Monitoring of imidazole dipeptides in meat products by capillary zone electrophoresis

https://doi.org/10.17221/192/2019-CJFSCitation:Szerdahelyi E., Csehi B., Takács K., Korompai E., Nagy A., Gelencsér É., Friedrich L.F. (2020): Monitoring of imidazole dipeptides in meat products by capillary zone electrophoresis. Czech J. Food Sci., 38: 36-42.
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Abstract: A simple capillary zone electrophoretic technique (CZE) was developed for the determination of carnosine and anserine, and the main analytical performance characteristics were determined. The method was used for an analysis of raw meat samples, heat treated as well as high hydrostatic pressure (HHP) treated meat samples, and various meat products. The effect of heat treatment (10 min at 75 °C and 45 min at 90 °C) and HHP (100–600 MPa, 5 min) was investigated on pork longissimus thoracis muscle samples. With the exception of the milder heat treatment a slight decrease was detected in dipeptide contents of treated samples, but significant differences (P < 0.05) were not observed under any treatment. Thirty-two meat-based food products were also analysed. Imidazole dipeptides were detectable in all of them. The poultry products showed a characteristically low carnosine/anserine ratio. The data obtained were consistent with the food label information.

References:
Abe H., Okuma E. (1995): Discrimination of meat species in processed meat products based on the ratio of histidine dipeptides. Journal of the Japanese Society for Food Science & Technology, 42: 827–834.
 
Aristoy M.C., Toldrá F. (2004a): A simple, fast and reliable methodology for the analysis of histidine dipeptides as markers of presence of animal origin proteins in feeds for ruminants. Food Chemistry, 84: 485–491. https://doi.org/10.1016/j.foodchem.2003.07.030
 
Aristoy M.C., Toldrá F. (2004b): Histidine dipeptides HPLC-based test for the detection of mammalian origin proteins in feeds for ruminants. Meat Science, 67: 211–217. https://doi.org/10.1016/j.meatsci.2003.10.008
 
D’Astous-Pagé J., Gariépy C., Blouin R., Cliche S., Méthot S., Sullivan B., Fortin F., Palin M.F. (2017): Carnosine content in the porcine longissimus thoracis muscle and its association with meat quality attributes and carnosine-related gene expression. Meat Science, 124: 84–94. https://doi.org/10.1016/j.meatsci.2016.11.004
 
de Castro R.J.S., Sato H.H. (2015): Biologically active peptides: Processes for their generation, purification and identification and applications as natural additives in the food and pharmaceutical industries. Food Research International, 74: 185–198. https://doi.org/10.1016/j.foodres.2015.05.013
 
Dragsted L.O. (2010): Biomarkers of meat intake and the application of nutrigenomics. Meat Science, 84: 301–307. https://doi.org/10.1016/j.meatsci.2009.08.028
 
Gariballa S.E., Sinclair A.J. (2000): Carnosine: physiological properties and therapeutic potential. Age and Ageing, 29: 207–210. https://doi.org/10.1093/ageing/29.3.207
 
Gulewitch W.S., Amiradzibi S. (1900): Über das Carnosin, eine neue organische Base des Fleischextraktes. (About carnosine, a new organic base of the meat extract.) Berichte der Deutschen Chemischen Gesellschaft (The German Chemical Society Reports), 33: 1902–1904. https://doi.org/10.1002/cber.19000330275
 
Harris R.C., Wise J.A., Price K.A., Kim H.J., Kim C.K., Sale C. (2012): Determinants of muscle carnosine content. Amino Acids, 43: 5–12. https://doi.org/10.1007/s00726-012-1233-y
 
Hermanussen M., Gonder U., Jakobs C., Stegemann D., Hoffmann G. (2010): Patterns of free amino acids in German convinience food products: marked mismatch between label information and composition. European Journal of Clinical Nutrition, 64: 88–98. https://doi.org/10.1038/ejcn.2009.116
 
Huang Y., Duan J., Chen H., Chen M., Chen G. (2005): Separation and determination of carnosine-related peptides using capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis, 26: 593–599. https://doi.org/10.1002/elps.200406130
 
Hugas M., Garriga M., Monfort J.M. (2002): New mild technologies in meat processing: high pressure as a model technology. Meat Science, 62: 359–371. https://doi.org/10.1016/S0309-1740(02)00122-5
 
Jayasena D.D., Jung S., Bae Y.S., Park H.B., Lee J.H., Jo C. (2015): Comparison of the amounts of endogenous bioactive compounds in raw and cooked meats from commercial broilers and indigenous chickens. Journal of Food Composition and Analysis, 37: 20–24. https://doi.org/10.1016/j.jfca.2014.06.016
 
Jiru M., Stranska-Zachariasova M., Kocourek V., Krmela A., Tomaniova M., Jan Rosmus J., Hajslova J. (2019): Authentication of meat species and net muscle proteins: updating of an old concept. Czech Journal of Food Sciences, 37: 205–211. https://doi.org/10.17221/94/2019-CJFS
 
Jozanović M., Hajduković M., Galović O., Kralik G., Kralik Z., Sakač N., Medvidović-Kosanović M., Sak-Bosnar M. (2017): Determination of anti-oxidative histidine dipeptides in poultry by microchip capillary electrophoresis with contactless conductivity detection. Food Chemistry, 221: 1658–1665. https://doi.org/10.1016/j.foodchem.2016.10.122
 
Kantha S. S., Takeuchi M., Watabe S., Ochi H. (2000): HPLC determination of carnosine in commercial canned soups and natural meat extracts. LWT – Food Science and Technology, 33: 60–62. https://doi.org/10.1006/fstl.1999.0602
 
Ma X.Y., Jiang Z.Y., Lin Y.C., Zheng C.T., Zhou G.L. (2010): Dietary supplementation with carnosine improves antioxidant capacity and meat quality of finishing pigs. Journal of Animal Physiology and Animal Nutrition, 94: 286–295. https://doi.org/10.1111/j.1439-0396.2010.01009.x
 
Maikhunthod B., Intarapichet K.O. (2005): Heat and ultrafiltration extraction of broiler meat carnosine and its antioxidant activity. Meat Science, 71: 364–374. https://doi.org/10.1016/j.meatsci.2005.04.017
 
Mora M., Sentandreu M. Á., Toldrá F. (2007): Hydrophilic chromatographic determination of carnosine, anserine, balenine, creatine, and creatinine. Journal of Agricultural and Food Chemistry, 55: 4664–4669. https://doi.org/10.1021/jf0703809
 
Mori M., Mizuno D., Konaka-Mizuno K., Sadakane Y., Kawahara M. (2015): Quantitative analysis of carnosine and anserine in foods by performing high performance liquid chromatography. Biomedical Research on Trace Elements, 26: 147–152.
 
Park Y.J., Volpe S.L., Decker E.A. (2005): Quantitation of carnosine in humans plasma after dietary consumption of beef. Journal of Agricultural and Food Chemistry, 15: 4736–4739. https://doi.org/10.1021/jf047934h
 
Staňová A., Marák J., Rezeli M., Páger Cs. Kilár. F. Kaniansky D. (2011): Analysis of therapeutic peptides in human urine by combination of capillary zone electrophoresis –electrospray mass spectrometry with preparative capillary isotachophoresis sample pretreatment. Journal of Chromatography A, 48: 8701–8707. https://doi.org/10.1016/j.chroma.2011.09.080
 
Suzuki A., Homma N., Fukuda A., Hirao K., Uryu T. (1994): Effects of high pressure treatment on the flavour-related components in meat. Meat Science, 37: 369–379. https://doi.org/10.1016/0309-1740(94)90053-1
 
Tian Y., Xie M., Wang W., Wu H., Fu Z., Lin, L. (2007): Determination of carnosine in Black-Bone Silky Fowl (Gallus gallus domesticus Brisson) and common chicken by HPLC. European Food Research and Technology, 226: 311–314. https://doi.org/10.1007/s00217-006-0528-1
 
Tomonaga S., Hayakawa T., Yamane H., Maemura H., Sato M., Takahata Y., Morimatsu F., Furuse M. (2007): Oral administration of chicken breast extract increases brain carnosine and anserine concentrations in rats. Nutritional Neuroscience, 10: 181–186. https://doi.org/10.1080/10284150701587338
 
Zinellu A., Sotgia S., Campesi I., Franconi F., Deiana L., Carru C. (2011): Measurement of carnosine and anserine by FASI capillary electrophoresis UV detection: applications in biological samples. Talanta, 84: 931–935. https://doi.org/10.1016/j.talanta.2011.02.030
 
Zunic G., Spasic S. (2008): Capillary electrophoresis method optimized with factorial design for the determination of glutathione and amino acid status using human capillary blood. Journal of Chromatography B, 873: 70–76. https://doi.org/10.1016/j.jchromb.2008.07.036
 
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