Bee breads from two regions of Eastern Ukraine: composition, physical properties and biological activities R., Shevtsova T.S., Kružík V., Škorpilová T., Saloň I., Erban V., Brindza J., Brovarskyi V., Sinica A. (2019): Bee breads from two regions of Eastern Ukraine: composition, physical properties and biological activities. Czech J. Food Sci., 37: 9-20.
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Five samples of polyfloral bee bread originated from two regions of Eastern Ukraine were characterised by microscopic (SEM) and spectroscopic (FT MIR ATR, FT NIR, FT Raman and Vis) methods. Identification of pollens was based on the SEM images. Spectral differences were interpreted in terms of specific contribution of the main chemical constituents (proteins, phenolics, sugars, etc.) and pigments (flavonoids and carotenoids) of bee breads. PCA of FT NIR and Vis data was used for discrimination of bee bread samples according to botanical sources. The morphometric parameters, antioxidant capacity and prebiotic ef fects of bee breads were also studied. Obtained results expand the knowledge in the composition and properties of this beekeeping product.

Abouda Z., Zerdani I., Kalalou I., Faid M., Ahami M.T. (2011): The Antibacterial Activity of Moroccan Bee Bread and Bee-Pollen (Fresh and Dried) against Pathogenic Bacteria. Research Journal of Microbiology, 6, 376-384
Barth Andreas (2007): Infrared spectroscopy of proteins. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1767, 1073-1101
Bobiş O., Mărghitaş L.A., Dezmirean D., Morar O., Bonta V., Chirilă F. (2010): Quality parameters and nutritional value of different commercial bee products. Bulletin UASVM Animal Science and Biotechnologies, 67: 1–2.
Brovarskyi V., Velychko S., Brindza J., Adamchuk L. (2017): Development and testing of the technology of production of the beebread with the use of artificial combs. Agrobiodiversity for improving nutrition, health and life quality, 1: 31–42.
Campos Maria G. R., Bogdanov Stefan, de Almeida-Muradian Ligia Bicudo, Szczesna Teresa, Mancebo Yanina, Frigerio Christian, Ferreira Francisco (2015): Pollen composition and standardisation of analytical methods. Journal of Apicultural Research, 47, 154-161
CARPES Solange Teresinha (2009): Chemical composition and free radical scavenging activity of Apis mellifera bee pollen from Southern Brazil. BRAZILIAN JOURNAL OF FOOD TECHNOLOGY, 12, 220-229
De-Melo Adriane Alexandre Machado, Estevinho Maria Leticia Miranda Fernandes, Sattler José Augusto Gasparotto, Souza Bianca Rodrigues, Freitas Alex da Silva, Barth Ortrud Monika, Almeida-Muradian Ligia Bicudo (2016): Effect of processing conditions on characteristics of dehydrated bee-pollen and correlation between quality parameters. LWT - Food Science and Technology, 65, 808-815
Domínguez E., Mercado José A., Quesada Miguel A., Heredia A. (1999): Pollen sporopollenin: degradation and structural elucidation. Sexual Plant Reproduction, 12, 171-178
Dong Jie, Gao Kun, Wang Kai, Xu Xiang, Zhang Hongcheng (2015): Cell Wall Disruption of Rape Bee Pollen Treated with Combination of Protamex Hydrolysis and Ultrasonication. Food Research International, 75, 123-130
Fambrini M., Michelotti V., Pugliesi C. (2010): Orange, yellow and white-cream: inheritance of carotenoid-based colour in sunflower pollen. Plant Biology, 12, 197-205
Feás Xesús, Vázquez-Tato M. Pilar, Estevinho Leticia, Seijas Julio A., Iglesias Antonio (2012): Organic Bee Pollen: Botanical Origin, Nutritional Value, Bioactive Compounds, Antioxidant Activity and Microbiological Quality. Molecules, 17, 8359-8377
Heslop-Harrison J., Heslop-Harrison Y. (1991): Structural and functional variation in pollen intines. In: Blackmore S., Barnes S.H. (eds): Pollen and Spores: Patterns of Diversification. Oxford, Clarendon Press: 331–343.
Hourant Pierre, Baeten Vincent, Morales Maria T., Meurens Marc, Aparicio Ramon (2016): Oil and Fat Classification by Selected Bands of Near-Infrared Spectroscopy. Applied Spectroscopy, 54, 1168-1174
Hudz N., Ivanova R., Brindza J., Grygorieva O., Schubertová Z., Ivanišová E. (2017): Approaches to the determination of antioxidant activity of extracts from bee bread and safflower leaves and flowers. Potravinárstvo: Slovak Journal of Food Sciences, 11: 480–488.
