Oxidative stress and motility in tench Tinca tinca spermatozoa

https://doi.org/10.17221/8238-CJASCitation:Dzyuba V., Cosson J., Dzyuba B., Rodina M. (2015): Oxidative stress and motility in tench Tinca tinca spermatozoa. Czech J. Anim. Sci., 60: 250-262.
download PDF
The attachment of the urinary bladder to the seminal duct near the anal aperture in tench constitutes a potential risk for urine contamination of sperm during collection, leading to spontaneous activation of sperm motility by urine hypotonicity. It was hypothesized that sperm hypotonic exposure can provoke oxidative stress which could be involved in sperm quality degradation. Our study aimed to describe spermatozoa motility parameters and levels of oxidative stress in activating media (AM) of differing osmolality. Tench sperm samples were collected from 6 males into Kurokura 180 immobilizing medium (IM) (180mM NaCl, 2.68mM KCl, 1.36mM CaCl2 2H2O, 2.38mM NaHCO3, 340 mOsm/kg). Motility was recorded in AM of 0 mOsm/kg or 100 mOsm/kg using video microscopy combined with stroboscopic illumination. Video records were analyzed to calculate spermatozoa curvilinear velocity (VCL), motility rate, and motility duration. The level of thiobarbituric acid reactive substances (TBARS), measured by spectrophotometry, was used as an oxidative stress index. VCL and motility rate during the initial phase of motility (10 s post-activation) were not dependent on AM osmolality, while motility duration was significantly increased with 100 mOsm/kg AM. TBARS was significantly increased with reduction of AM osmolality. Increased TBARS was observed even at 5 s post-activation with AM of 0 mOsm/kg. These observations suggest that even a short period of sperm exposure to hypotonic conditions induces oxidative stress. Any contact of sperm with hypotonic urine during sperm collection should be avoided. The use of motility AM of moderate hypotonicity (≥ 100 mOsm/kg) is recommended for tench propagation.
Alavi S.M. Hadi, Rodina Marek, Policar Tomáš, Linhart Otomar (2009): Relationship between semen characteristics and body size in Barbus barbus L. (Teleostei: Cyprinidae) and effects of ions and osmolality on sperm motility. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 153, 430-437  https://doi.org/10.1016/j.cbpa.2009.04.001
asakawa T., Matsushita S. (1980): Coloring conditions of thiobarbituric acid test for detecting lipid hydroperoxides. Lipids, 15, 137-140  https://doi.org/10.1007/BF02540959
Boryshpolets Sergey, Dzyuba Boris, Drokin Sergey (2009): Pre-spawning water temperature affects sperm respiration and reactivation parameters in male carps. Fish Physiology and Biochemistry, 35, 661-668  https://doi.org/10.1007/s10695-008-9292-4
Celada Jesús D., Aguilera Amelia, García Vanesa, Carral José M., Sáez-Royuela María, González Rocío, González Álvaro (2009): Rearing juvenile tench (Tinca tinca L.) under controlled conditions using Artemia nauplii as supplement to a dry diet. Aquaculture International, 17, 565-570  https://doi.org/10.1007/s10499-008-9225-3
de Lamirande E (): Capacitation as a regulatory event that primes spermatozoa for the acrosome reaction and fertilization. Molecular Human Reproduction, 3, 175-194  https://doi.org/10.1093/molehr/3.3.175
Dobretsov G.E., Borschevskaya T.A., Petrov V.A., Vladimirov Yu.A. (1977): The increase of phospholipid bilayer rigidity after lipid peroxidation. FEBS Letters, 84, 125-128  https://doi.org/10.1016/0014-5793(77)81071-5
Drokin Sergey I. (1993): Phospholipid distribution and fatty acid composition of phosphatidylcholine and phosphatidylethanolamine in sperm of some freshwater and marine species of fish. Aquatic Living Resources, 6, 49-56  https://doi.org/10.1051/alr:1993005
Dzyuba Viktoriya, Cosson Jacky (2014): Motility of fish spermatozoa: from external signaling to flagella response. Reproductive Biology, 14, 165-175  https://doi.org/10.1016/j.repbio.2013.12.005
GELA DAVID, FLAJŠHANS MARTIN, KOCOUR MARTIN, RODINA MAREK, LINHART OTOMAR (2006): Tench (Tinca tinca) broodstock management in breeding station under conditions of pond culture: a review. Aquaculture International, 14, 195-203  https://doi.org/10.1007/s10499-005-9025-y
