Effects of dietary conjugated linoleic acid on lipid peroxidation in breast and thigh muscles of broiler chickens

https://doi.org/10.17221/95/2016-CJASCitation:Liu Y., Tang G., Yang J., Li W. (2017): Effects of dietary conjugated linoleic acid on lipid peroxidation in breast and thigh muscles of broiler chickens. Czech J. Anim. Sci., 62: 331-338.
download PDF
The effect of dietary supplementation with conjugated linoleic acid (CLA) on lipid peroxidation in the breast muscles (Pectoralis major and Pectoralis minor) and thigh muscles of broiler chickens was investigated. A total of ninety-six 21-day-old Arbor Acres male broiler chickens were assigned to 2 dietary treatments (0 and 1.5% CLA) with 8 replicates per 6 chickens each. The left breast and thigh muscles of broiler chickens were excised on day 42. The broilers receiving the CLA diet exhibited lower malondialdehyde content and reactive oxygen species production (P < 0.01) in the breast and thigh muscles than the broilers receiving the control diet. After the dietary CLA supplementation, the glutathione (GSH) content in the breast and thigh muscles of the broiler chickens fed the CLA diet increased by 21.89 and 21.56%, respectively (P < 0.05) while the γ-glutamylcysteine synthetase(γ-GCS)activity increased by 28.57 and 25.80% (P < 0.05), respectively. Dietary CLA significantly increased the CLA content (P < 0.01) and saturated fatty acid content (P < 0.05), and decreased the monounsaturated fatty acid content (P < 0.01) in the breast and thigh muscles. These results showed that dietary CLA may decrease the lipid peroxidation level in the breast and thigh muscles of broiler chickens perhaps through increasing the γ-GCS activity to induce GSH synthesis and changing the fatty acid composition to increase oxidative stability.
References:
Aebi H. (1984): Catalase in vitro. Methods in Enzymology, 105, 121–126.
 
Aletor V., Eder K., Becker K., Paulicks B., Roth F., Roth-Maier D. (2003): The effects of conjugated linoleic acids or an alpha-glucosidase inhibitor on tissue lipid concentrations and fatty acid composition of broiler chicks fed a low-protein diet. Poultry Science, 82, 796-804 https://doi.org/10.1093/ps/82.5.796
 
Bölükbaşi Ş.C. (2006): Effect of dietary conjugated linoleic acid (CLA) on broiler performance, serum lipoprotein content, muscle fatty acid composition and meat quality during refrigerated storage. British Poultry Science, 47, 470-476 https://doi.org/10.1080/00071660600827716
 
Cao Y.L., Guo P., Xu Y.C., Zhao B. (2005): Simultaneous detection of NO and ROS by ESR biological systems. Methods in Enzymology, 396, 77–83.
 
Chae S.H., Keeton J.T., Smith S.B. (2004): Conjugated Linoleic Acid Reduces Lipid Oxidation in Aerobically Stored, Cooked Ground Beef Patties. Journal of Food Science, 69, S306-S309 https://doi.org/10.1111/j.1365-2621.2004.tb09895.x
 
Chamruspollert M., Sell J. (1999): Transfer of dietary conjugated linoleic acid to egg yolks of chickens. Poultry Science, 78, 1138-1150 https://doi.org/10.1093/ps/78.8.1138
 
Chin S.F., Liu W., Storkson J.M., Ha Y.L., Pariza M.W. (1992): Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. Journal of Food Composition and Analysis, 5, 185-197 https://doi.org/10.1016/0889-1575(92)90037-K
 
Corino C, Mourot J, Magni S, Pastorelli G, Rosi F (2002): Influence of dietary conjugated linoleic acid on growth, meat quality, lipogenesis, plasma leptin and physiological variables of lipid metabolism in rabbits.. Journal of Animal Science, 80, 1020- https://doi.org/10.2527/2002.8041020x
 
Corino C., Magni S., Pastorelli G., Rossi R., Mourot J. (2003): Effect of conjugated linoleic acid on meat quality, lipid metabolism, and sensory characteristics of dry-cured hams from heavy pigs. Journal of Animal Science, 81, 2219- https://doi.org/10.2527/2003.8192219x
 
Du M., Ahn D. U. (2002): Effect of Dietary Conjugated Linoleic Acid on the Growth Rate of Live Birds and on the Abdominal Fat Content and Quality of Broiler Meat. Poultry Science, 81, 428-433 https://doi.org/10.1093/ps/81.3.428
 
Du M., Ahn D. U., Nam K. C., Sell J. L. (2001): Volatile Profiles and Lipid Oxidation of Irradiated Cooked Chicken Meat from Laying Hens Fed Diets Containing Conjugated Linoleic Acid. Poultry Science, 80, 235-241 https://doi.org/10.1093/ps/80.2.235
 
Igarashi M., Miyazawa T. (2001): The growth inhibitory effect of conjugated linoleic acid on a human hepatoma cell line, HepG2, is induced by a change in fatty acid metabolism, but not the facilitation of lipid peroxidation in the cells. Biochimica et Biophysica Acta: Molecular and Cell Biology of Lipids, 1530, 162–171.
 
KAWAHARA Satoshi, TAKENOYAMA Shin-ichi, TAKUMA Kazuaki, MUGURUMA Michio, YAMAUCHI Kiyoshi (2009): Effects of dietary supplementation with conjugated linoleic acid on fatty acid composition and lipid oxidation in chicken breast meat. Animal Science Journal, 80, 468-474 https://doi.org/10.1111/j.1740-0929.2009.00658.x
 
KIM Hye-Kyeong, KIM Sung-Ran, AHN Ji-Yoon, CHO Il-Jin, YOON Chil-Suk, HA Tae-Yowl (2005): Dietary Conjugated Linoleic Acid Reduces Lipid Peroxidation by Increasing Oxidative Stability in Rats. Journal of Nutritional Science and Vitaminology, 51, 8-15 https://doi.org/10.3177/jnsv.51.8
 
Ko Y. H., Yang H. Y., Jang I. S (2004): Effect of Conjugated Linoleic Acid on Intestinal and Hepatic Antioxidant Enzyme Activity and Lipid Peroxidation in Broiler Chickens. Asian-Australasian Journal of Animal Sciences, 17, 1162-1167 https://doi.org/10.5713/ajas.2004.1162
 
Kumari Ramiah Suriya, Meng Goh Yong, Ebrahimi Mahdi (2014): Dietary Conjugated Linoleic Acid Alters Oxidative Stability and Alleviates Plasma Cholesterol Content in Meat of Broiler Chickens. The Scientific World Journal, 2014, 1-10 https://doi.org/10.1155/2014/949324
 
Lawrence Richard A., Burk Raymond F. (1976): Glutathione peroxidase activity in selenium-deficient rat liver. Biochemical and Biophysical Research Communications, 71, 952-958 https://doi.org/10.1016/0006-291X(76)90747-6
 
Lin H., Boylston D., Chang M.J., Luedecke L.O., Shultz T.D. (1995): Survey of the Conjugated Linoleic Acid Contents of Dairy Products. Journal of Dairy Science, 78, 2358-2365 https://doi.org/10.3168/jds.S0022-0302(95)76863-1
 
Min B., Ahn D.U. (2005): Mechanism of lipid peroxidation in meat and meat products –a review. Food Science and Biotechnology, 14, 152–163.
 
Narciso-Gaytan C., Shin D., Sams A. R., Keeton J. T., Miller R. K., Smith S. B., Sanchez-Plata M. X. (): Lipid oxidation stability of omega-3- and conjugated linoleic acid-enriched sous vide chicken meat. Poultry Science, 90, 473-480 https://doi.org/10.3382/ps.2010-01002
 
Palacios A., Piergiacomi V., Catata A. (2003): Antioxidant effect of conjugated linoleic acid and vitamin A during nonenzymatic lipid peroxidation of rat liver microsomes and mitochondria. Molecular Cellular Biochemistry, 250, 107–113. https://doi.org/10.1023/A:1024977613141
 
Pirozhkov Sergei V., Eskelson Cleamond D., Watson Ronald R., Hunter Glen C., Piotrowski Joseph J., Bernhard Victor (1992): Effect of chronic consumption of ethanol and vitamin E on fatty acid composition and lipid peroxidation in rat heart tissue. Alcohol, 9, 329-334 https://doi.org/10.1016/0741-8329(92)90076-M
 
Saha Siddhartha S., Ghosh Mahua (2011): Antioxidant effect of vegetable oils containing conjugated linolenic acid isomers against induced tissue lipid peroxidation and inflammation in rat model. Chemico-Biological Interactions, 190, 109-120 https://doi.org/10.1016/j.cbi.2011.02.030
 
Sirri F., Tallarico N., Meluzzi A., Franchini A. (2003): Fatty acid composition and productive traits of broiler fed diets containing conjugated linoleic acid. Poultry Science, 82, 1356-1361 https://doi.org/10.1093/ps/82.8.1356
 
Spitz Douglas R., Oberley Larry W. (1989): An assay for superoxide dismutase activity in mammalian tissue homogenates. Analytical Biochemistry, 179, 8-18 https://doi.org/10.1016/0003-2697(89)90192-9
 
Sukhija Pritam S., Palmquist D. L. (1988): Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. Journal of Agricultural and Food Chemistry, 36, 1202-1206 https://doi.org/10.1021/jf00084a019
 
Suksombat W., Boonmee T., Lounglawan P. (2007): Effects of Various Levels of Conjugated Linoleic Acid Supplementation on Fatty Acid Content and Carcass Composition of Broilers. Poultry Science, 86, 318-324 https://doi.org/10.1093/ps/86.2.318
 
Szymczyk Beata, Pisulewski Paweł M., Szczurek Witold, Hanczakowski Piotr (2001): Effects of conjugated linoleic acid on growth performance, feed conversion efficiency, and subsequent carcass quality in broiler chickens. British Journal of Nutrition, 85, 465- https://doi.org/10.1079/BJN2000293
 
Wiegand B R, Sparks J C, Parrish F C, Zimmerman D R (2002): Duration of feeding conjugated linoleic acid influences growth performance, carcass traits, and meat quality of finishing barrows.. Journal of Animal Science, 80, 637- https://doi.org/10.2527/2002.803637x
 
Zhang H.J., Guo Y.M., Tian Y.D., Yuan J.M. (2008): Dietary conjugated linoleic acid improves antioxidant capacity in broiler chicks. British Poultry Science, 49, 213-221 https://doi.org/10.1080/00071660801989836
 
download PDF

© 2019 Czech Academy of Agricultural Sciences