Allele frequency for c.335 A>C polymorphisms in porcine ghrelin/obestatin prepropeptide gene and association analysis with performance traits in various pig breeds
K. Tanaka, T. Takizawa, O. Oki, K. Fukawa, T. Ito, M. Miyabe, H. Mannen, Y. Kurosawa, K. Hirosehttps://doi.org/10.17221/8459-CJASCitation:Tanaka K., Takizawa T., Oki O., Fukawa K., Ito T., Miyabe M., Mannen H., Kurosawa Y., Hirose K. (2015): Allele frequency for c.335 A>C polymorphisms in porcine ghrelin/obestatin prepropeptide gene and association analysis with performance traits in various pig breeds. Czech J. Anim. Sci., 60: 411-416.
The allelic frequency of c.335A>C polymorphisms in the porcine ghrelin/obestatin prepropeptide (GHRL) gene was surveyed among six pig breeds and two subspecies of wild boars. The c.335C was the most frequent allele in Berkshire, Landrace, Large White, Yorkshire, and Clawn miniature pigs and Ryukyu wild boars (Sus scrofa riukiuanus), whereas c.335A was the most frequent allele in Duroc and Meishan pigs and Japanese wild boars (S. s. leucomystax). The association of c.335A>C with performance traits was analyzed in Duroc, Landrace, Large White, and (Landrace × Large White) × Duroc (LWD) cross-bred pigs. No associations between c.335A>C genotype and average daily weight gain, backfat thickness, or intramuscular fat were detected. However, an association was observed between loin eye muscle area (EMA) and c.335A>C genotype in Duroc gilts. The AA genotype group had larger EMA than the AC genotype group in Duroc gilts; however, this association was not significant in Duroc boars and barrows or the other pig populations investigated. These results demonstrate that GHRL c.335A>C is not a major quantitative trait loci candidate on pig chromosome 13 affecting fat deposition.Keywords:quantitative trait locus; GHRL; pigs; polymorphism; genetic markerReferences:
Chartrel N., Alvear-Perez R., Leprince J., Iturrioz X., Goazigo A. R.-L., Audinot V., Chomarat P., Coge F., Nosjean O., Rodriguez M., Galizzi J. P., Boutin J. A., Vaudry H., Llorens-Cortes C. (2007): Comment on "Obestatin, a Peptide Encoded by the Ghrelin Gene, Opposes Ghrelin's Effects on Food Intake". Science, 315, 766c-766c https://doi.org/10.1126/science.1135047Fontanesi L., Galimberti G., Calo D. G., Fronza R., Martelli P. L., Scotti E., Colombo M., Schiavo G., Casadio R., Buttazzoni L., Russo V. (): Identification and association analysis of several hundred single nucleotide polymorphisms within candidate genes for back fat thickness in Italian Large White pigs using a selective genotyping approach. Journal of Animal Science, 90, 2450-2464 https://doi.org/10.2527/jas.2011-4797Gourcerol G., St-Pierre D.H., Tache Y. (2007): Lack of obestatin effects on food intake: should obestatin be renamed ghrelin-associated peptide (GAP)? Regulatory Peptides, 141, 1–7.Jones G.F. (1998): Genetic aspects of domestication, common breeds and their origin. In: Ruvinsky A. and Rothschild M.F. (eds): The Genetics of the Pig. CAB International, Oxon, UK, 17–50.Kim Kwan-Suk, Thomsen Hauke, Bastiaansen John, Nguyen Nguyet Thu, Dekkers Jack C.M., Plastow Graham S., Rothschild Max F. (2004): Investigation of Obesity Candidate Genes On Porcine Fat Deposition Quantitative Trait Loci Regions. Obesity Research, 12, 1981-1994 https://doi.org/10.1038/oby.2004.249Kojima M., Hosoda H., Date Y., Nakazato M., Matsuo H., Kangawa K. (1999): Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature, 402, 656–660. https://doi.org/10.1038/45230Korbonits Márta, Gueorguiev Maria, O’Grady Eithne, Lecoeur Cecile, Swan Daniel C., Mein Charles A., Weill Jacques, Grossman Ashley B., Froguel Philippe (2002): A Variation in the Ghrelin Gene Increases Weight and Decreases Insulin Secretion in Tall, Obese Children. The Journal of Clinical Endocrinology & Metabolism, 87, 4005-4008 https://doi.org/10.1210/jcem.87.8.8881Matsumoto Masaru, Kitajima Yasuo, Iwanami Tatsuya, Hayashi Yujiro, Tanaka Shoji, Minamitake Yoshiharu, Hosoda Hiroshi, Kojima Masayasu, Matsuo Hisayuki, Kangawa Kenji (2001): Structural Similarity of Ghrelin Derivatives to Peptidyl Growth Hormone Secretagogues. Biochemical and Biophysical Research Communications, 284, 655-659 https://doi.org/10.1006/bbrc.2001.5014Giudice E Miraglia del, Santoro N, Cirillo G, Raimondo P, Grandone A, D'Aniello A, Nardo M Di, Perrone L (): Molecular screening of the ghrelin gene in Italian obese children: the Leu72Met variant is associated with an earlier onset of obesity. International Journal of Obesity, 28, 447-450 https://doi.org/10.1038/sj.ijo.0802572Oishi T., Nakanishi Y., Tanaka K. (1991): Genetic analysis of Clawn miniature pigs by blood groups and biochemical polymorphisms. Japanese Journal of Swine Science, 28, 126–132. (in Japanese)Peino R, Baldelli R, Rodriguez-Garcia J, Rodriguez-Segade S, Kojima M, Kangawa K, Arvat E, Ghigo E, Dieguez C, Casanueva F. (2000): Ghrelin-induced growth hormone secretion in humans. European Journal of Endocrinology, 143, R11-R14 https://doi.org/10.1530/eje.0.143R011Sambrook J., Fritsch E.F., Maniatis T. (1989): Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York, USA.Sanchez Marie-Pierre, Iannuccelli Nathalie, Basso Benjamin, Bidanel Jean-Pierre, Billon Yvon, Gandemer Gilles, Gilbert Hélène, Larzul Catherine, Legault Christian, Riquet Juliette, Milan Denis, Le Roy Pascale (): Identification of QTL with effects on intramuscular fat content and fatty acid composition in a Duroc × Large White cross. BMC Genetics, 8, 55- https://doi.org/10.1186/1471-2156-8-55Sato S., Oyamada Y., Atsuji K., Nade T., Sato S., Kobayashi E., Mitsuhashi T., Nirasawa K., Komatsuda A., Saito Y., Terai S., Hayashi T., Sugimoto Y. (2003): Quantitative trait loci analysis for growth and carcass traits in a Meishan × Duroc F2 resource population. Journal of Animal Science, 81, 2938–2949.Tang Sheng-Qiu, Jiang Qing-Yan, Zhang Yong-Liang, Zhu Xiao-Tong, Shu Gang, Gao Ping, Feng Ding-Yuan, Wang Xiu-Qi, Dong Xiao-Ying (2008): Obestatin: Its physicochemical characteristics and physiological functions. Peptides, 29, 639-645 https://doi.org/10.1016/j.peptides.2008.01.012Tomás A., Ramírez O., Casellas J., Muñoz G., Sánchez A., Barragán C., Arqué M., Riart I., Óvilo C., Noguera J. L., Amills M., Rodríguez C. (2011): Quantitative trait loci for fatness at growing and reproductive stages in Iberian × Meishan F2 sows. Animal Genetics, 42, 548-551 https://doi.org/10.1111/j.1365-2052.2010.02169.xWatanobe T., Okumura N., Ishiguro N., Nakano M., Matsui A., Sahara M., Komatsu M. (1999): Genetic relationship and distribution of the Japanese wild boar (Sus scrofa leucomystax) and Ryukyu wild boar (Sus scrofa riukiuanus) analysed by mitochondrial DNA. Molecular Ecology, 8, 1509-1512 https://doi.org/10.1046/j.1365-294x.1999.00729.xWojtysiak Dorota, Kaczor Urszula (2011): Effect of polymorphisms at the ghrelin gene locus on carcass, microstructure and physicochemical properties of longissimus lumborum muscle of Polish Landrace pigs. Meat Science, 89, 514-518 https://doi.org/10.1016/j.meatsci.2011.05.019Zhang J. V. (2005): Obestatin, a Peptide Encoded by the Ghrelin Gene, Opposes Ghrelin's Effects on Food Intake. Science, 310, 996-999 https://doi.org/10.1126/science.1117255Zhang J. V., Klein C., Ren P.-G., Kass S., Donck L. V., Moechars D., Hsueh A. J. W. (2007): Response to Comment on "Obestatin, a Peptide Encoded by the Ghrelin Gene, Opposes Ghrelin's Effects on Food Intake". Science, 315, 766d-766d https://doi.org/10.1126/science.1137136