Influence of nitrogen sources on growth of thraustochytrids in waste water from the demineralization of cheese whey T., Kronusová O., Kaštánek P., Potočár T., Kohoutková J., Brányik T. (2019): Influence of nitrogen sources on growth of thraustochytrids in waste water from the demineralization of cheese whey. Czech J. Food Sci., 37: 383-390.
download PDF

An experimental design was ued to optimize the growth of two thraustochytrids, (Schizochytrium limacinum PA-968 and Japonochytrium marinum AN-4), on different nitrogen sources (yeast extract, corn steep liquor, ammonium sulphate) supplemented into saline waste water from the demineralization of cheese whey. Yeast extract was found to be the most suitable complex nutrient source. Nitrogen limitation was found to increase the lipid content in shake flask cultures of thraustochytrids by 12.7–22.4% w/w. The maximum total lipid content (79.1% w/w) and docosahexaenoic acid productivity (0.465 g/l per day) were achieved by J. marinum AN-4 in shake flask cultures. Fed-batch cultures of J. marinum AN-4, under conditions of nitrogen limitation, yielded biomass with a lower lipid content (72.1% wt.) but higher docosahexaenoic acid productivity (1.43 g/l per day). These results provide proof of concept that fed-batch cultivation of thraustochytrids, combined with nitrogen limitation, can be an appropriate strategy for the productive use of saline waste water from the dairy industry.

Aasen I.M., Ertesvag H., Heggeset T.M., Liu B., Brautaset T., Vadstein O., Ellingsen T.E. (2016): Thraustochytrids as production organisms for docosahexaenoic acid (DHA), squalene, and carotenoids. Applied Microbiology and Biotechnology, 100: 4309–21.
Abdus-Salaam R.B., Adepoju P.A., Olaleye O.N., Adeoye I.A. (2014): Studies on the antimicrobial effect of corn steep liquor on some diarrhoea causing organisms. African Journal of Biotechnology, 13: 332–335.
Chang G., Gao N., Tian G., Wu Q., Chang M., Wang X. (2013): Improvement of docosahexaenoic acid production on glycerol by Schizochytrium sp. S31 with constantly high oxygen transfer coefficient. Bioresource Technology, 142: 400–406.
Chi Z., Pyle D., Wen Z., Frear C., Chen S. (2007): A laboratory study of producing docosahexaenoic acid from biodiesel-waste glycerol by microalgal fermentation. Process Biochemistry. 42: 1537–1545.
Chi Z., Liu Y., Frear C., Chen S. (2009): Study of a two-stage growth of DHA-producing marine algae Schizochytrium limacinum SR21 with shifting dissolved oxygen level. Applied Microbiology and Biotechnology, 81: 1141–1148.
Chinta Y.D., Kano K., Widiastuti A., Fukahori M., Kawasaki S., Eguchi Y., Misu H., Odani H., Zhou S., Narisawa K., Fujiwara K., Shinoharae M., Sato T. (2014): Effect of corn steep liquor on lettuce root rot (Fusarium oxysporum f. sp. lactucae) in hydroponic cultures. Journal of the Science of Food and Agricilture, 94: 2317–2323.
Diblíková L., Čurda L., Kinčl J. (2013): The effect of dry matter and salt addition on cheese whey demineralisation. International Dairy Journal, 31: 29–33.
Ethier S., Woisard K., Vaughan D., Wen Z. (2011): Continuous culture of the microalgae Schizochytrium limacinum on biodiesel-derived crude glycerol for producing docosahexaenoic acid. Bioresource Technology, 102: 88–93.
Ganuza E., Izquierdo M.S. (2007): Lipid accumulation in Schizochytrium G13/2S produced in continuous culture. Applied Microbiology and Biotechnology, 76: 985–990.
Gupta A., Barrow C.J., Puri M. (2012): Omega-3 biotechnology: Thraustochytrids as a novel source of omega-3 oils. Biotechnology Advances, 30: 1733–1745.
Honda D., Yokochi T., Nakahara T., Erata M., Higashihara T. (1998): Schizochytrium limacinum sp. nov., a new thraustochytrid from a mangrove area in the west Pacific Ocean. Mycological Research, 102: 439–448.
Humhal T., Kastanek P., Jezkova Z., Cadkova A., Kohoutkova J., Branyik T. (2017): Use of saline waste water from demineralization of cheese whey for cultivation of Schizochytrium limacinum PA-968 and Japonochytrium marinum AN-4. Bioprocess and Biosystems Engineering, 40: 395–402.
Jakobsen A.N., Aasen I.M., Josefsen K.D., Strom A.R. (2008): Accumulation of docosahexaenoic acid-rich lipid in thraustochytrid Aurantiochytrium sp. strain T66: effects of N and P starvation and O2 limitation. Applied Microbiology and Biotechnology, 80: 297–306.
Jiang X., Zhang J., Zhao J., Gao Z., Zhang C., Chen M. (2017): Regulation of lipid accumulation in Schizochytrium sp. ATCC 20888 in response to different nitrogen sources. European Journal of Lipid Science and Technology, 119: 1700025.
Li J., Liu R., Chang G., Li X., Chang M., Liu Y., Jin Q., Wang X. (2015): A strategy for the highly efficient production of docosahexaenoic acid by Aurantiochytrium limacinum SR21 using glucose and glycerol as the mixed carbon sources. Bioresource Technology, 177: 51–57.
Ling X., Guo J., Liu X., Zhang X., Wang N., Lu Y., Ng I.S. (2015): Impact of carbon and nitrogen feeding strategy on high production of biomass and docosahexaenoic acid (DHA) by Schizochytrium sp. LU310. Bioresource Technology, 184: 139–147.
Luy M., Rusing M. (2014): Process for cultivating microorganisms of the genus Thraustochytriales. United States Patent 8889382B2.
Pyle D.J., Garcia R.A., Wen Z. (2008): Producing docosahexaenoic acid (DHA)-rich algae from biodiesel-derived crude glycerol: effects of impurities on DHA production and algal biomass composition. Journal of Agricultural and Food Chemistry, 56: 3933–3939.
Qu L., Ren, L.J., Huang, H. (2013): Scale-up of docosahexaenoic acid production in fed-batch fermentation by Schizochytrium sp. based on volumetric oxygen-transfer coefficient. Biochemical Engineering Journal, 77: 82–87.
Procházková G., Brányiková I., Zachleder V., Brányik T. (2014): Effect of nutrient supply status on biomass composition of eukaryotic green microalgae. Journal of Applied Phycology, 26:1359–1377.
Ratledge C. (2004): Fatty acid biosynthesis in microorganisms being used for single cell oil production. Biochimie, 86: 807–815.
Raghukumar S. (2008): Thraustochytrid marine protists: production of PUFAs and other emerging technologies. Marine Biotechnology, 10: 631–40.
Tsui C.K., Marshall W., Yokoyama R., Honda D., Lippmeier J.C., Craven K.D., Peterson P. D., Berbee M.L. (2009): Labyrinthulomycetes phylogeny and its implications for the evolutionary loss of chloroplasts and gain of ectoplasmic gliding. Molecular Phylogenetics and Evolution, 50: 129–40.
Wu S.T., Yu S.T., Lin L.P. (2005): Effect of culture conditions on docosahexaenoic acid production by Schizochytrium sp. S31. Process Biochemistry, 40: 3103–3108.
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti