Identification of Smallanthus sonchifolius in herbal tea mixtures by PCR and DART/TOF-MS methodsŽiarovská J., Rajchl A., Fernández E., Prchalová J., Milella L. (2016): Identification of Smallanthus sonchifolius in herbal tea mixtures by PCR and DART/TOF-MS methods. Czech J. Food Sci., 34: 495-502.
download PDF
The identification of yacon, a medicinal plant, in tea mixtures by rapid Polymerase Chain Reaction (PCR) and the Direct Analysis in Real Time coupled with Time-of-Flight Mass Spectrometry (DART/TOF-MS) method were evaluated. Three tea products and a pure yacon tea were analysed using the molecular method PCR, concretely the intraspecific variation of the internal transcribed spacer (ITS) regions of rDNA and the DART method coupled with TOF-MS. The results show the reliability of PCR and restriction cleavage of the ITS as a combined approach to confirm the presence of yacon in herbal tea mixtures. Three fragments of approximately 700, 408, and 235 bp in length are present when yacon is a part of the herbal tea mixture. The Principal Component Analysis (PCA) based on the fingerprints of the complete Total Ion Current (TIC) mass spectra shows sufficient separation of herbal teas with and without yacon leaves. The reported methods are technically rapid and can be used as an effective tool for the purposes of yacon identification or authentication.
Al-Balaa Dania, Rajchl Aleš, Grégrová Adéla, Ševčík Rudolf, Čížková Helena (2014): DART mass spectrometry for rapid screening and quantitative determination of cholesterol in egg pasta. Journal of Mass Spectrometry, 49, 911-917
Aybar Manuel J, Sánchez Riera Alicia N, Grau Alfredo, Sánchez Sara S (2001): Hypoglycemic effect of the water extract of Smallantus sonchifolius (yacon) leaves in normal and diabetic rats. Journal of Ethnopharmacology, 74, 125-132
Čajka T., Václavík L., Riddellová K., Hajslová J. (2008): GC-TOF-MS and DART-TOF-MS: Challenges in the analysis of soft drinks. LC GC Europe, 21: 250–256.
Clements Mark (2003): Molecular phylogenetic systematics in the Dendrobiinae (Orchidaceae), with emphasis on Dendrobium sect. Pedilonum. Telopea, 10, 247-298
Chen H., Gamez G., Zenobi R. (2009): What can we learn from ambient ionization techniques? Journal of American Society of Mass Spectrometry, 20: 1947–1963.
Deng Jiewei, Yang Yunyun (2013): Chemical fingerprint analysis for quality assessment and control of Bansha herbal tea using paper spray mass spectrometry. Analytica Chimica Acta, 785, 82-90
Espiñeira Montserrat, González-Lavín Nerea, Vieites Juan M., Santaclara Francisco J. (2008): Authentication of Anglerfish Species (Lophius spp) by Means of Polymerase Chain Reaction−Restriction Fragment Length Polymorphism (PCR−RFLP) and Forensically Informative Nucleotide Sequencing (FINS) Methodologies. Journal of Agricultural and Food Chemistry, 56, 10594-10599
Espiñeira M., Herrero B., Vieites J.M., Santaclara F.J. (2010): Detection and identification of anisakids in seafood by fragment length polymorphism analysis and PCR–RFLP of ITS-1 region. Food Control, 21, 1051-1060
Fernández E.C., Viehmannová I., Lachman J., Milella L. (2006): Yacon [Smallanthus sonchifolius (Poeppig & Endlicher) H. Robinson]: a new crop in the Central Europe. Plant Soil and Environment, 52: 564–570.
Fernández Eloy Cusimamani, Rajchl Aleš, Lachman Jaromír, Čížková Helena, Kvasnička František, Kotíková Zora, Milella Luigi, Voldřich Michal (2013): Impact of yacon landraces cultivated in the Czech Republic and their ploidy on the short- and long-chain fructooligosaccharides content in tuberous roots. LWT - Food Science and Technology, 54, 80-86
Fraser Karl, Lane Geoff A., Otter Don E., Harrison Scott J., Quek Siew-Young, Hemar Yacine, Rasmussen Susanne (2013): Monitoring tea fermentation/manufacturing by direct analysis in real time (DART) mass spectrometry. Food Chemistry, 141, 2060-2065
Fukai Katsuhiko, Miyazaki Sachiko, Nanjo Fumio, Hara Yukihiko (1993): Distribution of carbohydrates and related enzyme activities in yacon (polymnia sonchifolia). Soil Science and Plant Nutrition, 39, 567-571
Ganopoulos Ioannis, Madesis Panagiotis, Tsaftaris Athanasios (2012): Universal ITS2 Barcoding DNA Region Coupled with High-Resolution Melting (HRM) Analysis for Seed Authentication and Adulteration Testing in Leguminous Forage and Pasture Species. Plant Molecular Biology Reporter, 30, 1322-1328
Gao Ting, Yao Hui, Song Jingyuan, Liu Chang, Zhu Yingjie, Ma Xinye, Pang Xiaohui, Xu Hongxi, Chen Shilin (2010): Identification of medicinal plants in the family Fabaceae using a potential DNA barcode ITS2. Journal of Ethnopharmacology, 130, 116-121
Genta Susana B., Cabrera Wilfredo M., Mercado María I., Grau Alfredo, Catalán César A., Sánchez Sara S. (2010): Hypoglycemic activity of leaf organic extracts from Smallanthus sonchifolius: Constituents of the most active fractions. Chemico-Biological Interactions, 185, 143-152
Hajslova Jana, Cajka Tomas, Vaclavik Lukas (2011): Challenging applications offered by direct analysis in real time (DART) in food-quality and safety analysis. TrAC Trends in Analytical Chemistry, 30, 204-218
Huang Min-Zong, Cheng Sy-Chi, Cho Yi-Tzu, Shiea Jentaie (2011): Ambient ionization mass spectrometry: A tutorial. Analytica Chimica Acta, 702, 1-15
Jorgensen J. L., Stehlik I., Brochmann C., Conti E. (2003): Implications of ITS sequences and RAPD markers for the taxonomy and biogeography of the Oxytropis campestris and O. arctica (Fabaceae) complexes in Alaska. American Journal of Botany, 90, 1470-1480
Lachman J., Fernández E.C., Orsák M. (2003): Yacon [Smallanthus sonchifolia (Poepp. et Endl.) H. Robinson] chemical composition and use – a review. Plant Soil Environment, 49: 283–290.
Lin Tzu Che, Yeh Mau Shing, Cheng Ya Ming, Lin Li Chang, Sung Jih Min (2012): Using ITS2 PCR-RFLP to generate molecular markers for authentication of Sophora flavescens Ait.. Journal of the Science of Food and Agriculture, 92, 892-898
Liu Zhihua, Zeng Xu, Yang Dan, Ren Guomin, Chu Guiyan, Yuan Zhengrong, Luo Kun, Xiao Peigen, Chen Shilin (2012): Identification of medicinal vines by ITS2 using complementary discrimination methods. Journal of Ethnopharmacology, 141, 242-249
Mangoni Olga, Imperatore Concetta, Tomas Carmelo, Costantino Valeria, Saggiomo Vincenzo, Mangoni Alfonso (2011): The New Carotenoid Pigment Moraxanthin Is Associated with Toxic Microalgae. Marine Drugs, 9, 242-255
Ovesná J., Kučera L., Vaculová K., Milotová J., Snape J., Wenzl P., Huttner E., Kilian A., Martelli G., Milella L. (2013): Analysis of the Genetic Structure of a Barley Collection Using DNA Diversity Array Technology (DArT). Plant Molecular Biology Reporter, 31, 280-288
Plchová R. (1997): Polymnia sonchifolia (Poepp. et Endl.) and its growing in Czech Republic. Agricultura Tropica et Subtropica, 30: 61–69.
Prchalová Jana, Kovařík František, Ševčík Rudolf, Čížková Helena, Rajchl Aleš (2014): Characterization of mustard seeds and paste by DART ionization with time-of-flight mass spectrometry. Journal of Mass Spectrometry, 49, 811-818
Rajchl Aleš, Drgová Ladislava, Grégrová Adéla, Čížková Helena, Ševčík Rudolf, Voldřich Michal (2013): Rapid determination of 5-hydroxymethylfurfural by DART ionization with time-of-flight mass spectrometry. Analytical and Bioanalytical Chemistry, 405, 4737-4745
Shen Yao, van Beek Teris A., Claassen Frank W., Zuilhof Han, Chen Bo, Nielen Michel W.F. (2012): Rapid control of Chinese star anise fruits and teas for neurotoxic anisatin by Direct Analysis in Real Time high resolution mass spectrometry. Journal of Chromatography A, 1259, 179-186
Slanc P., Ravnikar M., Strukelj B. (2006): Identification of individual herbal drugs in tea mixtures using restriction analysis of ITS DNA and real-time PCR. Pharmazie, 61: 912–915.
Sun Yung Wei, Liao Yu Ju, Hung Yin Sui, Chang Jia Ci, Sung Jih Min (2011): Development of ITS sequence based SCAR markers for discrimination of Paphiopedilum armeniacum, Paphiopedilum micranthum, Paphiopedilum delenatii and their hybrids. Scientia Horticulturae, 127, 405-410
Vaclavik Lukas, Cajka Tomas, Hrbek Vojtech, Hajslova Jana (2009): Ambient mass spectrometry employing direct analysis in real time (DART) ion source for olive oil quality and authenticity assessment. Analytica Chimica Acta, 645, 56-63
Vilhena Stela Maria C, Câmara Francisco Luiz A, Kakihara Sergio T (2000): O cultivo de yacon no Brasil. Horticultura Brasileira, 18, 5-8
White T.J., Bruns T.S., Lee S., Taylor J. (1990): Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenies, In: Innis M.A., Gelfand D.H., Sninsky J.J., White T.J. (eds.). PCR protocols. USA, Academic Press: 315–322.
Ziarovská Jana, Fernández Eloy, Millela Luigi (2013): A revised its nucleotide sequence gives a specifity for Smallanthus sonchifolius (Poepp. and Endl.) and its products identification. , 45, 217-226
Ziarovska Jana, Cusimamani Eloy, Milella Daniela (2014): Difference on ITS regions among Yacon genotypes and Smallanthus spp.. Emirates Journal of Food and Agriculture, 26, 60-
download PDF

© 2020 Czech Academy of Agricultural Sciences