Gene expression and tissue distribution of β-defensins in Chinese Min pigs and Landrace pigs
W. Jiao, Q. Ma, X. Lv, A. Shan, Z. Lihttps://doi.org/10.17221/84/2015-CJASCitation:Jiao W., Ma Q., Lv X., Shan A., Li Z. (2017): Gene expression and tissue distribution of β-defensins in Chinese Min pigs and Landrace pigs. Czech J. Anim. Sci., 62: 178-183.
β-Defensins are a major group of mammalian antimicrobial peptides and play an important role in innate and adaptive immunity due to their antimicrobial, chemotactic, and regulatory activities. In this study, the tissue distribution of porcine β-defensin (pBD-1, pBD-2, and pBD-3) gene expression was determined using real-time polymerase chain reaction in 14-day-old Chinese Min pigs and Landrace pigs. The results showed that in the two breeds, pBD-1 and pBD-2 were primarily expressed in the tongue and kidney, respectively. The pBD-3 genes in Min pigs and Landrace pigs were abundant in the heart and tongue respectively. The mRNA expression levels of the three peptide genes in most tissues were much higher in Min pigs than in Landrace pigs. Collectively, higher defensin gene expressions were observed in some organs and tissues of Min pigs, presumably related to their higher resistance to disease.Keywords:
antimicrobial peptides; swine; mRNA abundanceReferences:
Auvynet Constance, Rosenstein Yvonne (2009): Multifunctional host defense peptides: Antimicrobial peptides, the small yet big players in innate and adaptive immunity. FEBS Journal, 276, 6497-6508 https://doi.org/10.1111/j.1742-4658.2009.07360.xBagnicka E., Strzalkowska N., Jozwik A., Krzyzewski J., Horbanczuk J., Zwierzchowski L. (2010): Expression and polymorphism of defensins in farm animals. Acta Biochimica Polonica, 57, 487–497.Bergman P., Johansson L., Wan H., Jones A., Gallo R. L., Gudmundsson G. H., Hokfelt T., Jonsson A.-B., Agerberth B. (): Induction of the Antimicrobial Peptide CRAMP in the Blood-Brain Barrier and Meninges after Meningococcal Infection. Infection and Immunity, 74, 6982-6991 https://doi.org/10.1128/IAI.01043-06Cederlund Andreas, Gudmundsson Gudmundur H., Agerberth Birgitta (2011): Antimicrobial peptides important in innate immunity. FEBS Journal, 278, 3942-3951 https://doi.org/10.1111/j.1742-4658.2011.08302.xChen Jinyong, Qi Sharina, Guo Rongfu, Yu Bing, Chen Daiwen (2010): Different messenger RNA expression for the antimicrobial peptides β-defensins between Meishan and crossbred pigs. Molecular Biology Reports, 37, 1633-1639 https://doi.org/10.1007/s11033-009-9576-5Dommisch Henrik, Jepsen Søren (2015): Diverse functions of defensins and other antimicrobial peptides in periodontal tissues. Periodontology 2000, 69, 96-110 https://doi.org/10.1111/prd.12093Elahi S., Buchanan R. M., Attah-Poku S., Townsend H. G. G., Babiuk L. A., Gerdts V. (): The Host Defense Peptide Beta-Defensin 1 Confers Protection against Bordetella pertussis in Newborn Piglets. Infection and Immunity, 74, 2338-2352 https://doi.org/10.1128/IAI.74.4.2338-2352.2006Hancock Robert E W, Sahl Hans-Georg (2006): Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies. Nature Biotechnology, 24, 1551-1557 https://doi.org/10.1038/nbt1267Hazlett Linda, Wu Minhao (2011): Defensins in innate immunity. Cell and Tissue Research, 343, 175-188 https://doi.org/10.1007/s00441-010-1022-4Hiratsuka Takeaki, Nakazato Masamitsu, Date Yukari, Mukae Hiroshi, Matsukura Shigeru (2001): Nucleotide Sequence and Expression of Rat β-Defensin-1: Its Significance in Diabetic Rodent Models. Nephron, 88, 65-70 https://doi.org/10.1159/000045961Huttner Kenneth M, Kozak Christine A, Bevins Charles L (1997): The mouse genome encodes a single homolog of the antimicrobial peptide human β-defensin 1. FEBS Letters, 413, 45-49 https://doi.org/10.1016/S0014-5793(97)00875-2Huttner K.M., Brezinski-Caliguri D.J., Mahoney M.M., Diamond G. (1998): Antimicrobial peptide expression is developmentally regulated in the ovine gastrointestinal tract. The Journal of Nutrition, 128, 297–299.Ma Q.Q., Jiao W.J., Wang Z.Y., Wu C.X., Shan A.S., Wang Y.B., Cai J.M. (2014): Tissue specificity and species superiority of cathelicidin gene expression in Chinese indigenous Min pigs. Livestock Science, 161, 36-40 https://doi.org/10.1016/j.livsci.2014.01.004McCormick Thomas S., Weinberg Aaron (2010): Epithelial cell-derived antimicrobial peptides are multifunctional agents that bridge innate and adaptive immunity. Periodontology 2000, 54, 195-206 https://doi.org/10.1111/j.1600-0757.2010.00373.xOppenheim J J (2003): Roles of antimicrobial peptides such as defensins in innate and adaptive immunity. Annals of the Rheumatic Diseases, 62, 17ii-21 https://doi.org/10.1136/ard.62.suppl_2.ii17Qi Sharina, Chen Jinyong, Guo Rongfu, Yu Bing, Chen Daiwen (2009): β-defensins gene expression in tissues of the crossbred and Tibetan pigs. Livestock Science, 123, 161-168 https://doi.org/10.1016/j.livsci.2008.11.009Sang Yongming, Patil Amar A., Zhang Guolong, Ross Chris R., Blecha Frank (2006): Bioinformatic and expression analysis of novel porcine β-defensins. Mammalian Genome, 17, 332-339 https://doi.org/10.1007/s00335-005-0158-0Sang Yongming, Blecha Frank (2009): Porcine host defense peptides: Expanding repertoire and functions. Developmental & Comparative Immunology, 33, 334-343 https://doi.org/10.1016/j.dci.2008.05.006Scott Monisha G., Hancock Robert E. W. (2000): Cationic Antimicrobial Peptides and Their Multifunctional Role in the Immune System. Critical Reviews™ in Immunology, 20, 24- https://doi.org/10.1615/CritRevImmunol.v20.i5.40Selsted Michael E, Ouellette Andre J (2005): Mammalian defensins in the antimicrobial immune response. Nature Immunology, 6, 551-557 https://doi.org/10.1038/ni1206Soman Soja Saghar, Nair Sajith, Issac Aneesh, Arathy D.S., Niyas K.P., Anoop M., Sreekumar E. (2009): Immunomodulation by duck defensin, Apl_AvBD2: In vitro dendritic cell immunoreceptor (DCIR) mRNA suppression, and B- and T-lymphocyte chemotaxis. Molecular Immunology, 46, 3070-3075 https://doi.org/10.1016/j.molimm.2009.06.003Stolzenberg E. D., Anderson G. M., Ackermann M. R., Whitlock R. H., Zasloff M. (1997): Epithelial antibiotic induced in states of disease. Proceedings of the National Academy of Sciences, 94, 8686-8690 https://doi.org/10.1073/pnas.94.16.8686Veldhuizen Edwin J.A., van Dijk Albert, Tersteeg Monique H.G., Kalkhove Stefanie I.C., van der Meulen Jan, Niewold Theo A., Haagsman Henk P. (2007): Expression of β-defensins pBD-1 and pBD-2 along the small intestinal tract of the pig: Lack of upregulation in vivo upon Salmonella typhimurium infection. Molecular Immunology, 44, 276-283 https://doi.org/10.1016/j.molimm.2006.03.005Wang W., Cole A. M., Hong T., Waring A. J., Lehrer R. I. (): Retrocyclin, an Antiretroviral -Defensin, Is a Lectin. The Journal of Immunology, 170, 4708-4716 https://doi.org/10.4049/jimmunol.170.9.4708Xu Z.Y. (1989): Breed Characteristics of Indigenous Pig Breeds in China. Zhejiang Scientific and Technical Press, Hangzhou, China.Yang D. (): -Defensins: Linking Innate and Adaptive Immunity Through Dendritic and T Cell CCR6. Science, 286, 525-528 https://doi.org/10.1126/science.286.5439.525Zhang Guolong, Wu Hua, Shi Jishu, Ganz Tomas, Ross Christopher R, Blecha Frank (1998): Molecular cloning and tissue expression of porcine β-defensin-1. FEBS Letters, 424, 37-40 https://doi.org/10.1016/S0014-5793(98)00134-3