Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls X.-., Hong J.-., Yan G.-., Wang Y.-., Li Q.-., Hu J.-. (2015): Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls. Czech J. Anim. Sci., 60: 256-262.
download PDF

Heat shock protein 70 (HSP70) is considered as a gene which affects semen quality traits. The present study attempted to investigate the relationship between the HSP70 expression level and motility of bull sperm during the process of freezing-thawing. Semen samples were collected from 5 QinChuan bulls by artificial vagina. Sperm motility and plasma membrane integrity of the semen samples at three stages (fresh, after equilibration, and frozen-thawed) were evaluated. The HSP70 expression level at the three stages was detected using real-time PCR. The results indicated that HSP70 expression level, membrane integrity, and sperm motility in the fresh semen were higher than those of the sperm after equilibration and freezing-thawing (P < 0.05), the HSP70 expression level, plasma membrane integrity, and sperm motility in sperm after equilibration were higher than those of the frozen-thawed sperm (P < 0.05). The correlation between HSP70 expression level and sperm motility was positive (ranging from 0.327 to 0.785). The results suggest that HSP70 expression level in bull spermatozoa was gradually decreased following the process of freezing-thawing, and might be associated with bull sperm motility.

Aboagla E. M.-E. (2003): Trehalose-Enhanced Fluidity of the Goat Sperm Membrane and Its Protection During Freezing. Biology of Reproduction, 69, 1245-1250
Arispe Nelson, Doh Michael, De Maio Antonio (2002): Lipid interaction differentiates the constitutive and stress-induced heat shock proteins Hsc70 and Hsp70. Cell Stress & Chaperones, 7, 330-<0330:LIDTCA>2.0.CO;2
Beere H (): Stress management – heat shock protein-70 and the regulation of apoptosis. Trends in Cell Biology, 11, 6-10
Borkovich K.A., Farrelly F.W., Finkelstein D.B., Taulien J., Lindquist S. (1989): Hsp82 is an essential protein that is required in higher concentrations for growth of cells at higher temperatures. Molecular and Cellular Biology, 9, 3919–3930.
Cao W.L., Wang Y.X., Xiang Z.Q., Li Z. (2003): Cryopreservation-induced decrease in heat-shock protein 90 in human spermatozoa and its mechanism. Asian Journal of Andrology, 5, 43–46.
Casas I., Sancho S., Ballester J., Briz M., Pinart E., Bussalleu E., Yeste M., Fàbrega A., Rodríguez-Gil J.E., Bonet S. (2010): The HSP90AA1 sperm content and the prediction of the boar ejaculate freezability. Theriogenology, 74, 940-950
Chen G.S., Xu S.F. (1998): Heat shock protein and apoptosis. Medical Oncology Fascicule Abroad, 23, 332–334.
Eduardo R.P., Diez C., Lapena A.C., Perez-Martos A., Montoya J., Alvarez E., Arenas J., Lopez-Perez M.J. (1998): Correlation of sperm motility with mitochondrial enzymatic activities. Clinical Chemistry, 44, 1616–1620.
Gabai V. L., Meriin A. B., Mosser D. D., Caron A. W., Rits S., Shifrin V. I., Sherman M. Y. (): Hsp70 Prevents Activation of Stress Kinases: A NOVEL PATHWAY OF CELLULAR THERMOTOLERANCE. Journal of Biological Chemistry, 272, 18033-18037
Gething Mary-Jane, Sambrook Joseph (1992): Protein folding in the cell. Nature, 355, 33-45
Guo X.Z., Xu R.B. (1997): The mechanism of protein synthesis inhibition in heat shock response. Chemistry of Life, 17, 22–24.
