Polymorphism identification in goat DGAT1 and STAT5A genes and association with milk production traits

https://doi.org/10.17221/6862-CJASCitation:An X.P., Hou J.X., Zhao H.B., Bai L., Peng J.Y., Zhu C.M., Yan Q.M., Song Y.X., Wang J.G., Cao B.Y. (2013): Polymorphism identification in goat DGAT1 and STAT5A genes and association with milk production traits. Czech J. Anim. Sci., 58: 321-327.
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Polymorphisms of DGAT1 and STAT5A genes in Xinong Saanen and Guanzhong goat breeds were investigated. PCR-RFLP, SSCP, and DNA sequencing were used to identify three SNPs: DQ380250:g.407_408insC in the DGAT1 gene, AJ237937:g.6798C>T and g.6852C>T in the STAT5A gene. In DGAT1 g.407_408insC locus, the frequencies of C allele were 0.79–0.85, and frequencies of C+ allele were 0.21–0.15. At STAT5A g.6852C>T locus, frequencies of C allele were 0.70–0.72, and frequencies of T allele were 0.30–0.28. Compared with goats with DGAT1 CC, those with CC+ genotype had greater milk fat (P < 0.05). The goats with STAT5A CT had greater milk yield than those with CC genotype (P < 0.05). The results showed that does with CCCT and CC+CT yielded more milk than those with CCCC(P < 0.05). In addition, does with CC+CT had the highest milk fat in comparison with other combination genotypes (P < 0.05).  
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