Rapid determination of theaflavins by HPLC with a new monolithic column

https://doi.org/10.17221/213/2018-CJFSCitation:Zhang J., Cui H., Jiang H., Fang L., Wang W., Su W., Xiong C. (2019): Rapid determination of theaflavins by HPLC with a new monolithic column. Czech J. Food Sci., 37: 112-119.
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The quantitative determination of four theaflavin monomers by a rapid reversed-phase high performance liquid chromatographic method was developed. A new RP-18 end-capped column with particle size 2 µm and equilibrated to 35°C in a Shimadzu temperature controller module was used. Four theaflavin monomers were successfully separated in 8 min by the new strategy, comparing to 20–85 min by HPLC in the peer literature reports. Linear gradient elution: from 92% mobile phase A (v) to 76% mobile phase A (v) during early 3 min and then 92% mobile phase A (v) till 8 min at elution flow rate 1.5 ml/min. The limits of detection and quantification were in the range of 0.1–0.3 and 0.4–1.1 mg/l. Satisfactory recoveries of theaflavin, theaflavin-3-gallate, theaflavin-3’-gallate and theaflavin-3,3’-gallate were 97.5–102.6, 98.6–102.4, 99.6–105.4, and 95.5–105.4%, respectively. The new method was applied to quantitative analysis theaflavins of tea samples, including 10 black teas, 5 oolong teas, and 5 green teas. This method is suitable for the rapid, accurate and inexpensive quantitative analysis of theaflavins under the basic detection conditions of HPLC.

References:
Asako N.K., Aya K., Yuuka U., Miho K., Tsutomu N. (2017): Specificity of tyrosinase-catalyzed synthesis of theaflavins. Journal of Molecular Catalysis B: Enzymatic, 133 (S1): 452–458.
 
Betts J.W., Kelly S.M., Haswell S.J. (2011): Antibacterial effects of theaflavin and synergy with epicatechin against clinical isolates of Acinetobacter baumannii and Stenotrophomonas maltophilia. International Journal of Antimicrobial Agents, 38, 421-425 https://doi.org/10.1016/j.ijantimicag.2011.07.006
 
Carloni Patricia, Tiano Luca, Padella Lucia, Bacchetti Tiziana, Customu Chisomo, Kay Alexander, Damiani Elisabetta (2013): Antioxidant activity of white, green and black tea obtained from the same tea cultivar. Food Research International, 53, 900-908 https://doi.org/10.1016/j.foodres.2012.07.057
 
Collier P.D., Mallows R. (1971): Estimation of theaflavins in tea by gas—liquid chromatography of their trimethylsilyl ethers. Journal of Chromatography A, 57, 19-27 https://doi.org/10.1016/0021-9673(71)80003-1
 
Chen Huadong, Parks Tiffany A., Chen Xiaoxin, Gillitt Nicholas D., Jobin Christian, Sang Shengmin (2011): Structural identification of mouse fecal metabolites of theaflavin 3,3′-digallate using liquid chromatography tandem mass spectrometry. Journal of Chromatography A, 1218, 7297-7306 https://doi.org/10.1016/j.chroma.2011.08.056
 
Yabuki Chikako, Yagi Kensuke, Nanjo Fumio (2017): Highly efficient synthesis of theaflavins by tyrosinase from mushroom and its application to theaflavin related compounds. Process Biochemistry, 55, 61-69 https://doi.org/10.1016/j.procbio.2017.02.002
 
Geiser Ryan J., Chastain Shelby E., Moss Melissa A. (2017): Regulation of Bace1 Mrna Expression in Alzheimer'S Disease by Green Tea Catechins and Black Tea Theaflavins. Biophysical Journal, 112, 362a- https://doi.org/10.1016/j.bpj.2016.11.1965
 
Honglin L.L., Tong H.R. (2016): Determination of ten components in congou black tea by HPLC. Food Science, 37(8): 97–101.
 
Hu Xuanyang, Ping Zichuan, Gan Minfeng, Tao Yunxia, Wang Liangliang, Shi Jiawei, Wu Xiexing, Zhang Wen, Yang Huilin, Xu Yaozeng, Wang Zhirong, Geng Dechun (2017): Theaflavin-3,3′-digallate represses osteoclastogenesis and prevents wear debris-induced osteolysis via suppression of ERK pathway. Acta Biomaterialia, 48, 479-488 https://doi.org/10.1016/j.actbio.2016.11.022
 
Jin Duiyan, Xu Yi, Mei Xin, Meng Qing, Gao Ying, Li Bo, Tu Youying (2013): Antiobesity and lipid lowering effects of theaflavins on high-fat diet induced obese rats. Journal of Functional Foods, 5, 1142-1150 https://doi.org/10.1016/j.jff.2013.03.011
 
Jinjin X.J., Jiang H.Y., Long D., Wang W.W., Zhang J.Y. (2014): Simultaneous multiresidue determination of theasinensins and theaflavins in tea using high performance liquid chromatography. Journal of Chinese Institute of Food Science and Technology, 14: 237–243.
 
Korir M.W., Wachira F.N., Wanyoko J.K., Ngure R.M., Khalid R. (2014): The fortification of tea with sweeteners and milk and its effect on in vitro antioxidant potential of tea product and glutathione levels in an animal model. Food Chemistry, 145, 145-153 https://doi.org/10.1016/j.foodchem.2013.08.016
 
Lee Bee-Lan, Ong Choon-Nam (2000): Comparative analysis of tea catechins and theaflavins by high-performance liquid chromatography and capillary electrophoresis. Journal of Chromatography A, 881, 439-447 https://doi.org/10.1016/S0021-9673(00)00215-6
 
Li D.X., Wan X.C., Liu L.H., Xia T. (2004): HPLC quantitation of theaflavins in tea pitments. Journal of Tea Science, 24(2): 124–128.
 
Narai K.A., Kawashima A., Uchida Y., Kawamura M., Nakayama T. (2016): Specificity of tyrosinase-catalyzed synthesis of theaflavins. Journal of Molecular Catalysis B: Enzymatic, 133: S452–S458.
 
Ouyang Qin, Yang Yongcun, Wu Jizhong, Liu Zhengquan, Chen XiaoHong, Dong Chunwang, Chen Quansheng, Zhang Zhengzu, Guo Zhiming (2019): Rapid sensing of total theaflavins content in black tea using a portable electronic tongue system coupled to efficient variables selection algorithms. Journal of Food Composition and Analysis, 75, 43-48 https://doi.org/10.1016/j.jfca.2018.09.014
 
Glisan Shannon L., Grove Kimberly A., Yennawar Neela H., Lambert Joshua D. (2017): Inhibition of pancreatic lipase by black tea theaflavins: Comparative enzymology and in silico modeling studies. Food Chemistry, 216, 296-300 https://doi.org/10.1016/j.foodchem.2016.08.052
 
Sharma Kapil, Bari Shamsher S., Singh Harsh P. (2009): Biotransformation of tea catechins into theaflavins with immobilized polyphenol oxidase. Journal of Molecular Catalysis B: Enzymatic, 56, 253-258 https://doi.org/10.1016/j.molcatb.2008.05.016
 
Sun Lijun, Warren Fredrick J., Netzel Gabriele, Gidley Michael J. (2016): 3 or 3′-Galloyl substitution plays an important role in association of catechins and theaflavins with porcine pancreatic α-amylase: The kinetics of inhibition of α-amylase by tea polyphenols. Journal of Functional Foods, 26, 144-156 https://doi.org/10.1016/j.jff.2016.07.012
 
Tao Wuqun, Zhou Zhiguang, Zhao Bin, Wei Tongyu (2016): Simultaneous determination of eight catechins and four theaflavins in green, black and oolong tea using new HPLC–MS–MS method. Journal of Pharmaceutical and Biomedical Analysis, 131, 140-145 https://doi.org/10.1016/j.jpba.2016.08.020
 
Nandy Chatterjee Trisita, Banerjee Roy Runu, Tudu Bipan, Pramanik Panchanan, Deka Himangshu, Tamuly Pradip, Bandyopadhyay Rajib (2017): Detection of theaflavins in black tea using a molecular imprinted polyacrylamide-graphite nanocomposite electrode. Sensors and Actuators B: Chemical, 246, 840-847 https://doi.org/10.1016/j.snb.2017.02.139
 
Wang Kunbo, Liu Zhonghua, Huang Jian-an, Dong Xinrong, Song Lubing, Pan Yu, liu Fang (2008): Preparative isolation and purification of theaflavins and catechins by high-speed countercurrent chromatography. Journal of Chromatography B, 867, 282-286 https://doi.org/10.1016/j.jchromb.2008.04.005
 
Wang Yuanyuan, Yang Xiaorong, Li Kaikai, Li Chengren, Li Linlin, Li Jiaxian, Huang Hualin, He Yumei, Ye Chuangxing, Song Xiaohong (2010): Simultaneous determination of theanine, gallic acid, purine alkaloids, catechins, and theaflavins in black tea using HPLC. International Journal of Food Science & Technology, 45, 1263-1269 https://doi.org/10.1111/j.1365-2621.2010.02266.x
 
Wright Louwrance Peter, Aucamp Jean Pieter, Apostolides Zeno (2001): Analysis of black tea theaflavins by non-aqueous capillary electrophoresis. Journal of Chromatography A, 919, 205-213 https://doi.org/10.1016/S0021-9673(01)00762-2
 
Xia T., Gao L.P. (1999): A study on the determination of color separation of black tea and its effect on quality. China Tea Processing, 2: 42–45.
 
Xu Lujing, Xia Guobin, Luo Zisheng, Liu Songbai (2019): UHPLC analysis of major functional components in six types of Chinese teas: Constituent profile and origin consideration. LWT, 102, 52-57 https://doi.org/10.1016/j.lwt.2018.12.008
 
Xu Yong-Quan, Liu Pan-Pan, Shi John, Gao Ying, Wang Qiu-Shuang, Yin Jun-Feng (2018): Quality development and main chemical components of Tieguanyin oolong teas processed from different parts of fresh shoots. Food Chemistry, 249, 176-183 https://doi.org/10.1016/j.foodchem.2018.01.019
 
Zu Mian, Yang Fan, Zhou Weiling, Liu Ailin, Du Guanhua, Zheng Lishu (2012): In vitro anti-influenza virus and anti-inflammatory activities of theaflavin derivatives. Antiviral Research, 94, 217-224 https://doi.org/10.1016/j.antiviral.2012.04.001
 
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