Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakii

https://doi.org/10.17221/206/2009-CJFSCitation:

Hochel I., Škvor J. (2009): Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakii. Czech J. Food Sci., 27: 66-74.

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An indirect competitive enzyme immunoassay of Enterobacter sakazakii has been developed. The rabbit polyclonal antibodies to heat-labile or heat-stable antigen of the type strain E. sakazakii CNCTC 5739T were prepared for these purposes. The detection limits of enzyme immunoassays were within the range 0.6–14.4 × 105 cells/ml. Antibodies raised to heat-labile antigen were serotype-specific. Although they contain non-specific IgG fractions binding periplasmatic and cytosol proteins, the interactions of these immunoglobulins are not manifested under conditions of ELISA developed.

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