Gutiérrez-Pérez Oscar, Juárez-Mosqueda María de Lourdes, Carvajal Salvador Uribe, Ortega María Elena Trujillo (2009): Boar spermatozoa cryopreservation in low glycerol/trehalose enriched freezing media improves cellular integrity. Cryobiology, 58, 287-292
Harding Heather P, Novoa Isabel, Zhang Yuhong, Zeng Huiqing, Wek Ron, Schapira Matthieu, Ron David (2000): Regulated Translation Initiation Controls Stress-Induced Gene Expression in Mammalian Cells. Molecular Cell, 6, 1099-1108
Hu J.-H., Zhao X.-L., Tian W.-Q., Zan L.-S., Li Q.-W. (2011): Effects of vitamin E supplementation in the extender on frozen-thawed bovine semen preservation. animal, 5, 107-112
Huang S.Y, Kuo Y.H, Lee W.C, Tsou H.L, Lee Y.P, Chang H.L, Wu J.J, Yang P.C (1999): Substantial decrease of heat-shock protein 90 precedes the decline of sperm motility during cooling of boar spermatozoa. Theriogenology, 51, 1007-1016
Huang S.Y., Kuo Y.H., Tsou H.L., Lee Y.P., King Y.T., Huang H.C., Yang P.C., Lee W.C. (2000): The decline of porcine sperm motility by geldanamycin, a specific inhibitor of heat-shock protein 90 (hsp90). Theriogenology, 53, 1177-1184
Kamaruddin M., Kroetsch T., Basrur P.K., King W.A. (1996): Heat shock protein 70 in bovine semen. Biology of Reproduction, 54, 222.
Lewis S.E.M., Donnelly E.T., Sterling E.S.L., Kennedy M.S., Thompson W., Chakravarthy U. (1996): Regulators of sperm function. Molecular Human Reproduction, 2, 873-878
Li G.C. (1987): Heat shock proteins: role in thermotolerance, drug resistance, and relationship to DNA topoisomerases. NCI Monographs, 4, 99–103.
Lima S., Cedenho A., Cenedeze M., Bertolla R., Oehninger S., Srougi M. (2004): Male infertility and heat shock protein 70–2 (HSP70–2) expression. Fertility and Sterility, 82, S181-
Martin G., Cagnon N., Sabido O., Sion B., Grizard G., Durand P., Levy R. (2006): Kinetics of occurrence of some features of apoptosis during the cryopreservation process of bovine spermatozoa. Human Reproduction, 22, 380-388
Mayer M. P., Bukau B. (2005): Hsp70 chaperones: Cellular functions and molecular mechanism. Cellular and Molecular Life Sciences, 62, 670-684
MESTRIL R, DILLMANN W (1995): Heat shock proteins and protection against myocardial ischemia. Journal of Molecular and Cellular Cardiology, 27, 45-52
Osinowo O. A., Bale J. O., Oyedipe E. O., Eduvie L. O. (1982): Motility and eosin uptake of formaldehyde-treated ram spermatozoa. Reproduction, 65, 389-394
Pearl Laurence H, Prodromou Chrisostomos (2000): Structure and in vivo function of Hsp90. Current Opinion in Structural Biology, 10, 46-51
Roca J. (2006): Factors influencing boar sperm cryosurvival. Journal of Animal Science, 84, 2692-2699
Ruiz-Pesini E., Diez C., Lapena A.C., Perez-Martos A., Montoya J., Alvarez E., Arenas J., Lopez-Perez M.J. (1998): Correlation of sperm motility with mitochondrial enzymatic activities. Clinical Chemistry, 44, 1616–1620.
Vijayaraghavan S. (1996): Sperm motility development in the epididymis is associated with decreased glycogen synthase kinase-3 and protein phosphatase 1 activity. Biology of Reproduction, 54, 709-718
Wang Peng, Wang Yan-Feng, Wang Hong, Wang Chun-Wei, Zan Lin-Sen, Hu Jian-Hong, Li Qing-Wang, Jia Yong-Hong, Ma Guo-Ji (2014): HSP90 expression correlation with the freezing resistance of bull sperm. Zygote, 22, 239-245
Christian Wigley W. (): Dynamic Association of Proteasomal Machinery with the Centrosome. The Journal of Cell Biology, 145, 481-490
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti