Rapid detection of dimethyl yellow dye in curry by liquid chromatography-electrospray-tandem mass spectrometry
F. Tateo, M. Bononi, F. Gallonehttps://doi.org/10.17221/135/2009-CJFSCitation:Tateo F., Bononi M., Gallone F. (2010): Rapid detection of dimethyl yellow dye in curry by liquid chromatography-electrospray-tandem mass spectrometry. Czech J. Food Sci., 28: 427-432.
An accurate and rapid method, was devised for the identification and quantitation of dimethyl yellow dye in curry, based on liquid chromatography-tandem mass spectrometry interfaced with electrospray. Mass spectral acquisition was done in positive ion mode applying two fragmentation transitions to provide a high degree of selectivity. The extraction system provided a very high recovery (100.0% to 105.8%) and good results were obtained for the limit of detection (5 μg/kg) and limit of quantitation (16 μg/kg). The applicability of the method to identifing and quantifing the unauthorised dimethyl yellow dye in curry was demonstrated.Keywords:
azo dyes; dimethyl yellow; curry; LC-ESI-/MS/MS