FT-IR spectroscopic characteristics of differently cultivated Escherichia coli
Z. Filip, S. Hermann, K. Demnerováhttps://doi.org/10.17221/14/2008-CJFSCitation:Filip Z., Hermann S., Demnerová K. (2008): FT-IR spectroscopic characteristics of differently cultivated Escherichia coli. Czech J. Food Sci., 26: 458-463.
FT-IR spectra were recorded of Escherichia coli cell mass with the aim of obtaining spectral traits possibly useful in a rapid detection and characterisation of this indicator bacterium. A well differentiated spectrum was obtained from the cell mass harvested in a stationary phase of growth, e.g., after 24 h, from a minimum nutrient broth. The cell mass, harvested either earlier or grown in nutrient solutions which contained an enhanced carbon or nitrogen concentrations delivered somewhat different IR spectra, apparently due to a higher content of nucleic acid components as related to other structural constituents of bacterial cells. Consequently, the FT-IR spectra of E. coli, although rather rapidly to collect, seem only capable of delivering useful and reproducible information if the cell mass is obtained under standardised cultural conditions.Keywords:Escherichia coli; FT-IR spectroscopy; cell mass traits