Tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in prolific and non-prolific goat breeds

https://doi.org/10.17221/8525-CJASCitation:Pan Z.Y., Di R., Tang Q.Q., Jin H.H., Chu M.X., Huang D.W., He J.N., Liu Q.Y., Hu W.P., Wang X.Y., Yao Y.X., Liu L., Song C.L. (2015): Tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in prolific and non-prolific goat breeds. Czech J. Anim. Sci., 60: 452-458.
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The tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in goats were investigated. The mRNA expression of the genes was detected in prolific Jining Grey goats and non-prolific Liaoning Cashmere goats by reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR. The results showed that GDF9 and BMPR1B were widely expressed in 20 tissues, while BMP15 gene was expressed exclusively in ovary and pituitary. The expression levels of GDF9 and BMPR1B genes were the highest in ovary. The expression level of BMP15 gene in the ovary of Jining Grey goat was 4.96-fold higher than that in Liaoning Cashmere goat (P < 0.05), but there were no differences (P > 0.05) in the expression level of GDF9 and BMPR1B genes. This is the first study to analyze the tissue expression pattern of GDF9, BMP15, and BMPR1B genes in goat, and the BMP15 might be a major gene for the prolificacy of Jining Grey goat.
Al-Musawi Sara L., Walton Kelly L., Heath Derek, Simpson Courtney M., Harrison Craig A. (2013): Species Differences in the Expression and Activity of Bone Morphogenetic Protein 15. Endocrinology, 154, 888-899  https://doi.org/10.1210/en.2012-2015
Bodin Loys, Di Pasquale Elisa, Fabre Stéphane, Bontoux Martine, Monget Philippe, Persani Luca, Mulsant Philippe (2007): A Novel Mutation in the Bone Morphogenetic Protein 15 Gene Causing Defective Protein Secretion Is Associated with Both Increased Ovulation Rate and Sterility in Lacaune Sheep. Endocrinology, 148, 393-400  https://doi.org/10.1210/en.2006-0764
Crawford J. L., Heath D. A., Reader K. L., Quirke L. D., Hudson N. L., Juengel J. L., McNatty K. P. (): Oocytes in sheep homozygous for a mutation in bone morphogenetic protein receptor 1B express lower mRNA levels of bone morphogenetic protein 15 but not growth differentiation factor 9. Reproduction, 142, 53-61  https://doi.org/10.1530/REP-10-0485
Cui H. X., Zhao S. M., Cheng M. L., Guo L., Ye R. Q., Liu W. Q., Gao S. Z. (): Cloning and Expression Levels of Genes Relating to the Ovulation Rate of the Yunling Black Goat. Biology of Reproduction, 80, 219-226  https://doi.org/10.1095/biolreprod.108.069021
Davis G. H., Montgomery G. W., Allison A. J., Kelly R. W., Bray A. R. (1982): Segregation of a major gene influencing fecundity in progeny of Booroola sheep. New Zealand Journal of Agricultural Research, 25, 525-529  https://doi.org/10.1080/00288233.1982.10425216
Dube Jennifer L., Wang Pei, Elvin Julia, Lyons Karen M., Celeste Anthony J., Matzuk Martin M. (1998): The Bone Morphogenetic Protein 15 Gene Is X-Linked and Expressed in Oocytes. Molecular Endocrinology, 12, 1809-1817  https://doi.org/10.1210/mend.12.12.0206
Fabre S (2003): The Booroola mutation in sheep is associated with an alteration of the bone morphogenetic protein receptor-IB functionality. Journal of Endocrinology, 177, 435-444  https://doi.org/10.1677/joe.0.1770435
Feary E. S, Juengel J. L, Smith P., French M. C, O'Connell A. R, Lawrence S. B, Galloway S. M, Davis G. H, McNatty K. P (): Patterns of Expression of Messenger RNAs Encoding GDF9, BMP15, TGFBR1, BMPR1B, and BMPR2 During Follicular Development and Characterization of Ovarian Follicular Populations in Ewes Carrying the Woodlands FecX2W Mutation. Biology of Reproduction, 77, 990-998  https://doi.org/10.1095/biolreprod.107.062752
Galloway S.M., McNatty K.P., Cambridge L.M., Laitinen M.P., Juengel J.L., Jokiranta T.S., McLaren R.J., Luiro K., Dodds K.G., Montgomery G.W., Beattie A.E., Davis G.H., Ritvos O. (2000): Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner. Nature Genetics, 25, 279–283. https://doi.org/10.1038/77033
Hanrahan J. P. (2003): Mutations in the Genes for Oocyte-Derived Growth Factors GDF9 and BMP15 Are Associated with Both Increased Ovulation Rate and Sterility in Cambridge and Belclare Sheep (Ovis aries). Biology of Reproduction, 70, 900-909  https://doi.org/10.1095/biolreprod.103.023093
Hosoe Misa, Kaneyama Kanako, Ushizawa Koichi, Hayashi Ken-go, Takahashi Toru (2011): Quantitative analysis of bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) gene expression in calf and adult bovine ovaries. Reproductive Biology and Endocrinology, 9, 33-  https://doi.org/10.1186/1477-7827-9-33
Hu D.L., Li Q.F., Xu Y.F., Li E.L., Han Y.D., Tu F., Xie Z. (2010): The tissue expression profile, mRNA expression level and SNPs analysis on GDF9 gene in Hu sheep. Journal of Agricultural Biotechnology, 18, 533–538.
Huang D. W., Wang J. X., Liu Q. Y., Chu M. X., Di R., He J. N., Cao G. L., Fang L., Feng T., Li N. (2013): Analysis on DNA sequence of TSHB gene and its association with reproductive seasonality in goats. Molecular Biology Reports, 40, 1893-1904  https://doi.org/10.1007/s11033-012-2245-0
Juengel J. L. (2002): Growth Differentiation Factor 9 and Bone Morphogenetic Protein 15 Are Essential for Ovarian Follicular Development in Sheep. Biology of Reproduction, 67, 1777-1789  https://doi.org/10.1095/biolreprod.102.007146
Kaivo-Oja Noora, Bondestam Jonas, Kämäräinen Meerit, Koskimies Janne, Vitt Ursula, Cranfield Mark, Vuojolainen Kaisa, Kallio Janne P., Olkkonen Vesa M., Hayashi Masaru, Moustakas Aristidis, Groome Nigel P., ten Dijke Peter, Hsueh Aaron J. W., Ritvos Olli (2003): Growth Differentiation Factor-9 Induces Smad2 Activation and Inhibin B Production in Cultured Human Granulosa-Luteal Cells. The Journal of Clinical Endocrinology & Metabolism, 88, 755-762  https://doi.org/10.1210/jc.2002-021317
Knight Philip G, Glister Claire (2003): Local roles of TGF-β superfamily members in the control of ovarian follicle development. Animal Reproduction Science, 78, 165-183  https://doi.org/10.1016/S0378-4320(03)00089-7
Livak Kenneth J., Schmittgen Thomas D. (2001): Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods, 25, 402-408  https://doi.org/10.1006/meth.2001.1262
Martinez-Royo A., Jurado J. J., Smulders J. P., Martí J. I., Alabart J. L., Roche A., Fantova E., Bodin L., Mulsant P., Serrano M., Folch J., Calvo J. H. (2008): A deletion in the bone morphogenetic protein 15 gene causes sterility and increased prolificacy in Rasa Aragonesa sheep. Animal Genetics, 39, 294-297  https://doi.org/10.1111/j.1365-2052.2008.01707.x
McPherron A.C., Lee S.J. (1993): GDF-3 and GDF-9: two new members of the transforming growth factor-beta superfamily containing a novel pattern of cysteines. Journal of Biological Chemistry, 268, 3444–3449.
Melo E.O., Silva B.D.M., Castro E.A., Silva T.A.S.N., Paiva S.R., Sartori R., Franco M.M., Souza C.J.H, Neves J.P. (2008): A novel mutation in the growth and differentiation factor 9 (GDF9) gene is associated, in homozygosis, with increased ovulation rate in Santa Ines sheep. Biology of Reproduction, 78, 141.
