The activities of amylases and α-amylase inhibitor in wide-range herbicide resistant wheat lines
M. Nagy-Gasztonyi, A. Nagy, E. Németh-SZerdahelyi, J. Pauk, É. Gelencsérhttps://doi.org/10.17221/125/2009-CJFSCitation:Nagy-Gasztonyi M., Nagy A., Németh-SZerdahelyi E., Pauk J., Gelencsér É. (2010): The activities of amylases and α-amylase inhibitor in wide-range herbicide resistant wheat lines. Czech J. Food Sci., 28: 217-224.
The activities of total amylase, α-amylase, and α-amylase inhibitor in the albumin-globulin fractions of isogenic non transgenic control (CY 45) and ppt (phosphinothrichin) resistant transgenic spring wheat (Triticum aestivum L.) lines (T106, T116, T117, T124, T128, T129) were studied in two subsequent years. The plants were either sprayed with a selective herbicide Granstar (G), a wide range herbicide Finale 14 SL (F), or were grown without spraying (Q). Samples were obtained from field trial experiments of the Cereal Research Non Profit Co (Szeged, Hungary). Our results showed an increased trend in total amylase activity of untreated transgenic wheat lines in comparison with non transgenic wheat. The herbicide treatments enhanced the total amylase activity in both transgenic and non transgenic wheat samples. The changes in α-amylase inhibitor activity showed the same trend as that observed in total amylase activity in transgenic lines.Keywords:
transgenic wheat; amylolytic activities; α-amylase inhibitor; electrophoresis; specific staining