Researching rumen degradation behaviour of protein by FTIR spectroscopy D., Wu P., Jiao P. (2015): Researching rumen degradation behaviour of protein by FTIR spectroscopy. Czech J. Anim. Sci., 60: 25-32.
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The objectives of the present study were: (1) to detect protein secondary structures using the Fourier transform infrared spectroscopy (FTIR), and (2) to investigate the relationship between protein secondary structures and their rumen degradation kinetics in dairy cattle. The study was carried out using FTIR to estimate protein molecular structure of eight different types of samples, such as soybean meal, cottonseed meal, rapeseed meal, distilled dried grains with solubles, and corn. Nutritive values of these samples were determined using the Cornell Net Carbohydrate and Protein System. The results showed that: (1) different feed protein sources had different percentages and ratios of α-helixes and β-sheets in their protein secondary structures, (2) the higher protein α-helix to β-sheet ratio resulted in lower PB3 and PC, and higher PB1 and PB2 in the feeds, and (3) the α-helix to β-sheet ratio of the rumen degradation residues was changed along with the rumen degradation.
AOAC (1990): Official Methods of Analysis. 15th Ed. Association of Official Analytical Chemists, Arlington, USA.
Budevska B.O. (2004): Applications of vibrational spectroscopy in life, pharmaceutical and natural sciences. In: Chalmers J.M. and Griffiths P.R. (eds): Handbook of Vibrational Spectroscopy. John Wiley and Sons Inc., New York, USA, 3720–3732.
Chalupa W., Sniffen C.J. (1994): Carbohydrate, protein and amino acid nutrition of lactating dairy cattle. In: Garnsworty P.C. and Wiseman J. (eds): Recent Advances in Animal Nutrition. Nottingham University Press, Loughborough, UK, 265–275.
Doiron K.J., Yu P., Christensen C.R., Christensen D.A., McKinnon J.J. (2009): Detecting molecular changes in Vimy flaxseed protein structure using synchrotron FTIRM
and DFTIR spectroscopic techniques: structural and biochemical characterization. Spectroscopy, 23, 307–322.
Dyson H.Jane, Wright Peter E. (1993): Peptide conformation and protein folding. Current Opinion in Structural Biology, 3, 60-65
Goering H.K., Van Soest P.J. (1970): Forage Fiber Analysis: Apparatus, Reagents, Procedures, and Some Applications. Agricultural Handbook No. 379. Agricultural Research Service, United States Department of Agriculture, Washington, USA.
Krimm S., Bandekar J. (1986): Vibrational spectroscopy and conformation of peptides, polypeptides, and proteins. Advances in Protein Chemistry, 38, 181–364.
Krishnamoorthy U., Muscato T.V., Sniffen C.J., Van Soest P.J. (1982): Nitrogen Fractions in Selected Feedstuffs. Journal of Dairy Science, 65, 217-225
Krishnamoorthy* U., Sniffen C. J., Stern† M. D., Van Soest P. J. (1983): Evaluation of a mathematical model of rumen digestion and an in vitro simulation of rumen proteolysis to estimate the rumen-undegraded nitrogen content of feedstuffs. British Journal of Nutrition, 50, 555-568
Licitra G., Hernandez T.M., Van Soest P.J. (1996): Standardization of procedures for nitrogen fractionation of ruminant feeds. Animal Feed Science and Technology, 57, 347-358
McKinnon J.J., Olubobokun J.A., Mustafa A., Cohen R.D.H., Christensen D.A. (1995): Influence of dry heat treatment of canola meal on site and extent of nutrient disappearance in ruminants. Animal Feed Science and Technology, 56, 243-252
Miller Lisa M, Dumas Paul (2010): From structure to cellular mechanism with infrared microspectroscopy. Current Opinion in Structural Biology, 20, 649-656
Ministry of Agriculture of the P.R. China (2004): NY/T65-2004 Feeding Standard of Dairy Cow. China Agriculture Press, Beijing, P.R. China.
Orskov E.R., Hovell F.D.B., Mould F. (1980): The use of nylon bag technique for the evaluation of feedstuffs. Tropical Animal Production, 5, 195–213.
Roe M.B., Sniffen C.J., Chase L.E. (1990): Techniques for measuring protein fractions in feedstuffs. In: Proc. Cornell Nutrition Conference for Feed Manufacturers, Ithaka, USA, 81–88.
Sarver Ronald W., Krueger William C. (1991): Protein secondary structure from fourier transform infrared spectroscopy: A data base analysis. Analytical Biochemistry, 194, 89-100
Sniffen C.J., O’Connor J.D., Van Soest P.J., Fox D.G., Russell J.B. (1992): A net carbohydrate and protein system for evaluating cattle diets: II. Carbohydrate and protein system availability. Journal of Animal Science, 70, 3562–3577.
Van Soest P.J., Sniffen C.J., Mertens D.R., Fox D.G., Robinson P.H., Krishnamoorthy U.C. (1981): A net protein system for cattle: the rumen submodel for nitrogen. In: Owens F.N. (ed.): Protein Requirements for Cattle. In: Proc. Internat. Symposium (MP109-P), Stillwater, USA, 265.
Van Soest P.J., Robertson J.B., Lewis B.A. (1991): Methods for Dietary Fiber, Neutral Detergent Fiber, and Nonstarch Polysaccharides in Relation to Animal Nutrition. Journal of Dairy Science, 74, 3583-3597
Wetzel D.L., Eilert A.J., Pietrzak L.N., Miller S.S., Sweat J.A. (1998): Ultra-spatially resolved synchrotron infrared microspectroscopy of plant tissue in situ. Cellular and Molecular Biology, 44, 145–167.
Wetzel David L., Srivarin Phatthranith, Finney Jerod R. (2003): Revealing protein infrared spectral detail in a heterogeneous matrix dominated by starch. Vibrational Spectroscopy, 31, 109-114
Meng-Xia Xie, Yuan Liu (2002): Studies on the hydrogen bonding of aniline's derivatives by FT-IR. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 58, 2817-2826
Yu P. (2004): Application of advanced synchrotron radiation-based Fourier transform infrared (SR-FTIR) microspectroscopy to animal nutrition and feed science: a novel approach. British Journal of Nutrition, 92, 869-
Yu P. (2007): Protein molecular structures, protein subfractions, and protein availability affected by heat processing: a review. American Journal of Biochemistry and Biotechnology, 3, 70–90.
Yu Peiqiang, Nuez-Ortín Waldo G. (2010): Relationship of protein molecular structure to metabolisable proteins in different types of dried distillers grains with solubles: a novel approach. British Journal of Nutrition, 104, 1429-1437
Yu P., Christensen D.A., McKinnon J.J. (2003a): Comparison of the National Research Council-2001 model with the Dutch system (DVE/OEB) in the prediction of nutrient supply to dairy cows from forages. Journal of Dairy Science, 86, 2178–2192.
Yu P., McKinnon J.J., Christensen C.R., Christensen D.A. (2003b): Mapping plant composition with synchrotron infrared microspectroscopy and relation to animal nutrient utilization. In: Proc. Canadian Society of Animal Science 2003 Conference, Saskatoon, Canada, 1–20.
Yu P., McKinnon J.J., Christensen C.R., Christensen D.A., Marinkovic N.S., Miller L.M. (2003c): Chemical imaging of microstructures of plant tissues within cellular dimension using synchrotron infrared microspectroscopy. Journal of Agricultural and Food Chemistry, 51, 6062–6067.
Yu P., Christensen D.A., Christensen C.R., Drew M.D., Rossnagel B.G., McKinnon J.J (2004a): Use of synchrotron FTIR microspectroscopy to identify chemical differences in barley endosperm tissue in relation to rumen degradation characteristics. Canadian Journal of Animal Science, 84, 523–527.
Yu P., McKinnon J.J., Christensen C.R., Christensen D.A. (2004b): Using synchrotron-based FTIR microspectroscopy to reveal chemical features of feather protein secondary structure: comparison with other feed protein sources. Journal of Agricultural and Food Chemistry, 52, 7353–7361.
Yu Peiqiang, Damiran Daalkhaijav, Azarfar Arash, Niu Zhiyuan (2011): Detecting Molecular Features of Spectra Mainly Associated with Structural and Non-Structural Carbohydrates in Co-Products from BioEthanol Production Using DRIFT with Uni- and Multivariate Molecular Spectral Analyses. International Journal of Molecular Sciences, 12, 1921-1935
Zhang Xuewei, Yu Peiqiang (2012): Relationship of carbohydrate molecular spectroscopic features in combined feeds to carbohydrate utilization and availability in ruminants. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 92, 225-233
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