Monteagudo Luis V., Ponz Ricardo, Tejedor M. Teresa, Laviña Adolfo, Sierra Isidro (2009): A 17bp deletion in the Bone Morphogenetic Protein 15 (BMP15) gene is associated to increased prolificacy in the Rasa Aragonesa sheep breed. Animal Reproduction Science, 110, 139-146  https://doi.org/10.1016/j.anireprosci.2008.01.005
Mulsant P., Lecerf F., Fabre S., Schibler L., Monget P., Lanneluc I., Pisselet C., Riquet J., Monniaux D., Callebaut I., Cribiu E., Thimonier J., Teyssier J., Bodin L., Cognie Y., Chitour N., Elsen J.-M. (2001): Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Merino ewes. Proceedings of the National Academy of Sciences, 98, 5104-5109  https://doi.org/10.1073/pnas.091577598
Nicol L., Bishop S. C, Pong-Wong R., Bendixen C., Holm L.-E., Rhind S. M, McNeilly A. S (): Homozygosity for a single base-pair mutation in the oocyte-specific GDF9 gene results in sterility in Thoka sheep. Reproduction, 138, 921-933  https://doi.org/10.1530/REP-09-0193
Otsuka Fumio, Shimasaki Shunichi (2002): A Novel Function of Bone Morphogenetic Protein-15 in the Pituitary: Selective Synthesis and Secretion of FSH by Gonadotropes. Endocrinology, 143, 4938-4941  https://doi.org/10.1210/en.2002-220929
Pramod RK, Sharma SK, Singhi A, Pan S, Mitra A (2013): Differential Ovarian Morphometry and Follicular Expression of BMP15, GDF9 and BMPR1B Influence the Prolificacy in Goat. Reproduction in Domestic Animals, 48, 803-809  https://doi.org/10.1111/rda.12165
Silva B. D. M., Castro E. A., Souza C. J. H., Paiva S. R., Sartori R., Franco M. M., Azevedo H. C., Silva T. A. S. N., Vieira A. M. C., Neves J. P., Melo E. O. (2011): A new polymorphism in the Growth and Differentiation Factor 9 (GDF9) gene is associated with increased ovulation rate and prolificacy in homozygous sheep. Animal Genetics, 42, 89-92  https://doi.org/10.1111/j.1365-2052.2010.02078.x
Silva J.R.V., van den Hurk R., van Tol H.T.A., Roelen B.A.J., Figueiredo J.R. (2005): Expression of growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15), and BMP receptors in the ovaries of goats. Molecular Reproduction and Development, 70, 11-19  https://doi.org/10.1002/mrd.20127
Souza C.J.H., MacDougall C., Campbell B.K., McNeilly A.S., Baird D.T. (2001): The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1B (BMPR1B) gene. Journal of Endocrinology, 169, R1–R6.
Souza C. J. H., McNeilly A. S., Benavides M. V., Melo E. O., Moraes J. C. F. (2014): Mutation in the protease cleavage site of GDF9 increases ovulation rate and litter size in heterozygous ewes and causes infertility in homozygous ewes. Animal Genetics, 45, 732-739  https://doi.org/10.1111/age.12190
Tu Y.R. (1989): The Sheep and Goat Breeds in China. Shanghai Science and Technology Press, Shanghai, China.
Våge Dag I, Husdal Maren, Kent Matthew P, Klemetsdal Gunnar, Boman Inger A (2013): A missense mutation in growth differentiation factor 9 (GDF9) is strongly associated with litter size in sheep. BMC Genetics, 14, 1-  https://doi.org/10.1186/1471-2156-14-1
Wilson T. (2001): Highly Prolific Booroola Sheep Have a Mutation in the Intracellular Kinase Domain of Bone Morphogenetic Protein IB Receptor (ALK-6) That Is Expressed in Both Oocytes and Granulosa Cells. Biology of Reproduction, 64, 1225-1235  https://doi.org/10.1095/biolreprod64.4.1225
Yang Chun-Xian, Zi Xiang-Dong, Wang Yong, Yang Da-Qian, Ma Li, Lu Jian-Yuan, Niu Hui-Ran, Xiao Xiao (2012): Cloning and mRNA expression levels of GDF9, BMP15, and BMPR1B genes in prolific and non-prolific goat breeds. Molecular Reproduction and Development, 79, 2-2  https://doi.org/10.1002/mrd.21386
Yang H., Liu S.R., Zhong F.G., Yang Y.L., Zhang Y.S. (2009): Different expression of BMPR-IB in tissues of sheep. Chinese Journal of Animal Science, 45, 6–8.
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