Purification, characterization and in vitro bile salt-binding capacity of polysaccharides from Armillaria mellea mushroom

https://doi.org/10.17221/182/2018-CJFSCitation:Yue C., Zang X., Chen C., Dong L., Liu Y., Yu G. (2019): Purification, characterization and in vitro bile salt-binding capacity of polysaccharides from Armillaria mellea mushroom. Czech J. Food Sci., 37: 51-56.
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The crude polysaccharides from Armillaria mellea were obtained with an ultrasound assisted enzymatic extraction and ethanol precipitation. Two polysaccharide fractions were obtained by ethanol precipitation, which were named AMP-1 and AMP-2. The results of the monosaccharide composition analysis indicated that AMP-1 was composed of mannose, rhamnose, glucose, galactose, arabinose and fucose and that AMP-2 was composed of mannose, rhamnose, glucose, galactose and fucose. Glucose and galactose were the main monosaccharide fractions. The protein and nucleic acid contents in AMP-1 and AMP-2 were detected by using ultraviolet and infrared spectroscopy. The bile salt-binding capacities of the polysaccharide samples were studied in vitro. In comparison with lentinan (LP), AMP-1 and AMP-2 showed increased bile salt-binding capacity. AMP-1 showed the highest binding capacity against all the bile salts. The findings presented in this study highlight the potential of the A. mellea polysaccharides as a natural hypolipidaemic agent.

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