Human Hannelie, Nicolson Sue W. (2006): Nutritional content of fresh, bee-collected and stored pollen of Aloe greatheadii var. davyana (Asphodelaceae). Phytochemistry, 67, 1486-1492
Ibrahim M., Alaam M., El-Haes H., Jalbout A.F., de Leon A. (2006): Analysis of the structure and vibrational spectra of glucose and fructose, Elética, 31: 15–21.
Isidorov V.A., Isidorova A.G., Sczczepaniak L., Czyżewska U. (2009): Gas chromatographic–mass spectrometric investigation of the chemical composition of beebread. Food Chemistry, 115, 1056-1063
Ivanišová Eva, Kačániová Miroslava, Frančáková Helena, Petrová Jana, Hutková Jana, Brovarskyi Valeryii, Velychko Serhii, Adamchuk Leonora, Schubertová Zuzana, Musilová Janette (2015): Bee bread - perspective source of bioactive compounds for future. Potravinarstvo, 9, -
Kačuráková M, Wellner N, Ebringerová A, Hromádková Z, Wilson R.H, Belton P.S (1999): Characterisation of xylan-type polysaccharides and associated cell wall components by FT-IR and FT-Raman spectroscopies. Food Hydrocolloids, 13, 35-41
Kedare Sagar B., Singh R. P. (2011): Genesis and development of DPPH method of antioxidant assay. Journal of Food Science and Technology, 48, 412-422
Kieliszek Marek, Piwowarek Kamil, Kot Anna M., Błażejak Stanisław, Chlebowska-Śmigiel Anna, Wolska Iwona (2018): Pollen and bee bread as new health-oriented products: A review. Trends in Food Science & Technology, 71, 170-180
Komosinska-Vassev Katarzyna, Olczyk Pawel, Kaźmierczak Justyna, Mencner Lukasz, Olczyk Krystyna (2015): Bee Pollen: Chemical Composition and Therapeutic Application. Evidence-Based Complementary and Alternative Medicine, 2015, 1-6
Krinsky Norman I. (2001): Carotenoids as antioxidants. Nutrition, 17, 815-817
Kročko M., Čanigová M., Bezeková J., Lavová M., Haščík P., Ducková V. (2012). Effect of nutrition with propolis and bee pollen supplements on bacteria colonization pattern in gastrointestinal tract of broiler chickens. Scientific Papers Animal Science and Biotechnologies, 45: 63–67.
Kyselka Jan, Bleha Roman, Dragoun Miroslav, Bialasová Kristýna, Horáčková Šárka, Schätz Martin, Sluková Marcela, Filip Vladimír, Synytsya Andriy (2018): Antifungal Polyamides of Hydroxycinnamic Acids from Sunflower Bee Pollen. Journal of Agricultural and Food Chemistry, 66, 11018-11026
Leja M., Mareczek A., Wyżgolik G., Klepacz-Baniak J., Czekońska K. (2007): Antioxidative properties of bee pollen in selected plant species. Food Chemistry, 100, 237-240
Margreth M., Schlink R., Steinbach A. (2010). Water determination by Karl Fischer titration. In: Gad S. (Ed.). Pharmaceutical Sciences Encyclopedia: Drug Discovery, Development, and Manufacturing. New Jersey, John Wiley & Sons, Inc.:1–34.