Krasznai Z., Marian T., Izumi H., Damjanovich S., Balkay L., Tron L., Morisawa M. (2000): Membrane hyperpolarization removes inactivation of Ca2+ channels, leading to Ca2+ influx and subsequent initiation of sperm motility in the common carp. Proceedings of the National Academy of Sciences, 97, 2052-2057  https://doi.org/10.1073/pnas.040558097
Krasznai Z (2003): Role of ion channels and membrane potential in the initiation of carp sperm motility. Aquatic Living Resources, 16, 445-449  https://doi.org/10.1016/S0990-7440(03)00054-8
Lenzi A (1996): Lipids of the sperm plasma membrane: from polyunsaturated fatty acids considered as markers of sperm function to possible scavenger therapy. Human Reproduction Update, 2, 246-256  https://doi.org/10.1093/humupd/2.3.246
Linhart O., Rodina M., Bastl J., Cosson J. (2003): Urinary bladder, ionic composition of seminal fluid and urine with characterization of sperm motility in tench (Tinca tinca L.). Journal of Applied Ichthyology, 19, 177-181  https://doi.org/10.1046/j.1439-0426.2003.00470.x
Linhart O., Alavi S. M. H., Rodina M., Gela D., Cosson J. (2008): Comparison of sperm velocity, motility and fertilizing ability between firstly and secondly activated spermatozoa of common carp ( Cyprinus carpio ). Journal of Applied Ichthyology, 24, 386-392  https://doi.org/10.1111/j.1439-0426.2008.01138.x
Mamcarz Andrzej, Kucharczyk Dariusz, Kujawa Roman (2006): Reciprocal hybrids of tench Tinca tinca (L.) × bream Abramis brama (L.), and tench × carp Cyprinus carpio L., and some characteristics of their early development. Aquaculture International, 14, 27-33  https://doi.org/10.1007/s10499-005-9011-4
Morisawa M., Suzuki K., Shimizu H., Morisawa S., Yasuda K. (1983): Effects of osmolality and potassium on motility of spermatozoa from freshwater cyprinid fishes. The Journal of Experimental Biology, 107, 95–103.
Nolan J.P., Hammerstedt R.H. (1997): Regulation of membrane stability and the acrosome reaction in mammalian sperm. The FASEB Journal, 11, 670–682.
Perchec G., Jeulin C., Cosson J., Andre F., Billard R. (1995): Relationship between sperm ATP content and motility of carp spermatozoa. Journal of Cell Science, 108, 747–753.
Poupard G. P., Gatti J. L., Cosson J., Jeulin C., Fierville F., Billard R. (1997): Effects of extracellular environment on the osmotic signal transduction involved in activation of motility of carp spermatozoa. Reproduction, 110, 315-327  https://doi.org/10.1530/jrf.0.1100315
Petcoff D. W., Holland W. L., Stith B. J. (2008): Lipid levels in sperm, eggs, and during fertilization in Xenopus laevis. The Journal of Lipid Research, 49, 2365-2378  https://doi.org/10.1194/jlr.M800159-JLR200
Podhorec P. (2011): Artificial reproduction of tench (Tinca tinca L.), with an emphasis placed on hormonal induction of ovulation. PhD Diss. Vodňany, Czech Republic: University of South Bohemia in České Budějovice, 75 p.
Rodina M., Cosson J., Gela D., Linhart O. (2004): Kurokura Solution as Immobilizing Medium for Spermatozoa of Tench (Tinca tinca L.). Aquaculture International, 12, 119-131  https://doi.org/10.1023/B:AQUI.0000017192.75993.e3
Rodina M., Gela D., Kocour M., Alavi S.M. Hadi, Hulak M., Linhart O. (2007): Cryopreservation of tench, Tinca tinca, sperm: Sperm motility and hatching success of embryos. Theriogenology, 67, 931-940  https://doi.org/10.1016/j.theriogenology.2006.11.007
Surai P. F., Fujihara N., Speake B. K., Brillard J-P., Wishart G. J., Sparks N. H. C. (2001): Polyunsaturated Fatty Acids, Lipid Peroxidation and Antioxidant Protection in Avian Semen - Review -. Asian-Australasian Journal of Animal Sciences, 14, 1024-1050  https://doi.org/10.5713/ajas.2001.1024
Targońska Katarzyna, Perkowski Tomasz, Żarski Daniel, Krejszeff Sławomir, Mamcarz Andrzej, Kujawa Roman, Kucharczyk Dariusz (2012): Method of evaluation of wild common tench, Tinca tinca (L.), female suitability for artificial reproduction during the spawning season. Italian Journal of Animal Science, 11, -  https://doi.org/10.4081/ijas.2012.e30
Wang Jiaxi, Min Wenqiang, Guan Min, Gong Luojun, Ren Jie, Huang Zhen, Zheng Hongping, Zhang Jinping, Liu Huiji, Han Yuzhang (2006): Tench farming in China: present status and future prospects. Aquaculture International, 14, 205-208  https://doi.org/10.1007/s10499-005-9026-x
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti