Application of mtDNA markers for European huchen (Hucho hucho Linnaeus, 1758) management in Poland

https://doi.org/10.17221/8599-CJASCitation:Kucinski M., Fopp-Bayat D., Zivna D., Liszewski T., Svinger V., Lebeda I. (2015): Application of mtDNA markers for European huchen (Hucho hucho Linnaeus, 1758) management in Poland. Czech J. Anim. Sci., 60: 564-569.
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Four broodstocks of European huchen (Hucho hucho) from Poland, Germany, Slovakia, and Ukraine were investigated using five selected fragments of mtDNA. The mitochondrial DNA sequence analysis was successfully applied to the Polish and German broodstocks of European huchen for the first time. A very low haplotype (= 0.097) and nucleotide (π = 0.00013) diversity across 3573 bases of mtDNA fragments (partial regions of NADH-1, NADH-5, ATPase 6, Cytochrome b, and D-loop) evidenced by three closely related mtDNA haplotypes were found. The analysis of pairwise genetic differentiation (Fst) displayed a statistically significant differentiation between German (clade A) and the rest of examined broodstocks (clade B), clustering them into two separate groups. Moreover, the applied mtDNA markers did not reveal any differences among fish from the clade B, suggesting that other markers should be used to display a deeper genetic background of the studied broodstocks. The two clusters of European huchen distinguished under the current study should be considered as distinct management units by managers, who may be tempted to transport brood fish or yearlings across the range of European huchen distribution. It means that stocking material and spawners of European huchen from the upper parts of the Danube River (Clade A) should not be mixed with those from the lower parts (Clade B). Moreover, for any supplementation of broodstocks in order to increase their genetic variability only the fishes within the described management units should be used.
References:
Balakirev Evgeniy S., Romanov Nikolai S., Mikheev Pavel B., Ayala Francisco J., Barendse William (2013): Mitochondrial DNA Variation and Introgression in Siberian Taimen Hucho taimen. PLoS ONE, 8, e71147-  https://doi.org/10.1371/journal.pone.0071147
 
Balloux Francois, Lugon-Moulin Nicolas (2002): The estimation of population differentiation with microsatellite markers. Molecular Ecology, 11, 155-165  https://doi.org/10.1046/j.0962-1083.2001.01436.x
 
Crespi Bernard J, Fulton Michael J (2004): Molecular systematics of Salmonidae: combined nuclear data yields a robust phylogeny. Molecular Phylogenetics and Evolution, 31, 658-679  https://doi.org/10.1016/j.ympev.2003.08.012
 
Frankham R., Briscoe D.A., Ballou J.D. (eds) (2002): Introduction to Conservation Genetics. Cambridge University Press, Cambridge, UK.
 
Froufe E., Alekseyev S., Knizhin I., Alexandrino P., Weiss S. (2003): Comparative phylogeography of salmonid fishes (Salmonidae) reveals late to post-Pleistocene exchange between three now-disjunct river basins in Siberia. Diversity <html_ent glyph="@amp;" ascii="&amp;"/> Distributions, 9, 269-282  https://doi.org/10.1046/j.1472-4642.2003.00024.x
 
Froufe E., Alekseyev S., Knizhin I., Weiss S. (2005): Comparative mtDNA sequence (control region, ATPase 6 and NADH-1) divergence in Hucho taimen(Pallas) across four Siberian river basins. Journal of Fish Biology, 67, 1040-1053  https://doi.org/10.1111/j.0022-1112.2005.00807.x
 
Geist Juergen (2011): Integrative freshwater ecology and biodiversity conservation. Ecological Indicators, 11, 1507-1516  https://doi.org/10.1016/j.ecolind.2011.04.002
 
Geist J., Kolahsa M., Gum B., Kuehn R. (2009): The importance of genetic cluster recognition for the conservation of migratory fish species: the example of the endangered European huchen Hucho hucho (L.). Journal of Fish Biology, 75, 1063-1078  https://doi.org/10.1111/j.1095-8649.2009.02377.x
 
Gillooly J. F., Allen A. P., West G. B., Brown J. H. (): The rate of DNA evolution: Effects of body size and temperature on the molecular clock. Proceedings of the National Academy of Sciences, 102, 140-145  https://doi.org/10.1073/pnas.0407735101
 
GIUFFRA E., BERNATCHEZ L., GUYOMARD R. (1994): Mitochondrial control region and protein coding genes sequence variation among phenotypic forms of brown trout Salmo trutta from northern Italy. Molecular Ecology, 3, 161-171  https://doi.org/10.1111/j.1365-294X.1994.tb00117.x
 
HUFF DAVID D., MILLER LOREN M., CHIZINSKI CHRISTOPHER J., VONDRACEK BRUCE (2011): Mixed-source reintroductions lead to outbreeding depression in second-generation descendents of a native North American fish. Molecular Ecology, 20, 4246-4258  https://doi.org/10.1111/j.1365-294X.2011.05271.x
 
Kucinski M., Fopp-Bayat D., Liszewski T., Svinger V. W., Lebeda I., Kolman R. (2015): Genetic analysis of four European huchen (Hucho hucho Linnaeus, 1758) broodstocks from Poland, Germany, Slovakia, and Ukraine: implication for conservation. Journal of Applied Genetics, 56, 469-480  https://doi.org/10.1007/s13353-015-0274-9
 
Liu Haixia, Li Yang, Liu Xiaolin, Xiong Dongmei, Wang Lixin, Zou Guiwei, Wei Qiwei (2015): Phylogeographic structure of Brachymystax lenok tsinlingensis (Salmonidae) populations in the Qinling Mountains, Shaanxi, based on mtDNA control region. Mitochondrial DNA, 26, 532-537  https://doi.org/10.3109/19401736.2013.865168
 
Marić Saša, Razpet Andrej, Nikolić Vera, Simonović Predrag (2011): Genetic differentiation of European grayling (Thymallus thymallus) populations in Serbia, based on mitochondrial and nuclear DNA analyses. Genetics Selection Evolution, 43, 2-  https://doi.org/10.1186/1297-9686-43-2
 
Maric S., Alekseyev S., Snoj A., Askeyev O., Akseyev I., Weiss S. (2014a): First mrDNA sequencing of Volga and Ob basin taimen Hucho taimen: European populations stem from a late Pleistocene expansion of H. taimen out of western Siberia and are not intermediate to Hucho hucho. Journal of Fish Biology, 85, 530–539.
 
Maric S., Razpet A., Nikolic V., Snoj A., Simonovic P. (2014b): Analysis of genetic structure of huchen (Hucho hucho) in Serbia inferred from mitochondrial and nuclear DNA. Acta Veterinaria – Beograd, 64, 236–244.
 
Oleinik A. G., Skurikhina L. A. (2008): Phylogenetic relationships of Sakhalin taimen Parahucho perryi inferred from PCR-RFLP analysis of mitochondrial DNA. Russian Journal of Genetics, 44, 767-776  https://doi.org/10.1134/S102279540807003X
 
Razpet A., Sušnik S., Jug T., Snoj A. (2007): Genetic variation among trout in the River Neretva basin, Bosnia and Herzegovina. Journal of Fish Biology, 70, 94-110  https://doi.org/10.1111/j.1095-8649.2007.01392.x
 
Shedko S. V., Miroshnichenko I. L., Nemkova G. A. (2013): Phylogeny of salmonids (salmoniformes: Salmonidae) and its molecular dating: Analysis of mtDNA data. Russian Journal of Genetics, 49, 623-637  https://doi.org/10.1134/S1022795413060112
 
Snoj Aleš, Marić Saša, Berrebi Patrick, Crivelli Alain J, Shumka Spase, Sušnik Simona (2009): Genetic architecture of trout from Albania as revealed by mtDNA control region variation. Genetics Selection Evolution, 41, 22-  https://doi.org/10.1186/1297-9686-41-22
 
Sunnucks Paul (2000): Efficient genetic markers for population biology. Trends in Ecology & Evolution, 15, 199-203  https://doi.org/10.1016/S0169-5347(00)01825-5
 
Verspoor E, O’Sullivan M, Arnold A L, Knox D, Amiro P G (): Restricted matrilineal gene flow and regional differentiation among Atlantic salmon (Salmo salar L.) populations within the Bay of Fundy, eastern Canada. Heredity, 89, 465-472  https://doi.org/10.1038/sj.hdy.6800166
 
Wang Ying, Guo Rui, Li Hua, Zhang Xiuyue, Du Jun, Song Zhaobin (2011): The complete mitochondrial genome of the Sichuan taimen (Hucho bleekeri): Repetitive sequences in the control region and phylogenetic implications for Salmonidae. Marine Genomics, 4, 221-228  https://doi.org/10.1016/j.margen.2011.06.003
 
Weiss S., Marić S., Snoj A. (2011): Regional structure despite limited mtDNA sequence diversity found in the endangered Huchen, Hucho hucho (Linnaeus, 1758). Hydrobiologia, 658, 103-110  https://doi.org/10.1007/s10750-010-0453-y
 
Witkowski A., Bajic A., Treer T., Hegedis A., Maric S., Sprem N., Piria M., Kapusta A. (2013a): Past and present of and perspectives for the Danube huchen, Hucho hucho (L.), in the Danube basin. Archives of Polish Fisheries, 21, 129–142.
 
Witkowski A., Goryczko K., Kowalewski M. (2013b): The history of huchen, Hucho hucho (L.), in Poland – distribution, restoration and conservation. Archives of Polish Fisheries, 21, 161–168.
 
Wolf Christian, Hübner Philipp, Lüthy Jürg (1999): Differentiation of sturgeon species by PCR-RFLP. Food Research International, 32, 699-705  https://doi.org/10.1016/S0963-9969(99)00150-7
 
Wu Xue-Chang (2006): The Loss of Genetic Diversity in Sichuan Taimen as Revealed by DNA Fingerprinting. Biochemical Genetics, 44, 177-185  https://doi.org/10.1007/s10528-006-9021-6
 
Xia Y.Z., Chen Y.Y., Sheng Y. (2006): Phylogeographic structure of lenok (Brachymystax lenok Pallas) (Salmoninae, Salmonidae) populations in water systems of eastern China, inferred from mitochondrial DNA sequences. Zoological Studies, 45, 190–200.
 
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