Optimal inclusion level of butylated hydroxytoluene in semen extender improves the quality of post-thawed canine sperm

https://doi.org/10.17221/6273-CJASCitation:Ziaullah M., Ijaz A., Aleem M., Mahmood A.K., Rahman H., Bhatti S., Farooq U., Sohail M.U. (2012):  Optimal inclusion level of butylated hydroxytoluene in semen extender improves the quality of post-thawed canine sperm. Czech J. Anim. Sci., 57: 377-381.
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The study was conducted to evaluate the potential cryoprotective effect of butylated hydroxytoluene (BHT) through post-thaw evaluation of canine semen and its optimal inclusion level. Ejaculated canine semen was extended in TRIS-glucose egg yolk extender containing various concentrations of BHT (0.5, 1.0, 1.5, 2.0, and 2.5mM). Semen was frozen at −196°C using 200 × 106 spermatozoa per 0.5 ml straws and post-thaw evaluation was carried out in terms of sperm motility, viability, plasma membrane integrity, and acrosomal integrity through phase-contrast microscope, supravital staining, hypo-osmotic swelling test, and normal acrosomal ridge, respectively. BHT was found to improve (P > 0.005) all post-thawed semen quality parameters at an inclusion level of 1.0mM in the extended semen. However, higher concentrations than this were found to have detrimental effects.  

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