Application of InDel markers based on the chloroplast genome sequences for authentication and traceability of tartary and common buckwheat

https://doi.org/10.17221/116/2016-CJFSCitation:Cho K., Hong S., Yun B., Won H., Yoon Y., Kwon K., Mekapogu M. (2017): Application of InDel markers based on the chloroplast genome sequences for authentication and traceability of tartary and common buckwheat. Czech J. Food Sci., 35: 122-130.
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A reliable, qualitative PCR-based detection method for the traceability and authentication of common and Tartary buckwheat was developed. Five InDel markers developed from chloroplast genome variation between the two species were applied for 96 buckwheat accessions and all accessions were easily differentiated as Tartary and common buckwheat using these markers. We also determined the sample detection limit by PCR and qPCR as 0.001 and 0.02 ng/µl, respectively. InDel markers could detect the mixture of two species flour up to 10% contamination. InDel markers were also applied to processed foods such as noodles and tea, and we found that species-specific PCR bands could be used to identify buckwheat even after processing. Hence, these InDel markers are simple with higher specificity and sensitivity and are reliable for the authentication of buckwheat processed foods.
References:
Abeywardena M.Y., Head R.J. (2001): Dietary polyunsaturated fatty acid and antioxidant modulation of vascular dysfunction in the spontaneously hypertensive rat. Prostaglandins, Leukotrienes and Essential Fatty Acids, 65, 91-97 https://doi.org/10.1054/plef.2001.0294
 
Barcaccia G., Volpato M., Gentili R., Abeli T., Galla G., Orsenigo S., Citterio S., Sgorbati S., Rossi G. (2016): Genetic identity of common buckwheat (Fagopyrum esculentum Moench) landraces locally cultivated in the Alps. Genetic Resources and Crop Evolution, 63, 639-651 https://doi.org/10.1007/s10722-015-0273-z
 
Bisht V., Singh B., Rao K.S., Maikhuri R.K., Nautiyal A.R. (2007): Genetic divergence of paddy landraces in Nanakosi micro-watershed of Uttarakhand Himalaya. Journal of Tropical Agriculture, 45: 48–50.
 
Bonafaccia G, Marocchini M, Kreft I (2003): Composition and technological properties of the flour and bran from common and tartary buckwheat. Food Chemistry, 80, 9-15 https://doi.org/10.1016/S0308-8146(02)00228-5
 
Chauhan R.S., Gupta N., Sharma S.K., Rana J.C., Sharma T.R., Jana S. (2010): Genetic and genome resources in buckwheat – present status and future perspectives. European Journal of Plant Science and Biotechnology, 4: 33–44.
 
Cho Kwang-Soo, Yun Bong-Kyoung, Yoon Young-Ho, Hong Su-Young, Mekapogu Manjulatha, Kim Kyung-Hee, Yang Tae-Jin, Heinze Berthold (2015): Complete Chloroplast Genome Sequence of Tartary Buckwheat (Fagopyrum tataricum) and Comparative Analysis with Common Buckwheat (F. esculentum). PLOS ONE, 10, e0125332- https://doi.org/10.1371/journal.pone.0125332
 
Fabjan Nina, Rode Janko, Košir Iztok Jože, Wang Zhuanhua, Zhang Zheng, Kreft Ivan (2003): Tartary Buckwheat ( Fagopyrum tataricum Gaertn.) as a Source of Dietary Rutin and Quercitrin. Journal of Agricultural and Food Chemistry, 51, 6452-6455 https://doi.org/10.1021/jf034543e
 
Futo S. (2002): PCR testing for food allergens in food products. Shokuhin Eiseigaku Zasshi, 43: J280–J282. (in Japaneese)
 
Guo Xiaona, Zhu Kexue, Zhang Hui, Yao Huiyuan (2010): Anti-Tumor Activity of a Novel Protein Obtained from Tartary Buckwheat. International Journal of Molecular Sciences, 11, 5201-5211 https://doi.org/10.3390/ijms11125201
 
HIRAO Takashi, IMAI Shinsuke, SAWADA Hiroshi, SHIOMI Nobuo, HACHIMURA Satoshi, KATO Hisanori (): PCR Method for Detecting Trace Amounts of Buckwheat ( Fagopyrum spp.) in Food. Bioscience, Biotechnology and Biochemistry, 69, 724-731 https://doi.org/10.1271/bbb.69.724
 
Howes N. K., Chong J., Brown P. D. (1992): Oat endosperm proteins associated with resistance to stem rust of oats. Genome, 35, 120-125 https://doi.org/10.1139/g92-020
 
Ikeda K. (2002): Buckwheat composition, chemistry, and processing. Advances in Food and Nutrition Research, 44: 395–434.
 
Ikeda S., Yamashita Y. (1994): Buckwheat as a dietary source of zinc, copper and manganese. Fagopyrum, 14: 29–34.
 
Janeš Damjan, Prosen Helena, Kreft Samo (2012): Identification and Quantification of Aroma Compounds of Tartary Buckwheat (Fagopyrum tataricum Gaertn.) and Some of Its Milling Fractions. Journal of Food Science, 77, C746-C751 https://doi.org/10.1111/j.1750-3841.2012.02778.x
 
Jeon Y.J., Kang E.S., Hong K.W. (2007): A PCR method for rapid detection of buckwheat ingredients in food. Journal of the Korean Society for Applied Biological Chemistry, 50: 276–280.
 
