Characterization of greying, melanoma, and vitiligo quantitative inheritance in Old Kladruber horses

https://doi.org/10.17221/8524-CJASCitation:Hofmanová B., Vostrý L., Majzlík I., Vostrá-Vydrová H. (2015): Characterization of greying, melanoma, and vitiligo quantitative inheritance in Old Kladruber horses. Czech J. Anim. Sci., 60: 443-451.
download PDF
The paper deals with the greying process, melanoma, and vitiligo depigmentation occurrence in Old Kladruber horses (OKH). The study includes 376 animals of both sexes at the age of 1–25 years. The evaluation was performed repeatedly during 4 consecutive years. The greying status was measured spectrophotometrically on four body parts using L*a*b* colour system. Melanoma and vitiligo were detected visually and by palpation in all the animals and classified using 5 (3) grade scale. The GLM procedure of SAS package was used to examine the influence of the effects of line, age, sex, stud, and year of evaluation. The GLM analysis confirmed a significant impact of age on greying. Parameter L* showed progressive increase until 10 years of age when all horses reached final grey level. Subsequent analyses suggested the influence of sex, i.e. a notably higher level of greying in mares. The incidence of melanoma was confirmed. Melanoma in OKH most frequently occurs on the bottom of the tail or at the anal and perineal area. Probably only benign forms of melanoma have been detected. The global occurrence of melanoma in OKH (ca. 13%) is substantially lower than in Lipizzan and Camargue horses. Overall incidence of melanoma in horses at the age of 15 and older reached 68%. Genetic parameters were estimated for melanoma grade, grey level, and vitiligo. Greying is strictly influenced by age (h2 = 0.52 ± 0.07). Prevalence of melanoma progresses with age (h2 = 0.07 ± 0.04). Vitiligos of both facial and anal parts are influenced by the effects studied (h2 = 0.20 ± 0.05 and 0.34 ± 0.06 for vitiligo A and vitiligo F, respectively).
References:
Bertone J.J. (2006): Equine Geriatric Medicine and Surgery. Elsevier, St. Louis, USA.
 
Curik I., Seltenhammer M., Solkner J. (2002): Quantitative genetic analysis of melanoma and grey level in Lipizzan horses. In: Proc. 7th World Congress on Genetics Applied to Livestock Production, Montpellier, France, 19–23.
 
Curik I., Seltenhammer M., Toth S., Niebauer G., Solkner J. (2004): Quantitative inheritance of the coat greying process in horse. In: Proc. 52th Annu. Mtg., European Association for Animal Production (EAAP), Bled, Slovenia, 316.
 
Curik Ino, Druml Thomas, Seltenhammer Monika, Sundström Elisabeth, Pielberg Gerli Rosengren, Andersson Leif, Sölkner Johann, Goddard Mike E. (2013): Complex Inheritance of Melanoma and Pigmentation of Coat and Skin in Grey Horses. PLoS Genetics, 9, e1003248-  https://doi.org/10.1371/journal.pgen.1003248
 
Falconer D.S., Mackay T.F.C. (1996): Introduction into Quantitative Genetics. Longman, Harlow, UK.
 
Fleury C., Berard F., Balme B., Thomas L. (2000a): The study of cutaneous melanomas in Camarque-type gray skinned horses (1): clinical-pathological characterization. Pigment Cell Research, 13, 39–46.
 
Fleury C., Berard F., Leblond A., Faure C., Ganem N., Thomas L. (2000b): The study of cutaneous melanomas in Camargue-type gray skinned horses (2): Epidemiological survey. Pigment Cell Research, 13, 47–51.
 
Futas J., Vychodilova L., Hofmanova B., Vranova M., Putnova L., Muzik J., Vyskocil M., Vrtkova I., Dusek L., Majzlik I., Horin P. (2012): Genomic analysis of resistance/susceptibility to melanoma in Old Kladruber greying horses. Tissue Antigens, 79, 247-248  https://doi.org/10.1111/j.1399-0039.2011.01827.x
 
Heinzerling Lucie M., Feige Karsten, Rieder Stefan, Akens Margarete K., Dummer Reinhard, Stranzinger Gerald, Moelling Karin (2001): Tumor regression induced by intratumoral injection of DNA coding for human interleukin 12 into melanoma metastases in gray horses. Journal of Molecular Medicine, 78, 692-702  https://doi.org/10.1007/s001090000165
 
Henner J.E. (2002): Molecular genetic studies on the coat colour inheritance in horses with special emphasis on Freiberger breed. PhD Diss. Zürich Univ. 119 p. Available from: http://aleph-www.ub.fu-berlin.de. (in German)
 
Henner Julia, Poncet Pierre-André, Guérin Gérard, Hagger Christian, Stranzinger Gerald, Rieder Stefan (2002): Genetic mapping of the (G)-locus, responsible for the coat color phenotype "progressive greying with age" in horses (Equus caballus). Mammalian Genome, 13, 535-537  https://doi.org/10.1007/s00335-002-2174-7
 
Horín P., Cothran E. G., Trtková K., Marti E., Glasnák V., Henney P., Vyskocil M., Lazary S. (1998): Polymorphism of Old Kladruber horses, a surviving but endangered baroque breed. International Journal of Immunogenetics, 25, 357-363  https://doi.org/10.1111/j.1744-313X.1998.tb01153.x
 
Jakubec V., Vostry L., Schlote W., Majzlik I., Mach K. (2009): Selection in the genetic resource: genetic variation of the linear described type traits in the Old Kladrub horse. Archiv Tierzucht, 52, 343–355.
 
