Study of LGB gene polymorphisms of small ruminants reared in Eastern Europe S., Ilie D.E., Sauer M., Nagy K., Atanasiu T.S., Gavojdian D. (2018): Study of LGB gene polymorphisms of small ruminants reared in Eastern Europe  . Czech J. Anim. Sci., 63: 152-159.
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The objectives of the current research were: (i) to determine the allele and genotype frequencies of the LGB gene in the sheep and goat breeds reared in Eastern Europe; (ii) to implement a comparative study in order to evaluate the milk production potential and efficiency of the main sheep and goat dairy breeds reared in Romania; (iii) to test the genetic basis and feasibility of introducing the molecular-marker assisted selection for this trait into future breeding schemes designed for small ruminants. Totally 731 purebred unrelated sheep (Turcana: 111, Racka: 98, Tsigai: 79, Karakul of Botosani: 60, Transylvanian Merino: 77) and goats (Carpatina: 82, Banat’s White: 73, Saanen: 74, French Alpine: 77) were sampled and genotyped for the earlier detected alleles. The frequencies of A (0.44–0.53) and B (0.42–0.51) alleles of LGB gene were generally equal, while the C allele incidence was significantly lower for all sheep breeds studied. A relatively low or missing incidence of the C allele and no BC genotype were detected in the studied sheep breeds. All studied sheep breeds were in Hardy–Weinberg equilibrium. Gene homozygosity was lower than gene heterozygosity, with the effective allele numbers ranging from 2.00 (Transylvanian Merino) to 2.23 (Tsigai). Two alleles (A and B) and two genotypes (AA and AB) were detected in the four goat breeds studied. Allele A was the most frequent allele found in all breeds (0.57–0.68), while AB genotype had the highest frequency. Gene homozygosity was higher than gene heterozygosity, while the effective allele numbers varied between 1.76 (Banat’s White) and 1.96 (Carpatina). The frequency of alleles and genotypes was similar to that reported in other Eastern sheep and goat breeds, however polymorphism has not been studied yet among these breeds in such a high number in Romania. An association study between single nucleotide polymorphisms and milk production traits should follow.

Amigo Lourdes, Recio Isidra, Ramos Mercedes (2000): Genetic polymorphism of ovine milk proteins: its influence on technological properties of milk — a review. International Dairy Journal, 10, 135-149
Anton I., Zsolnai A., Fesus L., Kukovics S., Molnar A. (1999): Survey of β-lactoglobulin and αs1-casein polymorphism in Hungarian dairy sheep breeds and crosses at DNA level. Archiv fur Tierzucht, 42, 387–392.
Balthazar C.F., Pimentel T.C., Ferrão L.L., Almada C.N., Santillo A., Albenzio M., Mollakhalili N., Mortazavian A.M., Nascimento J.S., Silva M.C., Freitas M.Q., Sant’Ana A.S., Granato D., Cruz A.G. (2017): Sheep Milk: Physicochemical Characteristics and Relevance for Functional Food Development. Comprehensive Reviews in Food Science and Food Safety, 16, 247-262
Cubrik-Curik V., Feligini M., Lukac-Havranek J., Curik I., Enne G. (2002): Genetic polymorphism of β-lactoglobulin in native sheep from the Island of Pag. Food Technology and Biotechnology, 40, 75–78.
El Hanafy A.A.M., Qureshi M.I., Sabir J., Mutawakil M., Ahmed M.M.M., El Ashmaoui H., Ramadan H.A.M.I., Abou-Alsoud M., Sadek M.A. (2016): Nucleotide sequencing and DNA polymorphism studies of beta-lactoglobulin gene in native Saudi goat breeds in relation to milk yield. Czech Journal of Animal Science, 60, 132-138
Elmaci Cengiz, Oner Yasemin, Balcioglu M. Soner (2006): Genetic Polymorphism of β-Lactoglobulin Gene in Native Turkish Sheep Breeds. Biochemical Genetics, 44, 376-381
Elyasi G., Shodja J., Nassiry M.R., Tahmasebi A., Pirahary O., Javanmard A. (2010): Polymorphism of β-Lactoglobulin Gene in Iranian Sheep Breeds Using PCR-RFLP. Journal of Molecular Genetics, 2, 6-9
ERHARDT G. (1989): Evidence for a third allele at the β-lactoglobulin (β-Lg) locus of sheep milk and its occurrence in different breeds. Animal Genetics, 20, 197-204
FAO/IAEA (2004): Agriculture Biotechnology Laboratory – Handbook of Laboratory Exercises. IAEA Laboratories, Seibersdorf, Austria.
Feligini M., Parma P., Aleandri R., Greppi G.F., Enne G. (1998): PCR-RFLP test for direct determination of β-lactoglobulin genotype in sheep. Animal Genetics, 29, 460–477.
Gavojdian Dinu, Cziszter Ludovic-Toma, Sossidou Evangelia, Pacala Nicolae (2013): Improving performance of Zackel sheep through cross-breeding with prolific Bluefaced Leicester under semi-intensive and extensive production systems. Journal of Applied Animal Research, 41, 432-441
Georgescu S.E., Ene A., Dudu A., Ghita E., Costache M. (2016): Genetic polymorphisms of β-lactoglobulin and α-s1-casein genes in Romanian Racka sheep. Scientific Papers Animal Science and Biotechnologies, 49, 50–53.
Hill T., Lewicki P. (2007): Statistics: Methods and Applications. StatSoft, Tulsa, USA.
Kawecka Aldona, Radko Anna (2011): Genetic polymorphism of β-lactoglobulin in sheep raised for milk production. Journal of Applied Animal Research, 39, 68-71
Korkmaz Agaoglu O., Cinar Kul B., Akyuz B., Elmaz O., Ozcelik Metin M., Saatci M., Ertugrul O. (2012): Identification of β-lactoglobulin gene SacII polymorphism in Honamli, Hair and Saanen goat breeds reared in Burdur vicinity. Kafkas Universitesi Veteriner Fakultesi Dergisi, 18, 385–388.
Kusza Szilvia, Sziszkosz Nikolett, Nagy Krisztina, Masala Amela, Kukovics Sándor, András Jávor (2015): Preliminary result of a genetic polymorphism of β-lactoglobulin gene and the phylogenetic study of ten balkan and central european indigenous sheep breeds. Acta Biochimica Polonica, 62, 109-112
Leeds T. D., Notter D. R., Leymaster K. A., Mousel M. R., Lewis G. S. (2012): Evaluation of Columbia, USMARC-Composite, Suffolk, and Texel rams as terminal sires in an extensive rangeland production system: I. Ewe productivity and crossbred lamb survival and preweaning growth1,2. Journal of Animal Science, 90, 2931-2940
Macha J., Novackova I. (1974): Genetic polymorphism of beta-lactoglobulin in sheep’s milk. Živočišná Výroba, 19, 883–888. (in Czech)
Mohammadi A., Nassiry M.R., Elyasi G., Shodja J. (2006): Genetic polymorphism of β-lactoglobulin in certain Iranian and Russian sheep breeds. Iranian Journal of Biotechnology, 4, 265–268.
Moioli B, Pilla F, Tripaldi C (1998): Detection of milk protein genetic polymorphisms in order to improve dairy traits in sheep and goats: a review. Small Ruminant Research, 27, 185-195
Mroczkowski S., Korman K., Erhardt G., Piwczynski D., Borys B. (2004): Sheep milk protein polymorphism and its effect on milk performance of Polish Merino. Archiv fur Tierzucht, 47, 114–121.
Palhiere I., Larroque H., Clement V., Tosser-Klopp G., Rupp R. (2014): Genetic parameters and QTL detection for milking speed in dairy Alpine and Saanen goats. In: Proc. 10th World Congress of Genetics Applied to Livestock Production, Vancouver, Canada, 892.
Park Y.W., Juárez M., Ramos M., Haenlein G.F.W. (2007): Physico-chemical characteristics of goat and sheep milk. Small Ruminant Research, 68, 88-113
PENA RAMONA N., SÁNCHEZ ARMAND, FOLCH JOSEP M. (): Characterization of genetic polymorphism in the goat β-lactoglobulin gene. Journal of Dairy Research, 67, 217-224
Ramos A.M., Matos C.A.P., Russo-Almeida P.A., Bettencourt C.M.V., Matos J., Martins A., Pinheiro C., Rangel-Figueiredo T. (2009): Candidate genes for milk production traits in Portuguese dairy sheep. Small Ruminant Research, 82, 117-121
Selvaggi Maria (2015): β-Lactoglobulin Gene Polymorphisms in Sheep and Effects on Milk Production Traits: A Review. Advances in Animal and Veterinary Sciences, 3, 478-484
Sossidou E., Ligda C., Mastranestasis I., Tsiokos D., Samartzi F. (2013): Sheep and goat farming in Greece: implications and challenges for the sustainable development of less favoured areas. Scientific Papers Animal Science and Biotechnologies, 46, 446–449.
Todaro M., Dattena M., Acciaioli A., Bonanno A., Bruni G., Caroprese M., Mele M., Sevi A., Marinucci M. Trabalza (2015): Aseasonal sheep and goat milk production in the Mediterranean area: Physiological and technical insights. Small Ruminant Research, 126, 59-66
Yeh F.C., Yong R. (1999): POPGENE Version 1.31: Microsoft-based Freeware for Population Genetic Analysis. University of Alberta, Edmonton, Canada.
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