Blood biochemical parameters measured during theperiparturient period incows of Holstein and Fleckvieh breeds differing inproduction purposeŠtolcová M., Řehák D., Bartoň L., Rajmon R. (2020): Blood biochemical parameters measured during the periparturient period in cows of Holstein and Fleckvieh breeds differing in production purpose. Czech J. Anim. Sci., 65: 172-181.
download PDF

The aim of this study was to compare the metabolic status of dairy and dual-purpose cows kept in a single herd under identical management conditions. Milk yield and blood biochemical parameters were examined during the periparturient period in Holstein (H) and Fleckvieh (F) cows. Blood samples were first taken on average 14 days prior to the expected date of calving, next in the peripartal period (1–4 days postpartum), and then at weekly intervals (together with milk samples) until the end of the experiment (8 weeks postpartum). Milk yields were higher in H cows from the second week after calving whereas milk protein content always was higher in F cows over the whole experimental period. The level of metabolic stress determined using blood concentrations of nonesterified fatty acids (NEFA), β-hydroxybutyrate (BHB), triglycerides, and cholesterol was similar in both breeds. The proportion of animals with concentrations of NEFA and BHB above thresholds indicating increased risk of negative energy balance and subclinical ketosis was higher in the H breed in the first 2 weeks after calving. This corresponds with numerically higher concentrations of NEFA and BHB in H cows. Changes in the protein status of animals generally reflected the development in energy metabolism parameters. Serum total protein, albumin,and urea levels were similar in the two breeds. Total globulin was higher in H cows than in F cows in weeks 3, 4, and 5 after calving, and the albumin-to-globulin ratio was lower in H cows than in F cows in weeks 2, 3, and 4 after calving. In conclusion, although the onset of changes in key metabolic parameters was rather faster and more pronounced in the H breed, similar dynamics in the development of these parameters indicated similar levels of adaptive performance and body energy mobilization processes in the two breeds.

Alberghina D, Giannetto C, Vazzana I, Ferrantelli V, Piccione G. Reference intervals for total protein concentration, serum protein fractions, and albumin/globulin ratios in clinically healthy dairy cows. J Vet Diagn Invest. 2011 Jan;23(1):111-4.
Andersen JB, Friggens NC, Larsen T, Vestergaard M, Ingvartsen KL. Effect of energy density in the diet and milking frequency on plasma metabolites and hormones in early lactation dairy cows. J Vet Med Ser A. 2004 May;51(2):52-7.
Barth K, Aulrich K, Haufe HC, Muller U, Schaub D, Schulz F. Metabolic status in early lactating dairy cows of two breeds kept under conditions of organic farming – A case study. Landbauforschung. 2011;61:307-16.
Bobbo T, Fiore E, Gianesella M, Morgante M, Gallo L, Ruegg PL, Bittante G, Cecchinato A. Variation in blood serum proteins and association with somatic cell count in dairy cattle from multi-breed herds. Animal. 2017 Dec;11(12):2309-19.
Cozzi G, Ravarotto L, Gottardo F, Stefani AL, Contiero B, Moro L, Brscic M, Dalvit P. Short communication: Reference values for blood parameters in Holstein dairy cows: Effect of parity, stage of lactation and season of production. J Dairy Sci. 2011 Aug;94(8):3895-901.
Danowski K, Sorg D, Gross J, Meyer HHD, Kliem H. Innate defense capability of challenged primary bovine mammary epithelial cells after an induced negative energy balance in vivo. Czech J Anim Sci. 2012 May;57(5):207-19.
Drackley JK, Overton TR, Douglas GN. Adaptations of glucose and long-chain fatty acids metabolism in liver of dairy cows during the periparturient period. J Dairy Sci. 2001 Jun;84(E-Suppl.):100-12.
Duffield TF, Lissemore KD, McBride BW, Leslie KE. Impact of hyperketonemia in early lactation dairy cows on health and production. J Dairy Sci. 2009 Feb;92(2):571-80.
Forsback L, Lindmark-Mansson H, Andren A, Akerstedt M, Andree L, Svennersten-Sjaunja K. Day-to-day variation in milk yield and milk composition at the udder-quarter level. J Dairy Sci. 2010 Aug;93(8):3569-77.
Gross JJ, Schwarz FJ, Eder K, Van Dorland HA, Bruckmaier RM. Liver fat content and lipid metabolism in dairy cows during early lactation and during a mid-lactation feed restriction. J Dairy Sci. 2013 Aug;96(8):5008-17.
Gross JJ, Kessler EC, Albrecht C, Bruckmaier RM. Response of the cholesterol metabolism to a negative energy balance in dairy cows depends on the lactational stage. PLoS One. 2015 Jun;10(6):e0121956.
ICAR. Milk recording surveys on cow, sheep and goats [Internet]. 2019 [cited 2019 December 13]. Available from
Jozwik A, Strzalkowska N, Bagnicka E, Grzybek W, Krzyzewski J, Polawska E, Kolataj A, Horbanczuk JO. Relationship between milk yield, stage of lactation, and some blood serum metabolic parameters of dairy cows. Czech J Anim Sci. 2012 Jan;57(8):353-60.
Kessel A. Bovine haematology and biochemistry. In: Cockcroft P, editor. Bovine medicine. Hoboken, NJ-USA: Wiley Blackwell; 2015. p. 146-60.
Littel RC, Pendergast J, Natarajan R. Modelling covariance structure in the analysis of repeated measures data. Stat Med. 2000 Jul;19(13):1793-819.<1793::AID-SIM482>3.0.CO;2-Q
McArt JAA, Nydam DV, Oetzel GR. Epidemiology of subclinical ketosis in early lactation in dairy cattle. J Dairy Sci. 2012 Sep;95(9):5056-66.
McArt JAA, Nydam DV, Oetzel GR, Overton TR, Ospina PA. Elevated non-esterified fatty acids and β-hydroxy-butyrate and their association with transition dairy cow performance. Vet J. 2013 Dec;198(3):560-70.
Oregon State University. Biochemistry reference interval [Internet]. 2019 [cited 2019 June 6]. Available from
Park AF, Shirley JE, Titgemeyer EC, Cochran RC, DeFrain JM, Wickersham EE, Johnson DE. Characterization of plasma metabolites in Holstein dairy cows during the periparturient period. Int J Dairy Sci. 2010 Apr;5(4):253-63.
Pennsylvania State University. Metabolic profiling. Reference values [Internet]. 2013 [cited 2019 June 6]. Available from
Puppel K, Kuczynska B. Metabolic profiles of cow’s blood: A review. J Sci Food Agric. 2016 Oct;96(13):4321-8.
Pysera B, Opalka A. The effect of gestation of dairy cows on lipid and lipoprotein patterns and composition in serum during winter and summer feeding. J Anim Feed Sci. 2000 Jul;9(3):411-24.
Quiroz-Rocha GF, Leblanc SJ, Duffield TF, Wood D, Leslie KE, Jacobs RM. Reference limits for biochemical and hematological analysts of dairy cows one week before and one week after parturition. Can Vet J. 2009 Apr;50(4):383-8.
Raggio G, Lobley GE, Berthiaume R, Pellerin D, Allard G, Dubreuil P, Lapierre H. Effect of protein supply on hepatic synthesis of plasma and constitutive proteins in lactating dairy cows. J Dairy Sci. 2007 Jan;90(1):352-9.
Roche JR, Bell AW, Overton TR, Loor JJ. Nutritional management of the transition cow in the 21st century – A paradigm shift in thinking. Anim Prod Sci. 2013 Jan;53(9):1000-23.
Sevinc M, Basoglu A, Birdane FM, Boydak M. Liver function in dairy cows with fatty liver. Rev Med Vet. 2001;152(4):297-300.
Tothova C, Nagy O, Kovac G. Serum proteins and their diagnostic utility in veterinary medicine: A review. Vet Med-Czech. 2016 Sep;61(9):475-96.
Urdl M, Gruber L, Obritzhauser W, Schauer A. Metabolic parameters and their relationship to energy balance in multiparous Simmental, Brown Swiss and Holstein cows in the periparturient period as influenced by energy supply pre- and post-calving. J Anim Physiol Anim Nutr. 2015 Feb;99(1):174-89.
Van Saun RJ. Indicators of dairy cow transition risks: Metabolic profiling revisited. Tierarztl Prax Ausg G. 2016 Feb;44(2):118-26.
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti