Preparation, separation and antioxidant properties of hydrolysates derived from Grifola frondosa protein
G. Dong Y Qi, Z. Yang, H. Wang, S. Wang, G. Chenhttps://doi.org/10.17221/197/2015-CJFSCitation:Dong Y Qi G., Yang Z., Wang H., Wang S., Chen G. (2015): Preparation, separation and antioxidant properties of hydrolysates derived from Grifola frondosa protein. Czech J. Food Sci., 33: 500-506.
In order to take full advantage of Grifola frondosa resources, the protein (GFP) was extracted from G. frondosa fruiting body and digested using six different proteases (papain, neutrase, alcalase, trypsin, pepsin, and Protamex) to produce the antioxidative hydrolysates. The trypsin hydrolysate prepared at 1.5 h possessed the strongest antioxidant potential among different hydrolysates, which was separated into four major fractions by ultrafiltration membranes with different molecular weight cut-off (MWCO), that are GFHT-1 (Mw > 10 kDa), GFHT-2 (Mw = 5–10 kDa), GFHT-3 (Mw = 3–5 kDa), and GFHT-4 (Mw < 3 kDa). In succession, the in vitro antioxidant activities of the four fractions were further evaluated, including the scavenging effect on DPPH, ferrous ion chelating effect, reducing power, and ability to inhibit the autoxidation of linoleic acid. The results demonstrated that all fractions are effective antioxidants and comparably GFHT-4 has the highest antioxidant activity. Then GFHT-4 was separated to two major peptide fractions by gel filtration chromatography, and the two fractions were located at 2,385 Da (F-1) and 1,138 Da (F-2), respectively.Keywords:mushroom; trypsin antioxidant activity; peptidesReferences:
Hernández-Ledesma Blanca, Quirós Ana, Amigo Lourdes, Recio Isidra (2007): Identification of bioactive peptides after digestion of human milk and infant formula with pepsin and pancreatin. International Dairy Journal, 17, 42-49 https://doi.org/10.1016/j.idairyj.2005.12.012Chen Hua-Ming, Muramoto Koji, Yamauchi Fumio, Nokihara Kiyoshi (1996): Antioxidant Activity of Designed Peptides Based on the Antioxidative Peptide Isolated from Digests of a Soybean Protein. Journal of Agricultural and Food Chemistry, 44, 2619-2623 https://doi.org/10.1021/jf950833mChen Gui-tang, Zhao Lin, Zhao Li-yan, Cong Tao, Bao Shan-fen (2007): In vitro study on antioxidant activities of peanut protein hydrolysate. Journal of the Science of Food and Agriculture, 87, 357-362 https://doi.org/10.1002/jsfa.2744Chen G.T., Ma X.M., Liu S.T., Liao Y.L., Zhao G.Q. (2012a): Isolation, puriﬁcation and antioxidant activities of polysaccharides from Grifola frondosa. Carbohydrate Polymers, 89: 61–66.Chen N., Yang H., Sun Y., Niu J., Liu S. (2012b): Purification and identification of antioxidant peptides from walnut (Juglans regia L.) protein hydrolysates. Peptides, 38: 344–349.Chen G., Wang L., Yao S., Qi G., Liu X. (2014): Extraction technology of polysaccharide and protein from Grifola frondosa. Food Science and Technology, 39: 248–251. (in Chinese)Choe Eunok, Min David B. (2009): Mechanisms of Antioxidants in the Oxidation of Foods. Comprehensive Reviews in Food Science and Food Safety, 8, 345-358 https://doi.org/10.1111/j.1541-4337.2009.00085.xDinis T.C.P., Madeira V.M.C., Almeida L.M. (1994): Action of Phenolic Derivatives (Acetaminophen, Salicylate, and 5-Aminosalicylate) as Inhibitors of Membrane Lipid Peroxidation and as Peroxyl Radical Scavengers. Archives of Biochemistry and Biophysics, 315, 161-169 https://doi.org/10.1006/abbi.1994.1485Gibbs Bernard F., Zougman Alexandre, Masse Robert, Mulligan Catherine (2004): Production and characterization of bioactive peptides from soy hydrolysate and soy-fermented food. Food Research International, 37, 123-131 https://doi.org/10.1016/j.foodres.2003.09.010Kim Soo-Yong, Je Jae-Young, Kim Se-Kwon (2007): Purification and characterization of antioxidant peptide from hoki (Johnius belengerii) frame protein by gastrointestinal digestion. The Journal of Nutritional Biochemistry, 18, 31-38 https://doi.org/10.1016/j.jnutbio.2006.02.006Liu Chun, Wang Hongling, Cui Zhumei, He Xiaoling, Wang Xiansheng, Zeng Xiaoxiong, Ma Hao (2007): Optimization of extraction and isolation for 11S and 7S globulins of soybean seed storage protein. Food Chemistry, 102, 1310-1316 https://doi.org/10.1016/j.foodchem.2006.07.017Lund Marianne N., Heinonen Marina, Baron Caroline P., Estévez Mario (2011): Protein oxidation in muscle foods: A review. Molecular Nutrition & Food Research, 55, 83-95 https://doi.org/10.1002/mnfr.201000453Moure Andrés, Domínguez Herminia, Parajó Juan Carlos (2006): Antioxidant properties of ultrafiltration-recovered soy protein fractions from industrial effluents and their hydrolysates. Process Biochemistry, 41, 447-456 https://doi.org/10.1016/j.procbio.2005.07.014Nilsang Suthasinee, Lertsiri Sittiwat, Suphantharika Manop, Assavanig Apinya (2005): Optimization of enzymatic hydrolysis of fish soluble concentrate by commercial proteases. Journal of Food Engineering, 70, 571-578 https://doi.org/10.1016/j.jfoodeng.2004.10.011OSAWA Toshihiko, NAMIKI Mitsuo (1981): A novel type of antioxidant isolated from leaf wax of Eucalyptus leaves.. Agricultural and Biological Chemistry, 45, 735-739 https://doi.org/10.1271/bbb1961.45.735Oyaizu Makoto (1986): Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine.. The Japanese Journal of Nutrition and Dietetics, 44, 307-315 https://doi.org/10.5264/eiyogakuzashi.44.307Sami S., Deratani A., Belleville M.-P.., Amar B.B. (2014): Antioxidant properties of peptide fractions from tuna dark muscle protein by-product hydrolysate produced by membrane fractionation process. Food Research International, 65(C): 329–336.Sarmadi Bahareh H., Ismail Amin (2010): Antioxidative peptides from food proteins: A review. Peptides, 31, 1949-1956 https://doi.org/10.1016/j.peptides.2010.06.020Shimada Kazuko., Fujikawa Kuniko., Yahara Keiko., Nakamura Takashi. (1992): Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of Agricultural and Food Chemistry, 40, 945-948 https://doi.org/10.1021/jf00018a005Sun Qian, Shen Huixing, Luo Yongkang (2011): Antioxidant activity of hydrolysates and peptide fractions derived from porcine hemoglobin. Journal of Food Science and Technology, 48, 53-60 https://doi.org/10.1007/s13197-010-0115-0Wang X., HE L., Liu B. (2008): Study on extraction, purification and scavenging activity to hydroxyl radicals of polysaccharides from leaves of Ilex kudincha C. J. Tseng. Food Science, 29: 37–40.Wasser Solomon P. (2011): Current findings, future trends, and unsolved problems in studies of medicinal mushrooms. Applied Microbiology and Biotechnology, 89, 1323-1332 https://doi.org/10.1007/s00253-010-3067-4