Association of a synonymous mutation of the PGAM2 gene and growth traits in rabbits
Z.-L. Wu, S.-Y. Chen, X.-B. Jia, S.-J. Laihttps://doi.org/10.17221/8079-CJASCitation:Wu Z.-., Chen S.-., Jia X.-., Lai S.-. (2015): Association of a synonymous mutation of the PGAM2 gene and growth traits in rabbits. Czech J. Anim. Sci., 60: 139-144.
Phosphoglycerate mutase (PGAM2) catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate and releases energy during glycolysis in muscle tissues. PGAM2 has been considered as a candidate gene to influence growth, development, and carcass traits in livestock. The aim of this study was to investigate the association between polymorphisms of PGAM2 and growth traits in rabbits. Three single nucleotide polymorphisms (SNPs) were identified by direct sequencing in 20 random individuals from three breeds, including c.-10C>T, c.195C>T, and c.414+17C>T. The c.195C>T was genotyped by PCR-RFLP in a total of 222 rabbits of three breeds (Tianfu black, 53 animals; Ira, 91 animals; Champagne, 78 animals). The average allele frequency among the breeds was 0.52 for allele T and 0.48 for C. The heterozygosity and effective number of alleles were 0.4992 and 1.996, respectively. The association results revealed the CT genotype of c.195C>T was associated significantly (P < 0.05) with greater body weight at 84 days of age (BW84) and with average daily weight gain (ADG). However, association of the genotypes with other production traits was not observed. The results of this study suggested PGAM2 is one of the candidate genes affecting BW84 and ADG in the rabbit.Keywords:SNPs; body weight; average daily weight gain; association analysis; candidate geneReferences:
Blasco A., Ouhayoun J., Masoero G. (1993): Harmonization of criteria and terminology in rabbit meat research. Journal of Applied Rabbit Research, 15, 64.Davoli R., Fontanesi L., Zambonelli P., Bigi D., Gellin J., Yerle M., Milc J., Braglia S., Cenci V., Cagnazzo M., Russo V. (2002): Isolation of porcine expressed sequence tags for the construction of a first genomic transcript map of the skeletal muscle in pig. Animal Genetics, 33, 3-18 https://doi.org/10.1046/j.1365-2052.2002.00800.xDunner S., Sevane N., García D., Cortés O., Valentini A., Williams J.L., Mangin B., Cañón J., Levéziel H. (2013): Association of genes involved in carcass and meat quality traits in 15 European bovine breeds. Livestock Science, 154, 34-44 https://doi.org/10.1016/j.livsci.2013.02.020Fontanesi L., Davoli R., Nanni Costa L., Scotti E., Russo V. (2003): Study of candidate genes for glycolytic potential of porcine skeletal muscle: identification and analysis of mutations, linkage and physical mapping and association with meat quality traits in pigs. Cytogenetic and Genome Research, 102, 145-151 https://doi.org/10.1159/000075740Fontanesi L., Davoli R., Nanni Costa L., Beretti F., Scotti E., Tazzoli M., Tassone F., Colombo M., Buttazzoni L., Russo V. (2008): Investigation of candidate genes for glycolytic potential of porcine skeletal muscle: Association with meat quality and production traits in Italian Large White pigs. Meat Science, 80, 780-787 https://doi.org/10.1016/j.meatsci.2008.03.022Fontanesi L., Dall'Olio S., Spaccapaniccia E., Scotti E., Fornasini D., Frabetti A., Russo V. (2012): A single nucleotide polymorphism in the rabbit growth hormone (GH1) gene is associated with market weight in a commercial rabbit population. Livestock Science, 147, 84-88 https://doi.org/10.1016/j.livsci.2012.04.006Geldermann H., Muller E., Moser G., Reiner G., Bartenschlager H., Cepica S., Stratil A., Kuryl J., Moran C., Davoli R., Brunsch C. (2003): Genome-wide linkage and QTL mapping in porcine F2 families generated from Pietrain, Meishan and Wild Boar crosses. Journal of Animal Breeding and Genetics, 120, 363-393 https://doi.org/10.1046/j.0931-2668.2003.00408.xJohnsen Ulrike, Schönheit Peter (2007): Characterization of cofactor-dependent and cofactor-independent phosphoglycerate mutases from Archaea. Extremophiles, 