Preservation effects of melatonin on the quality and fertility of native Fars rooster semen during liquid storage M., Shahneh A.Z., Zamiri M.J., Zeinoaldini S., Kohram H., Hashemi M.R., Asghari S. (2016): Preservation effects of melatonin on the quality and fertility of native Fars rooster semen during liquid storage. Czech J. Anim. Sci., 61: 42-48.
download PDF
Liquid or frozen storage of poultry semen negatively affects the sperm motility and has crucial role in reducing fertility. Phospholipids in chicken sperm membrane are composed of high proportions of polyunsaturated fatty acids which are susceptible to lipid peroxidation. One of possible ways to improve semen quality is supplementing the ration with antioxidant compounds such as melatonin. In this study, seventy-two roosters were randomly divided into three equal groups. The first group (control; Group C) was exposed to 14 h light and 10 h darkness. The second group (Group M) was exposed to the same lighting period, but in combination with melatonion supplementation (3 mg/kg body weight daily). The third group (Group L) was exposed to 24 h constant light. Semen was collected using abdominal massage and stored for 6 and 12 h at 5°C. Motility, viability, malondialdehyde (MDA) concentration, fertility, and hatchability were evaluated before and after storage. The results showed that the percentage of viable and motile sperms was significantly (P < 0.05) decreased in the control samples after 6 h storage but in Group M these parameters were significantly (P < 0.05) decreased after 12 h. The MDA concentration was significantly (P < 0.05) decreased in Group M compared with Group C after 6 and 12 h storage. The sperm membrane lipid analysis showed that the percentage of unsaturated fatty acids in Group M was significantly (P < 0.05) lower than in Group C. Fertility and hatchability did not change significantly (P < 0.05) both in M and L Groups compared with C Group. Melatonin administration improved semen quality and decreased lipid peroxidation during liquid storage. It also reduced the percentage of polyunsaturated fatty acids in the sperm membrane lipid composition but it did not affect fertility and hatchability.
chicken sperm; antioxidant; lipid peroxidation
Bajpai P. K. (1963): The Effect of Photoperiodicity on Semen Characteristics of Poultry. Poultry Science, 42, 462-465
Bakst M. R. (1980): Fertilizing capacity and morphology of fowl and turkey spermatozoa in hypotonic extender. Reproduction, 60, 121-127
Blanco J. M. (2000): Species Variation in Osmotic, Cryoprotectant, and Cooling Rate Tolerance in Poultry, Eagle, and Peregrine Falcon Spermatozoa. Biology of Reproduction, 63, 1164-1171
Bréque Christelle, Surai Peter, Brillard Jean-Pierre (2003): Roles of antioxidants on prolonged storage of avian spermatozoa in vivo and in vitro. Molecular Reproduction and Development, 66, 314-323
Burrows W., Quinns J. (1936): The collection of spermatozoa from the domestic fowl and turkey. Poultry Science, 40, 8–10.
Cerolini S., Surai P., Maldjian A., Gliozzi T., Noble R. (1997): Lipid composition of semen in different fowl breeders. Avian Poultry and Biology Reviews, 8, 141–148.
Cerolini S, Maldjian A, Surai P, Noble R (2000): Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage. Animal Reproduction Science, 58, 99-111
Cerolini S., Pizzi F., Gliozzi T., Maldjian A., Zaniboni L., Parodi L. (2003): Lipid manipulation of chicken semen by dietary means and its relation to fertility: A review. World's Poultry Science Journal, 59, 65-75
Cerolini S., Zaniboni L., Maldjian A., Gliozzi T. (2006): Effect of docosahexaenoic acid and α-tocopherol enrichment in chicken sperm on semen quality, sperm lipid composition and susceptibility to peroxidation. Theriogenology, 66, 877-886
Cheung Kenneth M. C., Lu D. S., Poon Angela M. S., Wang T., Luk Keith D. K., Leong John C. Y. (2003): Effect of Melatonin Suppression on Scoliosis Development in Chickens by Either Constant Light or Surgical Pinealectomy. Spine, 28, 1941-1944
Christie W.W. (1982): Isolation of lipids from tissues. In: Christie W.W. (ed.): Lipid Analysis: Isolation, Separation, Identification and StructuralAnalysis of Lipids. Pergamon Press, Oxford, UK, 17–25.
Christie W. W., Noble R. C., Moore J. H. (1970): Determination of lipid classes by a gas-chromatographic procedure. The Analyst, 95, 940-
Esterbauer M., Cheeseman K.H. (1990): Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods in Enzymology, 186, 407–421.
Guerrero Juan, Reiter Russel (2002): Melatonin-Immune System Relationships. Current Topics in Medicinal Chemistry, 2, 167-179
Maladgian A., Cerolini S., Surai P., Speak B.K. (1998): The effect of vitamin E, green tea extracts and catechin on in vitro storage of turkey spermatozoa at room temperature. Avian Poultry and Biology Reviews, 9, 143–151.
Mayo J. C., Sainz R. M., Antolín I., Herrera F., Martin V., Rodriguez C. (2002): Melatonin regulation of antioxidant enzyme gene expression. Cellular and Molecular Life Sciences, 59, 1706-1713
NISSEN H.P., KREYSEL H.W. (1983): Polyunsaturated Fatty Acids in Relation to Sperm Motility. Andrologia, 15, 264-269
Ollero M., Alvarez J.G. (2003): Fatty acid remodelling during sperm maturation: variation of docosahexaenoic acid content. In: DeVriese S., Christophe A.B. (eds): Male Fertility and Lipid Metabolism. AOCS Press, Champaign, USA, 23–40.
Ramadan T.A., Taha T.A., Samak M.A., Hassan A. (2009): Effectiveness of exposure to longday followed by melatonin treatment on semen characteristics of Damascus male goats during breeding and non-breeding seasons. Theriogenology, 71, 458-468
REITER R. J. (1980): The Pineal and Its Hormones in the Control of Reproduction in Mammals. Endocrine Reviews, 1, 109-131
Reiter Russel J., Tan Dun-xian, Osuna Carmen, Gitto Eloisa (2000): Actions of melatonin in the reduction of oxidative stress. Journal of Biomedical Science, 7, 444-458
Reiter R.J., Tan D.X., Mayo J.C., Sainz R.M., Leon J., Czarnocki Z. (2003): Melatonin as an antioxidant: biochemical mechanisms and pathophysiological implications in humans. Acta Biochimica Polonica, 50, 1129–1146.
Rodriguez Carmen, Mayo Juan C., Sainz Rosa M., Antolin Isaac, Herrera Federico, Martin Vanesa, Reiter Russel J. (2004): Regulation of antioxidant enzymes: a significant role for melatonin. Journal of Pineal Research, 36, 1-9
SEXTON T. J., GIESEN A. F. (1982): Beltsville Poultry Semen Extender: 6. Holding Turkey Semen for Six Hours at 15 C. Poultry Science, 61, 1202-1208
Surai P. (1999): Vitamin E in avian reproduction. Avian Poultry and Biology Reviews, 10, 1–6.
Surai P. F., Kutz E., Wishart G. J., Noble R. C., Speake B. K. (1997): The relationship between the dietary provision of  -tocopherol and the concentration of this vitamin in the semen of chicken: effects on lipid composition and susceptibility to peroxidation. Reproduction, 110, 47-51
Tan Dun-xian, Reiter Russel, Manchester Lucien, Yan Mei-ting, El-Sawi Mamdouh, Sainz Rosa, Mayo Juan, Kohen Ron, Allegra Mario, Hardelan Rudiger (2002): Chemical and Physical Properties and Potential Mechanisms: Melatonin as a Broad Spectrum Antioxidant and Free Radical Scavenger. Current Topics in Medicinal Chemistry, 2, 181-197
Zaniboni Luisa, Cerolini Silvia (2009): Liquid storage of turkey semen: Changes in quality parameters, lipid composition and susceptibility to induced in vitro peroxidation in control, n-3 fatty acids and alpha-tocopherol rich spermatozoa. Animal Reproduction Science, 112, 51-65
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti