Molecular cloning and characterisation of alpha subunit of H+-ATPase in Lactobacillus casei Zhang
X. Chen, M. Yang, Z. Sun, W. Liu, T. Sun, H. Meng, H. Zhanghttps://doi.org/10.17221/134/2008-CJFSCitation:Chen X., Yang M., Sun Z., Liu W., Sun T., Meng H., Zhang H. (2009): Molecular cloning and characterisation of alpha subunit of H+-ATPase in Lactobacillus casei Zhang. Czech J. Food Sci., 27: 49-54.
The acid tolerance is an important property of Lactic acid bacteria as potential probiotics. H+-ATPase is considered a key gene in several bacteria with the ability of acid tolerance. We cloned and sequenced the full length cDNA of alpha subunit of H+-ATPase gene in Lactobacillus casei Zhang, which had been isolated from traditional home-made koumiss in Inner Mongolia of China. The results showed that the respective cDNA sequence is composed of 1530 nucleotides and codes a putative protein including 509 amino acids. In addition, we also reconstructed the phylogenic trees for H+-ATPase gene based on amino acids sequences of diverse strains of Lactic acid bacteria.Keywords:H+-ATPase; clone; sequence; Lactobacillus casei Zhang; phylogenic trees