Molecular cloning and characterisation of alpha subunit of H+-ATPase in Lactobacillus casei Zhang

https://doi.org/10.17221/134/2008-CJFSCitation:Chen X., Yang M., Sun Z., Liu W., Sun T., Meng H., Zhang H. (2009): Molecular cloning and characterisation of alpha subunit of H+-ATPase in Lactobacillus casei Zhang. Czech J. Food Sci., 27: 49-54.
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The acid tolerance is an important property of Lactic acid bacteria as potential probiotics. H+-ATPase is considered a key gene in several bacteria with the ability of acid tolerance. We cloned and sequenced the full length cDNA of alpha subunit of H+-ATPase gene in Lactobacillus casei Zhang, which had been isolated from traditional home-made koumiss in Inner Mongolia of China. The results showed that the respective cDNA sequence is composed of 1530 nucleotides and codes a putative protein including 509 amino acids. In addition, we also reconstructed the phylogenic trees for H+-ATPase gene based on amino acids sequences of diverse strains of Lactic acid bacteria.
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