org/10.3382/ps.0661483
 
Sawosz E., Grodzik M., Zielinska M., Niemiec T., Olszanka B., Chwalibog A. (2009): Nanoparticles of silver do not affect growth, development and DNA oxidative damage in chicken embryos. Archiv für Geflügelkunde, 73, 208–213.
 
Sharma Bechan, Singh Shweta, Siddiqi Nikhat J. (2014): Biomedical Implications of Heavy Metals Induced Imbalances in Redox Systems. BioMed Research International, 2014, 1-26  https://doi.org/10.1155/2014/640754
 
Siwicki A.K., Anderson D.P. (1993): Nonspecific defense mechanisms assay in fish: II. Potential killing activity of neutrophils and macrophages, lysozyme activity in serum and organs and total immunoglobulin (Ig) level in serum. In: Siwicki A.K, Anderson D.P., Waluga J. (eds): Fish Diseases Diagnosis and Prevention Methods. FAO-Project GCP/INT/526/JPN. Inland Fisheries Institute, Olsztyn, Poland, 105–112.
 
Smulikowska S., Rutkowski A. (2005): Nutrient Requirements of Poultry. Feeding Recommendations and Nutritive Value of Feed. The Kielanowski Institute of Animal Physiology and Nutrition of the Polish Academy of Sciences, Jabłonna, Poland. (in Polish)
 
Xu Y., Tang H., Liu J.H., Wang H., Liu Y. (2013): Evaluation of the adjuvant effect of silver nanoparticles both in vitro and in vivo. Toxicology Letters, 12, 42–48.
 
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti