A novel porcine gene, USP7, differentially expressed in the musculus longissimus from Wujin and Large White pigs
Y.G. Liu, S.Z. Gaohttps://doi.org/10.17221/1709-CJASCitation:Liu Y.G., Gao S.Z. (2010): A novel porcine gene, USP7, differentially expressed in the musculus longissimus from Wujin and Large White pigs. Czech J. Anim. Sci., 55: 37-41.
The mRNA differential display technique was performed to investigate differences in the gene expression in the musculus longissimus from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete cDNA sequence was obtained using the rapid amplification of cDNA ends (RACE) method. The sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 1 103 amino acids, which is homologous with the ubiquitin specific peptidase 7 (USP7) of four species, rat (identity 98%), human (98%), mouse (98%) and chicken (95%), so it can be defined as the porcine USP7 gene. The differences in the USP7 gene expression in muscles from Wujin and Large White pigs were confirmed using semi-quantitative RT-PCR. The gene expression analysis in eight tissues of a Wujin × Large White cross showed that USP7 was expressed in all the tissues, except for fat.
pig; mRNA differential display; RACE; USP7 gene