Kim J.K. Kim S.K. (2004): Physiochemical properties of buckwheat starches from different areas. Korean Journal of Food Science and Technology, 36: 598–603.
 
Kreft I. (1994): Traditional buckwheat food in Europe. Bulletin of the Research Institute for Food Science, 57: 1–8.
 
Kump Bojka, Javornik Branka (1996): Evaluation of genetic variability among common buckwheat (Fagopyrum esculentum Moench) populations by RAPD markers. Plant Science, 114, 149-158 https://doi.org/10.1016/0168-9452(95)04321-7
 
Li Si-quan, Zhang Q. Howard (2001): Advances in the Development of Functional Foods from Buckwheat. Critical Reviews in Food Science and Nutrition, 41, 451-464 https://doi.org/10.1080/20014091091887
 
Liu Z., Ishikawa W., Huang X., Tomotake H., Watanabe H., Kato N. (2001): Buckwheat protein product suppresses 1,2-dimethylohydrazine-induced colon carcinogenesis in rats by reducing cell proliferation. Nutrition and Cancer Research Communication, 131: 1850–1853.
 
Luthar Z. (1992): Polyphenol classification and tannin content of buckwheat seeds (Fagopyrum esculentum Moench). Fagopyrum, 12: 36–42.
 
Nagai Takeshi, Sakai Mizuho, Inoue Reiji, Inoue Hachiro, Suzuki Nobutaka (2001): Antioxidative activities of some commercially honeys, royal jelly, and propolis. Food Chemistry, 75, 237-240 https://doi.org/10.1016/S0308-8146(01)00193-5
 
Nair Arun, Adachi Taiji (2002): Screening and Selection of Hypoallergenic Buckwheat Species. The Scientific World JOURNAL, 2, 818-826 https://doi.org/10.1100/tsw.2002.157
 
Ohnishi Ohmi, Matsuoka Yoshihiro (1996): Search for the wild ancestor of buckwheat II. Taxonomy of Fagopyrum (Polygonaceae) species based on morphology, isozymes and cpDNA variability.. Genes & Genetic Systems, 71, 383-390 https://doi.org/10.1266/ggs.71.383
 
Ohsako Takanori, Yamane Kyoko, Ohnishi Ohmi (): Two new Fagopyrum (Polygonaceae) species, F. gracilipedoides and F. jinshaense from Yunnan, China. Genes & Genetic Systems, 77, 399-408 https://doi.org/10.1266/ggs.77.399
 
Pacurar D. I., Pacurar M. L., Street N., Bussell J. D., Pop T. I., Gutierrez L., Bellini C. (): A collection of INDEL markers for map-based cloning in seven Arabidopsis accessions. Journal of Experimental Botany, 63, 2491-2501 https://doi.org/10.1093/jxb/err422
 
Paradkar M.M, Irudayaraj J (2002): Discrimination and classification of beet and cane inverts in honey by FT-Raman spectroscopy. Food Chemistry, 76, 231-239 https://doi.org/10.1016/S0308-8146(01)00292-8
 
Park C.H., Kim Y.B., Choi Y.S., Heo K., Kim S.L., Lee K.C., Chang K.J., Lee H.B. (2000): Rutin content in food products processed from groats, leaves and flowers of buckwheat. Fagopyrum, 17: 63–66.
 
Qin Peiyou, Ma Tingjun, Wu Li, Shan Fang, Ren Guixing (2011): Identification of Tartary Buckwheat Tea Aroma Compounds with Gas Chromatography-Mass Spectrometry. Journal of Food Science, 76, S401-S407 https://doi.org/10.1111/j.1750-3841.2011.02223.x
 
Wojcicki J., Barcewwiszniewska B., Samochowiec L., Rozewicka L. (1995): Extractum-Fagopyri reduces atherosclerosis in high-fat diet fed rabbits. Pharmazie, 50: 560–562.
 
YAMAKAWA Hirohito, AKIYAMA Hiroshi, ENDO Yumi, MIYATAKE Kiyoko, SAKAI Shinobu, KONDO Kazunari, TOYODA Masatake, URISU Atsuo, TESHIMA Reiko (): Specific Detection of Buckwheat Residues in Processed Foods by Polymerase Chain Reaction. Bioscience, Biotechnology and Biochemistry, 72, 2228-2231 https://doi.org/10.1271/bbb.80237
 
Yamaki Shinichiro, Ohyanagi Hajime, Yamasaki Masanori, Eiguchi Mitsugu, Miyabayashi Toshie, Kubo Takahiko, Kurata Nori, Nonomura Ken-Ichi (2013): Development of INDEL markers to discriminate all genome types rapidly in the genus Oryza. Breeding Science, 63, 246-254 https://doi.org/10.1270/jsbbs.63.246
 
Yoon Jae-Wook, Jung Jae-Yeon, Chung Hyun-Jung, Kim Mi-Ryung, Kim Chan-Wha, Lim Seung-Taik (2010): Identification of botanical origin of starches by SDS-PAGE analysis of starch granule-associated proteins. Journal of Cereal Science, 52, 321-326 https://doi.org/10.1016/j.jcs.2010.06.015
 
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