11, 647-657 https://doi.org/10.1007/s00792-007-0094-xMarchler-Bauer A., Lu S., Anderson J. B., Chitsaz F., Derbyshire M. K., DeWeese-Scott C., Fong J. H., Geer L. Y., Geer R. C., Gonzales N. R., Gwadz M., Hurwitz D. I., Jackson J. D., Ke Z., Lanczycki C. J., Lu F., Marchler G. H., Mullokandov M., Omelchenko M. V., Robertson C. L., Song J. S., Thanki N., Yamashita R. A., Zhang D., Zhang N., Zheng C., Bryant S. H. (): CDD: a Conserved Domain Database for the functional annotation of proteins. Nucleic Acids Research, 39, D225-D229 https://doi.org/10.1093/nar/gkq1189Mikhailik Anatoly, Ford Bradley, Keller James, Chen Yunting, Nassar Nicolas, Carpino Nick (2007): A Phosphatase Activity of Sts-1 Contributes to the Suppression of TCR Signaling. Molecular Cell, 27, 486-497 https://doi.org/10.1016/j.molcel.2007.06.015Nei M., Roychoudhury A. (1974): Sampling variances of heterozygosity and genetic distance. Genetics, 76, 379–390.Orrù L., Catillo G., Napolitano F., De Matteis G., Scatà M.C., Signorelli F., Moioli B. (2009): Characterization of a SNPs panel for meat traceability in six cattle breeds. Food Control, 20, 856-860 https://doi.org/10.1016/j.foodcont.2008.10.015Qiu Haifang, Zhao Shuhong, Xu Xuewen, Yerle Martine, Liu Bang (2008): Assignment and expression patterns of porcine muscle-specific isoform of phosphoglycerate mutase gene. Journal of Genetics and Genomics, 35, 257-260 https://doi.org/10.1016/S1673-8527(08)60036-3Rigden Daniel J. (2008): The histidine phosphatase superfamily: structure and function. Biochemical Journal, 409, 333- https://doi.org/10.1042/BJ20071097Sauna Zuben E., Kimchi-Sarfaty Chava (): Understanding the contribution of synonymous mutations to human disease. Nature Reviews Genetics, 12, 683-691 https://doi.org/10.1038/nrg3051Stella Roberto, Biancotto Giancarlo, Krogh Morten, Angeletti Roberto, Pozza Giandomenico, Sorgato Maria Catia, James Peter, Andrighetto Igino (2011): Protein Expression Changes in Skeletal Muscle in Response to Growth Promoter Abuse in Beef Cattle. Journal of Proteome Research, 10, 2744-2757 https://doi.org/10.1021/pr101255cStuber C. W., Edwards M. D., Wendel J. F. (1987): Molecular Marker-Facilitated Investigations of Quantitative Trait Loci in Maize. II. Factors Influencing Yield and its Component Traits1. Crop Science, 27, 639- https://doi.org/10.2135/cropsci1987.0011183X002700040006xTonin Paola, Bruno Claudio, Cassandrini Denise, Savio Chiara, Tavazzi Eleonora, Tomelleri Giuliano, Piccolo Giovanni (2009): Unusual presentation of phosphoglycerate mutase deficiency due to two different mutations in PGAM-M gene. Neuromuscular Disorders, 19, 776-778 https://doi.org/10.1016/j.nmd.2009.08.007Tsujino Seiichi, Shanske Sara, Sakoda Saburo, Toscano Antonio, DiMauro Salvatore (1995): Molecular genetic studies in muscle phosphoglycerate mutase (PGAM-M) deficiency. Muscle & Nerve, 18, S50-S53 https://doi.org/10.1002/mus.880181412Watkins H. A., Baker E. N. (): Structural and Functional Analysis of Rv3214 from Mycobacterium tuberculosis, a Protein with Conflicting Functional Annotations, Leads to Its Characterization as a Phosphatase. Journal of Bacteriology, 188, 3589-3599 https://doi.org/10.1128/JB.188.10.3589-3599.2006Wimmers K., Fiedler I., Hardge T., Murani E., Schellander K., Ponsuksili S. (2006): QTL for microstructural and biophysical muscle properties and body composition in pigs. BMC Genetics, 7, 15. https://doi.org/10.1186/1471-2156-7-15Zhang Gong-Wei, Wang Hong-Ze, Chen Shi-Yi, Li Zi-Cheng, Zhang Wen-Xiu, Lai Song-Jia (2011): A reduced incidence of digestive disorders in rabbits is associated with allelic diversity at the TLR4 locus. Veterinary Immunology and Immunopathology, 144, 482-486 https://doi.org/10.1016/j.vetimm.2011.08.009Zhang Jianxuan, Yu Long, Fu Qiang, Gao Jie, Xie Yihu, Chen Jian, Zhang Pingzhao, Liu Qing, Zhao Shouyuan (2001): Mouse phosphoglycerate mutase M and B isozymes: cDNA cloning, enzyme activity assay and mapping. Gene, 264, 273-279 https://doi.org/10.1016/S0378-1119(00)00597-7