Concentrations of neopterin, biopterin, and cortisol associated with surgical castration of piglets with lidocainešálek P., Svoboda M., Bernardy J., Večerek V. (2015): Concentrations of neopterin, biopterin, and cortisol associated with surgical castration of piglets with lidocaine. Czech J. Anim. Sci., 60: 473-478.
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The effect of surgical castration with local anesthesia using lidocaine on neopterin, biopterin, and cortisol blood plasma concentrations in piglets was studied. Three groups of 12 piglets were investigated: one group castrated without lidocaine, one group castrated with lidocaine, and one group left as an uncastrated control group handled in the same way as castrated piglets. Blood samples were collected 4 min before castration, and 1 h and 24 h after castration. The time × treatment interaction (P < 0.01) was detected for neopterin concentrations, yielding the result that neopterin was higher (P < 0.01) in castrated piglets without lidocaine 1 h after surgical castration compared with all other groups. The time effect (P < 0.05) was detected for biopterin concentrations. The time × treatment interaction (P < 0.01) was detected for plasma cortisol concentrations, yielding the result that neopterin was higher (P < 0.01) in castrated piglets without lidocaine and in castrated piglets with lidocaine 1 h after surgical castration compared with pre-treatment and concentrations 24 h after surgical castration. The study showed that the use of lidocaine for the surgical castration of piglets may significantly influence the activation of the immune system. This is corroborated by a significant difference in blood plasma neopterin concentrations between piglets castrated with anesthesia and those castrated without it. The use of lidocaine had no effect on cortisol concentrations in comparison with the group castrated without lidocaine.
Atmaca Murad, Kuloglu Murat, Tezcan Ertan, Onal Süleyman, Ustundag Bilal (2002): Neopterin levels and dexamethasone suppression test in posttraumatic stress disorder. European Archives of Psychiatry and Clinical Neuroscience, 252, 161-165
Blahová J., Dobšíková R., Svobodová Z., Kaláb P. (): Simultaneous Determination of Plasma Cortisol by High Pressure Liquid Chromatography and Radioimmunoassay Methods in Fish. Acta Veterinaria Brno, 76, 59-64
Breineková K., Smutná M., Svoboda M., Vorlová L. (2006): Pterins—Possible markers of stress situations in pigs. Toxicology Letters, 164, S160-S161
Breinekova K., Svoboda M., Smutna M., Vorlova L. (2007): Markers of acute stress in pigs. Physiological Research, 56, 323–329.
Brown G.M. (1971): The biosynthesis of pteridines. Advances in Enzymology and Related Areas of Molecular Biology, 35, 35–77.
Carroll J.A., Berg E.L., Strauch T.A., Roberts M.P., Kattesh H.G. (2006): Hormonal profiles, behavioral responses, and short-term growth performance after castration of pigs at three, six, nine, or twelve days of age. Journal of Animal Science, 84, 1271–1278.
Carru Ciriaco, Zinellu Angelo, Sotgia Salvatore, Serra Roberta, Usai Maria Franca, Pintus Gian Franco, Pes Giovanni Mario, Deiana Luca (2004): A new HPLC method for serum neopterin measurement and relationships with plasma thiols levels in healthy subjects. Biomedical Chromatography, 18, 360-366
Fujioka Hiroki, Shintaku Haruo, Nakanishi Hidehiko, Kim Tae-Jang, Kusuda Satoshi, Yamano Tsunekazu (2008): Biopterin in the acute phase of hypoxia–ischemia in a neonatal pig model. Brain and Development, 30, 1-6
Hoffmann G., Wirleitner B., Fuchs D. (2003): Potential role of immune system activation-associated production of neopterin derivatives in humans. Inflammation Research, 52, 313-321
Jacobson M., Lindberg J.E., Lindberg R., Segerstad C.H., Wallgren P., Fellstrom C., Hulten C., Jensen-Waern M. (2001): Intestinal cannulation: model for study of the midgut of the pig. Comparative Medicine, 51, 163–170.
Kluivers-Poodt M., Houx B. B., Robben S. R. M., Koop G., Lambooij E., Hellebrekers L. J. (2012): Effects of a local anaesthetic and NSAID in castration of piglets, on the acute pain responses, growth and mortality. animal, 6, 1469-1475
Marsalek P., Svoboda M., Smutna M., Blahova J., Vecerek V. (): Neopterin and biopterin as biomarkers of immune system activation associated with castration in piglets. Journal of Animal Science, 89, 1758-1762
Llamas Moya Sara, Boyle Laura A., Lynch Patrick Brendan, Arkins Sean (2008): Effect of surgical castration on the behavioural and acute phase responses of 5-day-old piglets. Applied Animal Behaviour Science, 111, 133-145
Petersen Henrik Hagbard, Nielsen Jens Peter, Heegaard Peter Mikael Helweg (2004): Application of acute phase protein measurements in veterinary clinical chemistry. Veterinary Research, 35, 163-187
Prunier A., Mounier A.M., Hay M. (2005): Effects of castration, tooth resection, or tail docking on plasma metabolites and stress hormones in young pigs. Journal of Animal Science, 83, 216–222.
RANHEIM B., HAGA H. A., INGEBRIGTSEN K. (2005): Distribution of radioactive lidocaine injected into the testes in piglets. Journal of Veterinary Pharmacology and Therapeutics, 28, 481-483
Salak-Johnson J. L., McGlone J. J. (2007): Making sense of apparently conflicting data: Stress and immunity in swine and cattle. Journal of Animal Science, 85, E81-E88
Satas S., Johannessen S.I., Hoem N.O., Haaland K., Sorensen D.R., Thoresen M. (1997): Lidocaine pharmacokinetics and toxicity in newborn pigs. Anesthesia and Analgesia, 85, 306–312.
Singi G., Garcia K.C., Coelho A.E.E., Gazola R. (2001): Norepinephrine prevents the adverse effects of lidocaine upon the heart. An experimental study in isolated guinea-pig hearts. Pharmacological Research, 44, 129-134
Sjaastad O., Hove K., Sand O. (2003): The nervous system. Regulation of body temperature. In: Steel C. (ed.): Physiology of Domestic Animals. Scandinavian Veterinary Press, Oslo, Norway, 95–146.
Smutna Miriam, Svoboda Martin, Breinekova Klara (2010): Pterins as Sensors of Response to the Application of Fe3+-Dextran in Piglets. Sensors, 10, 890-900
Werner-Felmayer G., Golderer G., Werner E. (2002): Tetrahydrobiopterin Biosynthesis, Utilization and Pharmacological Effects. Current Drug Metabolism, 3, 159-173
White R.G., Deshazer J.A., Tressler C.J., Borcher G.M., Davey S., Waninge A., Parkhurst A.M., Milanuk M.J., Clemens E.T. (1995): Vocalization and physiological response of pigs during castration with or without a local anesthetic. Journal of Animal Science, 73, 381–386.
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