Janova Eva, Futas Jan, Klumplerova Marie, Putnova Lenka, Vrtkova Irena, Vyskocil Mirko, Frolkova Petra, Horin Petr (2013): Genetic diversity and conservation in a small endangered horse population. Journal of Applied Genetics, 54, 285-292  https://doi.org/10.1007/s13353-013-0151-3
 
Jeglum K.A. (1999): Melanomas. In: Robinson N.E. (ed.): Current Therapy in Equine Medicine. W.B. Saunders Company, Philadelphia, USA, 399–400.
 
Jorjani H., Klei L., Emanuelson U. (2003): A Simple Method for Weighted Bending of Genetic (Co)variance Matrices. Journal of Dairy Science, 86, 677-679  https://doi.org/10.3168/jds.S0022-0302(03)73646-7
 
Kovac M., Groeneveld E., Garcia-Cortes L.A. (2002): VCE 5, a package for the estimation of dispersion parameters. In: Proc. 7th World Congress on Genetics Applied to Livestock Production. INRA, Castanet-Tolosan, France, 33, 741–742.
 
Locke M. M., Penedo M. C. T., Bricker S. J., Millon L. V., Murray J. D. (2002): Linkage of the grey coat colour locus to microsatellites on horse chromosome 25. Animal Genetics, 33, 329-337  https://doi.org/10.1046/j.1365-2052.2002.00885.x
 
Rosengren Pielberg Gerli, Golovko Anna, Sundström Elisabeth, Curik Ino, Lennartsson Johan, Seltenhammer Monika H, Druml Thomas, Binns Matthew, Fitzsimmons Carolyn, Lindgren Gabriella, Sandberg Kaj, Baumung Roswitha, Vetterlein Monika, Strömberg Sara, Grabherr Manfred, Wade Claire, Lindblad-Toh Kerstin, Pontén Fredrik, Heldin Carl-Henrik, Sölkner Johann, Andersson Leif (): A cis-acting regulatory mutation causes premature hair graying and susceptibility to melanoma in the horse. Nature Genetics, 40, 1004-1009  https://doi.org/10.1038/ng.185
 
Rieder S. (1999): Applied comparative genetics of melanoma using the horse as an example. PhD Diss. Zürich Univ. 132 p. Available from: https://e-collection.library.ethz.ch/view/eth:22891. (in German)
 
Rieder S., Stricker C., Joerg H., Dummer R., Stranzinger G. (2000): A comparative genetic approach for the investigation of ageing grey horse melanoma. Journal of Animal Breeding and Genetics, 117, 73-82  https://doi.org/10.1111/j.1439-0388.2000x.00245.x
 
Rodríguez M., García-Barona V., Peña L., Castaño M., Rodríguez A. (1997): Grey horse melanotic condition: A pigmentary disorder. Journal of Equine Veterinary Science, 17, 677-681  https://doi.org/10.1016/S0737-0806(97)80270-5
 
SELTENHAMMER M. H., SIMHOFER H., SCHERZER S., ZECHNER P., CURIK I., SÖLKNER J., BRANDT S. M., JANSEN B., PEHAMBERGER H., EISENMENGER E. (2003): Equine melanoma in a population of 296 grey Lipizzaner horses. Equine Veterinary Journal, 35, 153-157  https://doi.org/10.2746/042516403776114234
 
Seltenhammer Monika Heidemarie, Heere-Ress Elisabeth, Brandt Sabine, Druml Thomas, Jansen Burkhard, Pehamberger Hubert, Niebauer Gert Willhelm (2004): Comparative Histopathology of Grey-Horse-Melanoma and Human Malignant Melanoma. Pigment Cell Research, 17, 674-681  https://doi.org/10.1111/j.1600-0749.2004.00192.x
 
Smith S. H., Goldschmidt M. H., McManus P. M. (2002): A Comparative Review of Melanocytic Neoplasms. Veterinary Pathology, 39, 651-678  https://doi.org/10.1354/vp.39-6-651
 
Solkner J., Seltenhammer M., Curik I., Niebauer G. (2004): Genetic relationship between speed of greying, melanoma and vitiligo prevalence in grey horses. In: Proc. 52th Annu. Mtg., European Association for Animal Production (EAAP), Bled, Slovenia, 83.
 
Sponenberg P. (1996): Equine Color Genetics. Iowa State University Press, Ames, USA.
 
Swinburne J. E., Hopkins A., Binns M. M. (2002): Assignment of the horse grey coat colour gene to ECA25 using whole genome scanning. Animal Genetics, 33, 338-342  https://doi.org/10.1046/j.1365-2052.2002.00895.x
 
Toth Z. (2006): Quantitative genetic aspects of coat color in horses. Journal of Animal Science, 84, 2623-2628  https://doi.org/10.2527/jas.2005-704
 
Valentine Beth A. (1995): Equine Melanocytic Tumors: A Retrospective Study of 53 Horses (1988 to 1991). Journal of Veterinary Internal Medicine, 9, 291-297  https://doi.org/10.1111/j.1939-1676.1995.tb01087.x
 
Vostry L., Kracikova O., Hofmanova B., Czernekova V., Kott T., Pribyl J. (2011): Intra-line and inter-line genetic diversity in sire lines of the Old Kladruber horse based on microsatellite analysis of DNA. Czech Journal of Animal Science, 56, 163–175.
 
Vostry L., Hofmanova B., Vostra Vydrova H., Pribyl J., Majzlik I. (2012): Estimation of genetic parameters for melanoma in the Old Kladruber horse. Czech Journal of Animal Science, 57, 75–82.
 
download PDF

© 2019 Czech Academy of Agricultural Sciences