Oxidative stress and antioxidant enzyme defence system in seminal plasma of common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) during spawning season


https://doi.org/10.17221/89/2017-CJASCitation:Shaliutina-Kolešová A., Rui N., Ashtiani S., Rodina M., Cosson j., Linhart O. (2018): Oxidative stress and antioxidant enzyme defence system in seminal plasma of common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) during spawning season  . Czech J. Anim. Sci., 63: 78-84.
download PDF

Assessment of seminal oxidative stress indicators is emerging as a valuable prognostic tool in assisted reproductive technology. We investigated levels of oxidative stress caused by reactive oxygen species (ROS) and the antioxidant enzyme defence system comprising superoxide dismutase (SOD), glutathione reductase (GR), and glutathione peroxidase (GPx) in seminal plasma (SP) of common carp Cyprinus carpio and rainbow trout Oncorhynchus mykiss over the course of the spawning season. Oxidation was determined in lipids and proteins by assessing thiobarbituric acid reactive species (TBARS) and the 2,4-dinitrophenylhydrazine carbonyl groups, respectively. Levels of SOD were assessed by the autoxidation of pyrogallol. Determination of GR and GPx was based on the rate of NADPH oxidation. We observed clear alterations in lipids and proteins over the course of the spawning season in both species. The highest levels of TBARS and CP were recorded late in the season. SOD was not significantly altered in either species. The activity of GR was higher in carp SP late in spawning compared to other times (P < 0.05), while in rainbow trout, GR significantly increased (24.13 ± 2.8 mU/mg protein) in mid-season. A significantly lower GPx activity (9.18 ± 1.32 mU/mg protein) was found in rainbow trout SP early in the spawning season, but no significant differences in GPx were observed over the course of the season in carp. These results provide further understanding of the role of fish SP antioxidants and present new data on the oxidant and antioxidant balance in SP during the spawning season that may be of value in the development of methods for artificial reproduction of teleost species.

Agarwal Ashok, Said Tamer M. (2005): Oxidative stress, DNA damage and apoptosis in male infertility: a clinical approach. BJU International, 95, 503-507  https://doi.org/10.1111/j.1464-410X.2005.05328.x
Aitken R.J. (1999): The human spermatozoon – a cell in crisis? Journal of Reproduction and Fertility, 115, 1–7.
Mohammad Hadi Alavi Sayyed, Psenicka Martin, Rodina Marek, Policar Tomas, Linhart Otomar (2008): Changes of sperm morphology, volume, density and motility and seminal plasma composition in Barbus barbus (Teleostei: Cyprinidae) during the reproductive season. Aquatic Living Resources, 21, 75-80  https://doi.org/10.1051/alr:2008011
Bhattacharjee S. (2010): Sites of generation and physicochemical basis of formation of reactive oxygen species in plant cell. In: Dutta Gupta S. (ed.): Reactive Oxygen Species and Antioxidants in Higher Plants. CRC Press, New York, USA, 1–30.
Bozkurt Y., Ogretmen F., Kokcu O., Ercin U. (2011): Relationship between seminal plasma composition and sperm quality parameters of the Salmo trutta macrostigma (Dumeril, 1858) semen: with emphasis on sperm motility. Czech Journal of Animal Science, 56, 355–364.
Chabory E., Damon C., Lenoir A., Kauselmann G., Kern H., Zevnik B., Garrel C., Saez F., Cadet R., Henry-Berger J., Schoor M., Gottwald U., Habenicht U., Drevet J.R., Vernet P. (2009): Epididymis seleno-independent glutathione peroxidase 5 (GPx5) contributes to the maintenance of sperm DNA integrity. Journal of Clinical Investigation, 119, 2074–2085.
DuTeaux Shelley Brown, Berger Trish, Hess Rex A., Sartini Becky L., Miller Marion G. (2004): Male Reproductive Toxicity of Trichloroethylene: Sperm Protein Oxidation and Decreased Fertilizing Ability1. Biology of Reproduction, 70, 1518-1526  https://doi.org/10.1095/biolreprod.103.022210
Gadea Joaquı́n, Sellés Elena, Marco Marco Antonio, Coy Pilar, Matás Carmen, Romar Raquel, Ruiz Salvador (2004): Decrease in glutathione content in boar sperm after cryopreservation. Theriogenology, 62, 690-701  https://doi.org/10.1016/j.theriogenology.2003.11.013
Garcia M.A., Graham E.F. (1987): Effects of low-molecular-weight fractions (LMWF) from milk, egg yolk, and seminal plasma on freezability of bovine spermatozoa. Cryobiology, 24, 429-436  https://doi.org/10.1016/0011-2240(87)90046-0
Gazo Ievgeniia, Shaliutina-Kolešová Anna, Dietrich Mariola A., Linhartová Pavla, Shaliutina Olena, Cosson Jacky (2015): The effect of reactive oxygen species on motility parameters, DNA integrity, tyrosine phosphorylation and phosphatase activity of common carp ( Cyprinus carpio L.) spermatozoa. Molecular Reproduction and Development, 82, 48-57  https://doi.org/10.1002/mrd.22442
İnanan B.E., Öğretmen F., İnanan T., Yılmaz F. (2016): Total antioxidant capacity, catalase activity, and lipid peroxidation changes in seminal plasma of sex-reversed female and male rainbow trout (Oncorhynchus mykiss) during spawning season. Theriogenology, 86, 1975-1982  https://doi.org/10.1016/j.theriogenology.2016.06.014
Kruger J. C. De W., Smit G. L., Vuren J. H. J., Ferreira J. T. (1984): Some chemical and physical characteristics of the semen of Cyprinus carpio L. and Oreochromis mossambicus (Peters). Journal of Fish Biology, 24, 263-272  https://doi.org/10.1111/j.1095-8649.1984.tb04797.x
Lahnsteiner Franz, Mansour Nabil, Plaetzer Kristjan (2010): Antioxidant systems of brown trout (Salmo trutta f. fario) semen. Animal Reproduction Science, 119, 314-321  https://doi.org/10.1016/j.anireprosci.2010.01.010
Lawrence Richard A., Burk Raymond F. (1976): Glutathione peroxidase activity in selenium-deficient rat liver. Biochemical and Biophysical Research Communications, 71, 952-958  https://doi.org/10.1016/0006-291X(76)90747-6
Lenz Anke-G., Costabel Ulrich, Shaltiel Shmuel, Levine Rodney L. (1989): Determination of carbonyl groups in oxidatively modified proteins by reduction with tritiated sodium borohydride. Analytical Biochemistry, 177, 419-425  https://doi.org/10.1016/0003-2697(89)90077-8
Lenzi A., Gandini L., Lombardo F., Picardo M., Maresca V., Panfili E., Tramer F., Boitani C., Dondero F. (2002): Polyunsaturated fatty acids of germ cell membranes, glutathione and blutathione-dependent enzyme-PHGPx: from basic to clinic. Contraception, 65, 301-304  https://doi.org/10.1016/S0010-7824(02)00276-7
Li Ping, Li Zhi-Hua, Dzyuba Boris, Hulak Martin, Rodina Marek, Linhart Otomar (2010): Evaluating the Impacts of Osmotic and Oxidative Stress on Common Carp (Cyprinus carpio, L.) Sperm Caused by Cryopreservation Techniques1. Biology of Reproduction, 83, 852-858  https://doi.org/10.1095/biolreprod.110.085852
MARKLUND Stefan, MARKLUND Gudrun (1974): Involvement of the Superoxide Anion Radical in the Autoxidation of Pyrogallol and a Convenient Assay for Superoxide Dismutase. European Journal of Biochemistry, 47, 469-474  https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
Marti E., Mara L., Marti J.I., Muiño-Blanco T., Cebrián-Pérez J.A. (2007): Seasonal variations in antioxidant enzyme activity in ram seminal plasma. Theriogenology, 67, 1446-1454  https://doi.org/10.1016/j.theriogenology.2007.03.002
Munkittrick K.R., Moccia R.D. (1987): Seasonal changes in the quality of rainbow trout (Salmo gairdneri) semen: Effect of a delay in stripping on spermatocrit, motility, volume and seminal plasma constituents. Aquaculture, 64, 147-156  https://doi.org/10.1016/0044-8486(87)90350-4
Pasqualotto F., Sharma R., Kobayashi H., Nelson D., Thomas A., Agarwal A. (2001): Oxidative stress in normospermic men undergoing infertility evaluation. Journal of Andrology, 22, 316–322.
Pustowka C., McNiven M A., Richardson G F., Lall S P. (2000): Source of dietary lipid affects sperm plasma membrane integrity and fertility in rainbow trout Oncorhynchus mykiss (Walbaum) after cryopreservation. Aquaculture Research, 31, 297-305  https://doi.org/10.1046/j.1365-2109.2000.00416.x
Schmehl M.K., Anderson S.P., Vazquez I.A., Graham E.F. (1986): The effect of dialysis of extended ram semen prior to freezing on post-thaw survival and fertility. Cryobiology, 23, 406-414  https://doi.org/10.1016/0011-2240(86)90025-8
Schneider Manuela, Förster Heidi, Boersma Auke, Seiler Alexander, Wehnes Helga, Sinowatz Fred, Neumüller Christine, Deutsch Manuel J., Walch Axel, Hrabé de Angelis Martin, Wurst Wolfgang, Ursini Fulvio, Roveri Antonella, Maleszewski Marek, Maiorino Matilde, Conrad Marcus (2009): Mitochondrial glutathione peroxidase 4 disruption causes male infertility. The FASEB Journal, 23, 3233-3242  https://doi.org/10.1096/fj.09-132795
Shaliutina-Kolesova A., Gazo I., Cosson J., Linhart O. (2013): Comparison of oxidant and antioxidant status of seminal plasma and spermatozoa of several fish species. Czech Journal of Animal Science, 58, 313–320.
Shaliutina-Kolešová Anna, Kotas Petr, Štěrba Jan, Rodina Marek, Dzyuba Borys, Cosson Jacky, Linhart Otomar (2016): Protein profile of seminal plasma and functionality of spermatozoa during the reproductive season in the common carp ( Cyprinus carpio ) and rainbow trout ( Oncorhynchus mykiss ). Molecular Reproduction and Development, 83, 968-982  https://doi.org/10.1002/mrd.22737
Souza Carlos Eduardo A., Moura Arlindo A., Monaco Elisa, Killian Gary J. (2008): Binding patterns of bovine seminal plasma proteins A1/A2, 30kDa and osteopontin on ejaculated sperm before and after incubation with isthmic and ampullary oviductal fluid. Animal Reproduction Science, 105, 72-89  https://doi.org/10.1016/j.anireprosci.2007.11.027
Thomson L.K., Fleming S.D., Aitken R.J., De Iuliis G.N., Zieschang J.-A., Clark A.M. (2009): Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis. Human Reproduction, 24, 2061-2070  https://doi.org/10.1093/humrep/dep214
Villemure M., Lazure C., Manjunath P. (2003): Isolation and characterization of gelatin-binding proteins from goat seminal plasma. Reproductive Biology and Endocrinology, 1, 39–48. https://doi.org/10.1186/1477-7827-1-39
Yeni Deniz, Gundogan Mustafa, Hakki Cige I., Avdatek Fatih, Fatih Fida A. (2010): Seasonal Variation of Oxidative Stress Parameters in Ram Seminal Plasma. Journal of Animal and Veterinary Advances, 9, 49-54  https://doi.org/10.3923/javaa.2010.49.54
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti