Association of the ADRB3, FABP3, LIPE, and LPL gene polymorphisms with pig intramuscular fat content and fatty acid composition

https://doi.org/10.17221/7975-CJASCitation:Xue W., Wang W., Jin B., Zhang X., Xu X. (2015): Association of the ADRB3, FABP3, LIPE, and LPL gene polymorphisms with pig intramuscular fat content and fatty acid composition. Czech J. Anim. Sci., 60: 60-66.
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The aim of the present study was to investigate the associations of single nucleotide polymorphisms (SNPs) in candidate genes with fatness traits in the Longissimus dorsi muscle of pigs. The polymorphisms of genes were investigated, which included beta-3-adrenergic receptor gene (ADRB3), heart fatty acid-binding protein gene (FABP3), and hormone-sensitive lipase gene (LIPE) as well as lipoprotein lipase gene (LPL). The intramuscular fat (IMF) content and fatty acid composition contents in Longissimus dorsi muscle samples were measured. Results showed that ADRB3, LIPE, and LPL SNPs were associated with IMF content (P < 0.05). ADRB3 AG heterozygotes exhibited higher IMF content. LIPE A allele was associated with greater IMF content. LPL CT heterozygotes exhibited the lowest IMF content. ADRB3 c.1192G>A had highly significant association with the total monounsaturated fatty acid (MUFA) (P < 0.01) and the total polyunsaturated fatty acid (PUFA) (P < 0.01). LIPE c.442G>A was significantly associated with the contents of C12:0 and C14:0 (P < 0.05). LPL c.624C>T was significantly associated with the percentage of C16:1 (P < 0.05) and the percentage of total saturated fatty acid (SFA) (P < 0.05). The pigs with ADRB3 G allele had more MUFA, and the pigs with LPL T allele had less SFA, implying that the ADRB3 G and LPL T in pigs may be beneficial to human health. In conclusion, the results suggest that these genetic markers are important sources of the variations for the pork selection to obtain favourable meat with higher IMF levels and appropriate fatty acid composition.  
References:
Chmurzyńska Agata (2006): The multigene family of fatty acid-binding proteins (FABPs): Function, structure and polymorphism. Journal of Applied Genetics, 47, 39-48  https://doi.org/10.1007/BF03194597
 
Cieslak Jakub, Nowacka-Woszuk Joanna, Bartz Magdalena, Fijak-Nowak Honorata, Grzes Maria, Szydlowski Maciej, Switonski Marek (2009): Association studies on the porcine RETN, UCP1, UCP3 and ADRB3 genes polymorphism with fatness traits. Meat Science, 83, 551-554  https://doi.org/10.1016/j.meatsci.2009.07.001
 
Fortin A., Robertson W.M., Tong A.K.W. (2005): The eating quality of Canadian pork and its relationship with intramuscular fat. Meat Science, 69, 297-305  https://doi.org/10.1016/j.meatsci.2004.07.011
 
Gasser Robin B, Hu Min, Chilton Neil B, Campbell Bronwyn E, Jex Aaron J, Otranto Domenico, Cafarchia Claudia, Beveridge Ian, Zhu Xingquan (2007): Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation. Nature Protocols, 1, 3121-3128  https://doi.org/10.1038/nprot.2006.485
 
Gerbens Frans, Rettenberger Günther, Lenstra Johannes A., Veerkamp Jacques H., Pas Marinus F. W. te (1997): Characterization, chromosomal localization, and genetic variation of the porcine heart fatty acid-binding protein gene. Mammalian Genome, 8, 328-332  https://doi.org/10.1007/s003359900433
 
Gu F., Harbitz I., Chowdhary B.P., Davies W., Gustavsson I. (1992): Mapping of the porcine lipoprotein lipase (LPL) gene to chromosome 14q12&rarr;q14 by in situ hybridization. Cytogenetic and Genome Research, 59, 63-64  https://doi.org/10.1159/000133201
 
HIROSE Kensuke, NAKAMURA Mariko, TAKIZAWA Tatsuya, FUKAWA Kazuo, ITO Tetsuya, UEDA Masayuki, SASAKI Takashi, TANAKA Kazuaki (2009): An insertion/deletion variant of a thymine base in exon 2 of the porcine beta 3-adrenergic receptor gene associated with loin eye muscle area. Animal Science Journal, 80, 624-630  https://doi.org/10.1111/j.1740-0929.2009.00686.x
 
Horrell A., Forrest R. H. J., Zhou H., Fang Q., Hickford J. G. H. (2009): Association of the ADRB3 gene with birth weight and growth rate to weaning in New Zealand Romney sheep. Animal Genetics, 40, 251-251  https://doi.org/10.1111/j.1365-2052.2008.01807.x
 
Klensporf-Pawlik D., Szydlowski M., Kaczmarek A., Nowacka-Woszuk J., Switonski M., Jelen H. (2012): The fatty acid composition of the Longissimus dorsi muscle, subcutaneous and visceral fats differ in four commercial pig breeds. Journal of Animal and Feed Sciences, 21, 661–676.
 
Knoll A., Stratil A., Nebola M., Cepica S. (1998): Characterization of a polymorphism in exon 1 of the porcine hormone-sensitive lipase (LIPE) gene. Animal Genetics, 29, 462–463.
 
Large V., Reynisdottir S., Langin D., Fredby K., Klannemark M., Holm C., Arner P. (1999): Decreased expression and function of adipocyte hormone-sensitive lipase in subcutaneous fat cells of obese subjects. Journal of Lipid Research, 40, 2059–2066.
 
Lee S.H., Choi Y.M., Choe J.H., Kim J.M., Hong K.C., Park H.C., Kim B.C. (2010): Association between polymorphisms of the heart fatty acid binding protein gene and intramuscular fat content, fatty acid composition, and meat quality in Berkshire breed. Meat Science, 86, 794-800  https://doi.org/10.1016/j.meatsci.2010.06.024
 
Luo He-Feng, Wei Hong-Kui, Huang Fei-Ruo, Zhou Zheng, Jiang Si-Wen, Peng Jian (2009): The Effect of Linseed on Intramuscular Fat Content and Adipogenesis Related Genes in Skeletal Muscle of Pigs. Lipids, 44, 999-1010  https://doi.org/10.1007/s11745-009-3346-y
 
Mellink C.H.M., Lahbib-Mansais Y., Yerle M., Gellin J. (1993): Localization of four new markers to pig chromosomes 1 6, and 14 by radioactive in situ hybridization. Cytogenetic and Genome Research, 64, 256-260  https://doi.org/10.1159/000133588
 
Mersmann H.J. (1998): Lipoprotein and hormone-sensitive lipases in porcine adipose tissue. Journal of Animal Science, 76, 1396–1404.
 
Miyoshi Hideaki, Perfield James W., Obin Martin S., Greenberg Andrew S. (2008): Adipose triglyceride lipase regulates basal lipolysis and lipid droplet size in adipocytes. Journal of Cellular Biochemistry, 105, 1430-1436  https://doi.org/10.1002/jcb.21964
 
Nowacka-Woszuk Joanna, Szczerbal Izabela, Fijak-Nowak Honorata, Switonski Marek (2008): Chromosomal localization of 13 candidate genes for human obesity in the pig genome. Journal of Applied Genetics, 49, 373-377  https://doi.org/10.1007/BF03195636
 
Raj St., Skiba G., Weremko D., Fandrejewski H., Migdał W., Borowiec F., Poławska E. (2010): The relationship between the chemical composition of the carcass and the fatty acid composition of intramuscular fat and backfat of several pig breeds slaughtered at different weights. Meat Science, 86, 324-330  https://doi.org/10.1016/j.meatsci.2010.04.037
 
Reardon W., Mullen A.M., Sweeney T., Hamill R.M. (2010): Association of polymorphisms in candidate genes with colour, water-holding capacity, and composition traits in bovine M. longissimus and M. semimembranosus. Meat Science, 86, 270-275  https://doi.org/10.1016/j.meatsci.2010.04.013
 
Schwab C. R., Baas T. J., Stalder K. J., Mabry J. W. (2007): Deposition rates and accretion patterns of intramuscular fat, loin muscle area, and backfat of Duroc pigs sired by boars from two time periods. Journal of Animal Science, 85, 1540-1546  https://doi.org/10.2527/jas.2006-343
 
Tao X., Men X.M., Deng B., Xu Z.W. (2013): Effects of breed, postnatal development, and nutrition on mRNA expression of the FTO gene in porcine muscle and its relationship with intramuscular fat deposition. Czech Journal of Animal Science, 58, 381–388.
 
Tyra M., Ropka-Molik K. (2011): Effect of the FABP3 and LEPR gene polymorphisms and expression levels on intramuscular fat (IMF) content and fat cover degree in pigs. Livestock Science, 142, 114-120  https://doi.org/10.1016/j.livsci.2011.07.003
 
Wang Wei, Xue Wenda, Jin Bangquan, Zhang Xixia, Ma Fei, Xu Xiaofeng (2013): Candidate gene expression affects intramuscular fat content and fatty acid composition in pigs. Journal of Applied Genetics, 54, 113-118  https://doi.org/10.1007/s13353-012-0131-z
 
Wang Y., Xie Y.T., Liu D. (2009): Molecular cloning and mutation site analysis of LPL gene in swine. Chinese Agricultural Science Bulletin, 25, 13–16.
 
Wood J. D., Enser M., Fisher A. V., Nute G. R., Richardson R. I., Sheard P. R. (1999): Manipulating meat quality and composition. Proceedings of the Nutrition Society, 58, 363-370  https://doi.org/10.1017/S0029665199000488
 
Wood J.D, Richardson R.I, Nute G.R, Fisher A.V, Campo M.M, Kasapidou E, Sheard P.R, Enser M (2004): Effects of fatty acids on meat quality: a review. Meat Science, 66, 21-32  https://doi.org/10.1016/S0309-1740(03)00022-6
 
Zechner R., Kienesberger P. C., Haemmerle G., Zimmermann R., Lass A. (2008): Adipose triglyceride lipase and the lipolytic catabolism of cellular fat stores. The Journal of Lipid Research, 50, 3-21  https://doi.org/10.1194/jlr.R800031-JLR200
 
Zhang S., Knight T.J., Stalder K.J., Goodwin R.N., Lonergan S.M., Beitz D.C. (2009): Effects of breed, sex and halothane genotype on fatty acid composition of triacylglycerols and phospholipids in pork longissimus muscle. Journal of Animal Breeding and Genetics, 126, 259-268  https://doi.org/10.1111/j.1439-0388.2008.00782.x
 
Zhao S. M., Ren L. J., Chen L., Zhang X., Cheng M. L., Li W. Z., Zhang Y. Y., Gao S. Z. (2009): Differential Expression of Lipid Metabolism Related Genes in Porcine Muscle Tissue Leading to Different Intramuscular Fat Deposition. Lipids, 44, 1029-1037  https://doi.org/10.1007/s11745-009-3356-9
 
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