Bovine hemoglobin polymorphism: a novel alpha-globin variant identified in the Agerolese breed from southern Italy

https://doi.org/10.17221/8128-CJASCitation:Salzano A.M., Pauciullo A., D’Ambrosio C., Novi G., Strazzullo M., Scaloni A. (2015): Bovine hemoglobin polymorphism: a novel alpha-globin variant identified in the Agerolese breed from southern Italy. Czech J. Anim. Sci., 60: 145-151.
supplementary materialdownload PDF

This study describes the results of molecular screening in the Agerolese cattle breed from southern Italy, which constitutes an economical source and contributes to the regional biodiversity. Integrated electrophoretic, chromatographic, mass spectrometric, and DNA sequencing procedures were used to qualitatively and quantitatively evaluate globin chain polymorphism in a population of animals from different farms. In addition to α-, βA-, and βB-globin-bearing animal phenotypes, our data demonstrate the occurrence of individuals characterized by the presence of a novel α-globin variant, whose primary structure differs from the normal counterpart for the p.Ala27Thr substitution. The SNP c.79G>A located at the exon 1 of the HBA2 gene is responsible for this amino acid change and it represents a third example of α-globin gene polymorphism observed in Bos taurus. This phenomenon was ascertained only in two Mediterranean breeds from southern Italy and highlights the uncommon occurrence of α-globin variants in this geographical region, similarly to what has already been described in goat and human.

References:
BANGHAM A. D., BLUMBERG B. S. (1958): Distribution of Electrophoretically Different Haemoglobins among Some Cattle Breeds of Europe and Africa. Nature, 181, 1551-1552 https://doi.org/10.1038/1811551a0
 
Braend M. (1971): Haemoglobin variants in cattle. Animal Blood Groups and Biochemical Genetics, 2, 15-21 https://doi.org/10.1111/j.1365-2052.1971.tb01191.x
 
BRAEND M., EFREMOV G., RAASTAD A. (1966): GENETICS OF BOVINE HEMOGLOBIN D. Hereditas, 54, 255-259 https://doi.org/10.1111/j.1601-5223.1966.tb02020.x
 
Efremov G., Braend M. (1965): Differences in cattle globins. Biochemical Journal, 97, 867–869.
 
Haenlein G.F. W., Gonyon D.S., Mather R.E., Hines H.C. (1987): Associations of Bovine Blood and Milk Polymorphisms with Lactation Traits: Guernseys. Journal of Dairy Science, 70, 2599-2609 https://doi.org/10.3168/jds.S0022-0302(87)80329-6
 
Lacerra Giuseppina, Fiorito Mirella, Musollino Gennaro, Noce Francesca Di, Esposito Maria, Nigro Vincenzo, Gaudiano Carlo, Carestia Clementina (2004): Sequence variations of the ?-globin genes: Scanning of high CG content genes with DHPLC and DG-DGGE. Human Mutation, 24, 338-349 https://doi.org/10.1002/humu.20088
 
Montgomery G. W., Sise J. A. (1990): Extraction of DNA from sheep white blood cells. New Zealand Journal of Agricultural Research, 33, 437-441 https://doi.org/10.1080/00288233.1990.10428440
 
Namikawa Takao, Takenaka Osamu, Takahashi Kenji (1983): Hemoglobin Bali (bovine): ?A18(Bl)Lys ? his: One of the ?missing links? between ?A and ?B of domestic cattle exists in the Bali cattle (Bovinae, Bos banteng). Biochemical Genetics, 21, 787-796 https://doi.org/10.1007/BF00498925
 
Peretti Vincenzo, Ciotola Francesca, Iannuzzi Leopoldo (2013): Characterization, conservation and sustainability of endangered animal breeds in Campania (Southern Italy). Natural Science, 05, 1-9 https://doi.org/10.4236/ns.2013.55A001
 
Perutz M.F. (1990): Frequency of abnormal human haemoglobins caused by C → T transitions in CpG dinucleotides. Biophysical Chemistry, 37, 25-29 https://doi.org/10.1016/0301-4622(90)88004-C
 
Pieragostini E, Scaloni A, Rullo R, Di Luccia A (2000): Identical marker alleles in Podolic cattle (Bos taurus) and Indian zebu (Bos indicus). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 127, 1-9 https://doi.org/10.1016/S0305-0491(00)00218-2
 
Pieragostini Elisa, Rullo Rosario, Scaloni Andrea, Bramante Grazia, Di Luccia Aldo (2005): The alpha chains of goat hemoglobins: Old and new variants in native Apulian breeds. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 142, 18-27 https://doi.org/10.1016/j.cbpc.2005.05.005
 
Pieragostini Elisa, Alloggio Ingrid, Petazzi Ferruccio (2010): Insights into Hemoglobin Polymorphism and Related Functional Effects on Hematological Pattern in Mediterranean Cattle, Goat and Sheep. Diversity, 2, 679-700 https://doi.org/10.3390/d2040679
 
Pirastru Monica, Multineddu Chiara, Mereu Paolo, Sannai Mara, el Sherbini El Said, Hadjisterkotis Eleftherios, Nàhlik Andràs, Franceschi Paul, Manca Laura, Masala Bruno (2009): The sequence and phylogenesis of the α-globin genes of Barbary sheep (Ammotragus lervia), goat (Capra hircus), European mouflon (Ovis aries musimon) and Cyprus mouflon (Ovis aries ophion). Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 4, 168-173 https://doi.org/10.1016/j.cbd.2009.02.002
 
Scaloni A., Pieragostini E., Malorni A., Ferrara L., Di Luccia A. (1998): Bovine hemoglobin α-globin chain polymorphism: Primary structure determination of two new genetic variants by mass spectrometry and amino acid sequencing. Biochimie, 80, 333-338 https://doi.org/10.1016/S0300-9084(98)80076-X
 
Schroeder W.A., Shelton J.Roger, Shelton Joan Balog, Robberson Barbara, Babin Donald R. (1967): A comparison of amino acid sequences in the β-chains of adult bovine hemoglobins A and B. Archives of Biochemistry and Biophysics, 120, 124-135 https://doi.org/10.1016/0003-9861(67)90606-6
 
Storz Jay F., Moriyama Hideaki (2008): Mechanisms of Hemoglobin Adaptation to High Altitude Hypoxia. High Altitude Medicine & Biology, 9, 148-157 https://doi.org/10.1089/ham.2007.1079
 
TANAKA Kazuaki, TAKIZAWA Tatsuya, DORJI Tashi, AMANO Takashi, MANNEN Hideyuki, MAEDA Yoshizane, YAMAMOTO Yoshio, NAMIKAWA Takao (2011): Polymorphisms in the bovine hemoglobin-beta gene provide evidence for gene-flow between wild species of Bos (Bibos) and domestic cattle in Southeast Asia. Animal Science Journal, 82, 36-45 https://doi.org/10.1111/j.1740-0929.2010.00808.x
 
Weber R. E., Campbell K. L. (2011): Temperature dependence of haemoglobin-oxygen affinity in heterothermic vertebrates: mechanisms and biological significance. Acta Physiologica, 202, 549-562 https://doi.org/10.1111/j.1748-1716.2010.02204.x
 
supplementary materialdownload PDF

© 2019 Czech Academy of Agricultural Sciences