Mathlouthi Mohamed (2001): Water content, water activity, water structure and the stability of foodstuffs. Food Control, 12, 409-417
Miyazawa Mitsuhiro, Sonoyama Masashi (1998): Second Derivative near Infrared Studies on the Structural Characterisation of Proteins. Journal of Near Infrared Spectroscopy, 6, A253-A257
Morais Margarida, Moreira Leandro, Feás Xesús, Estevinho Leticia M. (2011): Honeybee-collected pollen from five Portuguese Natural Parks: Palynological origin, phenolic content, antioxidant properties and antimicrobial activity. Food and Chemical Toxicology, 49, 1096-1101
Morgano Marcelo A., Milani Raquel F., Martins Márcia C.T., Rodriguez-Amaya Delia B. (2011): Determination of water content in Brazilian honeybee-collected pollen by Karl Fischer titration. Food Control, 22, 1604-1608
Moţ Augustin Cătălin, Soponar Florin, Sârbu Costel (2010): Multivariate analysis of reflectance spectra from propolis: Geographical variation in Romanian samples. Talanta, 81, 1010-1015
Re Roberta, Pellegrini Nicoletta, Proteggente Anna, Pannala Ananth, Yang Min, Rice-Evans Catherine (1999): Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231-1237
Jorgensen James H., Ferraro Mary Jane (2009): Antimicrobial Susceptibility Testing: A Review of General Principles and Contemporary Practices. Clinical Infectious Diseases, 49, 1749-1755
Rice-Evans Catherine A., Miller Nicholas J., Paganga George (1996): Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology and Medicine, 20, 933-956
RICHARD D, KEFI K, BARBE U, BAUSERO P, VISIOLI F (2008): Polyunsaturated fatty acids as antioxidants. Pharmacological Research, 57, 451-455
Robert Christine, Cadet Frederic (1998): Analysis of Near-Infrared Spectra of Some Carbohydrates. Applied Spectroscopy Reviews, 33, 253-266
Roggo Yves, Chalus Pascal, Maurer Lene, Lema-Martinez Carmen, Edmond Aurélie, Jent Nadine (2007): A review of near infrared spectroscopy and chemometrics in pharmaceutical technologies. Journal of Pharmaceutical and Biomedical Analysis, 44, 683-700
Rzepecka-Stojko Anna, Stojko Jerzy, Kurek-Górecka Anna, Górecki Michał, Kabała-Dzik Agata, Kubina Robert, Moździerz Aleksandra, Buszman Ewa (2015): Polyphenols from Bee Pollen: Structure, Absorption, Metabolism and Biological Activity. Molecules, 20, 21732-21749
Sagona Simona, Bozzicolonna Rachele, Nuvoloni Roberta, Cilia Giovanni, Torracca Beatrice, Felicioli Antonio (2017): Water activity of fresh bee pollen and mixtures of bee pollen-honey of different botanical origin. LWT, 84, 595-600
Schulz Hartwig, Baranska Malgorzata (2007): Identification and quantification of valuable plant substances by IR and Raman spectroscopy. Vibrational Spectroscopy, 43, 13-25
Schulz H., Baranska M., Baranski R. (2005): Potential of NIR-FT-Raman spectroscopy in natural carotenoid analysis. Biopolymers, 77, 212-221
Soukupova J., Rock B. N., Albrechtova J. (2010): Spectral characteristics of lignin and soluble phenolics in the near infrared- a comparative study. International Journal of Remote Sensing, 23, 3039-3055
Söderholm Stefan, Roos Yrjö H., Meinander Niklas, Hotokka Matti (1999): Raman spectra of fructose and glucose in the amorphous and crystalline states. Journal of Raman Spectroscopy, 30, 1009-1018<1009::AID-JRS436>3.0.CO;2-#
Synytsya A., Synytsya A., Bleha R., Gróf J., Nôžková J., Ostrovský R., Čopíková J., Brindza J. (2010): New trends in classification and quality estimation of bee pollen. Potravinárstvo, 4: 236–245.
Synytsya A., Synytsya A., Bleha R., Brindza J., Ostrovský R., Čopíková J., Nôžková J. (2011): Morphologic and spectroscopic analysis of sunflower (Helianthus annuus L.) bee pollen. Potravinárstvo, 5: 308–313.
Tavdidishvili D., Khutsidze T., Pkhakadze M., Vanidze M., Kalandia A. (2014): Flavonoids in Georgian bee bread and bee pollen. Journal of Chemistry and Chemical Engineering, 8: 676–681.
Urcan A., Mărghitaş L.A., Dezmirean D.S., Bobiş O., Bonta V., Mureşan C.I., MĂrgĂoan R. (2017): Chemical Composition and Biological Activities of Beebread-Review. Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies, 74: 6–14.
Vaissière Bernard E., Vinson S. Bradleigh (1994): Pollen morphology and its effect on pollenl collection by honey bees, Apis Mellifera L. (Hymenoptera: Apidae), with special Reference to Upland Cotton, Gossypium Hirsutum L. (Malvaceae). Grana, 33, 128-138
Vlachos N., Skopelitis Y., Psaroudaki M., Konstantinidou V., Chatzilazarou A., Tegou E. (2006): Applications of Fourier transform-infrared spectroscopy to edible oils. Analytica Chimica Acta, 573-574, 459-465
Weyer L. G. (2006): Near-Infrared Spectroscopy of Organic Substances. Applied Spectroscopy Reviews, 21, 1-43
Workman Jerome J. (1996): Interpretive Spectroscopy for Near Infrared. Applied Spectroscopy Reviews, 31, 251-320
Wroblewska A., Warakomska Z., Kaminska M. (2010): The pollen spectrum of bee bread from the Lublin region (Poland). Journal of Apicultural Science 54: 81–89.
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