Rapid determination of theaflavins by HPLC with a new monolithic column

https://doi.org/10.17221/213/2018-CJFSCitation:Zhang J., Cui H., Jiang H., Fang L., Wang W., Su W., Xiong C. (2019): Rapid determination of theaflavins by HPLC with a new monolithic column. Czech J. Food Sci., 37: 112-119.
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The quantitative determination of four theaflavin monomers by a rapid reversed-phase high performance liquid chromatographic method was developed. A new RP-18 end-capped column with particle size 2 µm and equilibrated to 35°C in a Shimadzu temperature controller module was used. Four theaflavin monomers were successfully separated in 8 min by the new strategy, comparing to 20–85 min by HPLC in the peer literature reports. Linear gradient elution: from 92% mobile phase A (v) to 76% mobile phase A (v) during early 3 min and then 92% mobile phase A (v) till 8 min at elution flow rate 1.5 ml/min. The limits of detection and quantification were in the range of 0.1–0.3 and 0.4–1.1 mg/l. Satisfactory recoveries of theaflavin, theaflavin-3-gallate, theaflavin-3’-gallate and theaflavin-3,3’-gallate were 97.5–102.6, 98.6–102.4, 99.6–105.4, and 95.5–105.4%, respectively. The new method was applied to quantitative analysis theaflavins of tea samples, including 10 black teas, 5 oolong teas, and 5 green teas. This method is suitable for the rapid, accurate and inexpensive quantitative analysis of theaflavins under the basic detection conditions of HPLC.

Asako N.K., Aya K., Yuuka U., Miho K., Tsutomu N. (2017): Specificity of tyrosinase-catalyzed synthesis of theaflavins. Journal of Molecular Catalysis B: Enzymatic, 133 (S1): 452–458.
Betts J.W., Kelly S.M., Haswell S.J. (2011): Antibacterial effects of theaflavin and synergy with epicatechin against clinical isolates of Acinetobacter baumannii and Stenotrophomonas maltophilia. International Journal of Antimicrobial Agents, 38, 421-425 https://doi.org/10.1016/j.ijantimicag.2011.07.006
Carloni Patricia, Tiano Luca, Padella Lucia, Bacchetti Tiziana, Customu Chisomo, Kay Alexander, Damiani Elisabetta (2013): Antioxidant activity of white, green and black tea obtained from the same tea cultivar. Food Research International, 53, 900-908 https://doi.org/10.1016/j.foodres.2012.07.057
Collier P.D., Mallows R. (1971): Estimation of theaflavins in tea by gas—liquid chromatography of their trimethylsilyl ethers. Journal of Chromatography A, 57, 19-27 https://doi.org/10.1016/0021-9673(71)80003-1
Chen Huadong, Parks Tiffany A., Chen Xiaoxin, Gillitt Nicholas D., Jobin Christian, Sang Shengmin (2011): Structural identification of mouse fecal metabolites of theaflavin 3,3′-digallate using liquid chromatography tandem mass spectrometry. Journal of Chromatography A, 1218, 7297-7306 https://doi.org/10.1016/j.chroma.2011.08.056
Yabuki Chikako, Yagi Kensuke, Nanjo Fumio (2017): Highly efficient synthesis of theaflavins by tyrosinase from mushroom and its application to theaflavin related compounds. Process Biochemistry, 55, 61-69 https://doi.org/10.1016/j.procbio.2017.02.002
Geiser Ryan J., Chastain Shelby E., Moss Melissa A. (2017): Regulation of Bace1 Mrna Expression in Alzheimer'S Disease by Green Tea Catechins and Black Tea Theaflavins. Biophysical Journal, 112, 362a- https://doi.org/10.1016/j.bpj.2016.11.1965
Honglin L.L., Tong H.R. (2016): Determination of ten components in congou black tea by HPLC. Food Science, 37(8): 97–101.
Hu Xuanyang, Ping Zichuan, Gan Minfeng, Tao Yunxia, Wang Liangliang, Shi Jiawei, Wu Xiexing, Zhang Wen, Yang Huilin, Xu Yaozeng, Wang Zhirong, Geng Dechun (2017): Theaflavin-3,3′-digallate represses osteoclastogenesis and prevents wear debris-induced osteolysis via suppression of ERK pathway. Acta Biomaterialia, 48, 479-488 https://doi.org/10.1016/j.actbio.2016.11.022
Jin Duiyan, Xu Yi, Mei Xin, Meng Qing, Gao Ying, Li Bo, Tu Youying (2013): Antiobesity and lipid lowering effects of theaflavins on high-fat diet induced obese rats. Journal of Functional Foods, 5, 1142-1150 https://doi.org/10.1016/j.jff.2013.03.011
Jinjin X.J., Jiang H.Y., Long D., Wang W.W., Zhang J.Y. (2014): Simultaneous multiresidue determination of theasinensins and theaflavins in tea using high performance liquid chromatography. Journal of Chinese Institute of Food Science and Technology, 14: 237–243.
Korir M.W., Wachira F.N., Wanyoko J.K., Ngure R.M., Khalid R. (2014): The fortification of tea with sweeteners and milk and its effect on in vitro antioxidant potential of tea product and glutathione levels in an animal model. Food Chemistry, 145, 145-153 https://doi.org/10.1016/j.foodchem.2013.08.016
Lee Bee-Lan, Ong Choon-Nam (2000): Comparative analysis of tea catechins and theaflavins by high-performance liquid chromatography and capillary electrophoresis. Journal of Chromatography A, 881, 439-447 https://doi.org/10.1016/S0021-9673(00)00215-6
Li D.X., Wan X.C., Liu L.H., Xia T. (2004): HPLC quantitation of theaflavins in tea pitments. Journal of Tea Science, 24(2): 124–128.
Narai K.A., Kawashima A., Uchida Y., Kawamura M., Nakayama T. (2016): Specificity of tyrosinase-catalyzed synthesis of theaflavins. Journal of Molecular Catalysis B: Enzymatic, 133: S452–S458.
Ouyang Qin, Yang Yongcun, Wu Jizhong, Liu Zhengquan, Chen XiaoHong, Dong Chunwang, Chen Quansheng, Zhang Zhengzu, Guo Zhiming (2019): Rapid sensing of total theaflavins content in black tea using a portable electronic tongue system coupled to efficient variables selection algorithms. Journal of Food Composition and Analysis, 75, 43-48 https://doi.org/10.1016/j.jfca.2018.09.014
Glisan Shannon L., Grove Kimberly A., Yennawar Neela H., Lambert Joshua D. (2017): Inhibition of pancreatic lipase by black tea theaflavins: Comparative enzymology and in silico modeling studies. Food Chemistry, 216, 296-300 https://doi.org/10.1016/j.foodchem.2016.08.052
Sharma Kapil, Bari Shamsher S., Singh Harsh P. (2009): Biotransformation of tea catechins into theaflavins with immobilized polyphenol oxidase. Journal of Molecular Catalysis B: Enzymatic, 56, 253-258 https://doi.org/10.1016/j.molcatb.2008.05.016
Sun Lijun, Warren Fredrick J., Netzel Gabriele, Gidley Michael J. (2016): 3 or 3′-Galloyl substitution plays an important role in association of catechins and theaflavins with porcine pancreatic α-amylase: The kinetics of inhibition of α-amylase by tea polyphenols. Journal of Functional Foods, 26, 144-156 https://doi.org/10.1016/j.jff.2016.07.012
Tao Wuqun, Zhou Zhiguang, Zhao Bin, Wei Tongyu (2016): Simultaneous determination of eight catechins and four theaflavins in green, black and oolong tea using new HPLC–MS–MS method. Journal of Pharmaceutical and Biomedical Analysis, 131, 140-145 https://doi.org/10.1016/j.jpba.2016.08.020
Nandy Chatterjee Trisita, Banerjee Roy Runu, Tudu Bipan, Pramanik Panchanan, Deka Himangshu, Tamuly Pradip, Bandyopadhyay Rajib (2017): Detection of theaflavins in black tea using a molecular imprinted polyacrylamide-graphite nanocomposite electrode. Sensors and Actuators B: Chemical, 246, 840-847 https://doi.org/10.1016/j.snb.2017.02.139
Wang Kunbo, Liu Zhonghua, Huang Jian-an, Dong Xinrong, Song Lubing, Pan Yu, liu Fang (2008): Preparative isolation and purification of theaflavins and catechins by high-speed countercurrent chromatography. Journal of Chromatography B, 867, 282-286 https://doi.org/10.1016/j.jchromb.2008.04.005
Wang Yuanyuan, Yang Xiaorong, Li Kaikai, Li Chengren, Li Linlin, Li Jiaxian, Huang Hualin, He Yumei, Ye Chuangxing, Song Xiaohong (2010): Simultaneous determination of theanine, gallic acid, purine alkaloids, catechins, and theaflavins in black tea using HPLC. International Journal of Food Science & Technology, 45, 1263-1269 https://doi.org/10.1111/j.1365-2621.2010.02266.x
Wright Louwrance Peter, Aucamp Jean Pieter, Apostolides Zeno (2001): Analysis of black tea theaflavins by non-aqueous capillary electrophoresis. Journal of Chromatography A, 919, 205-213 https://doi.org/10.1016/S0021-9673(01)00762-2
Xia T., Gao L.P. (1999): A study on the determination of color separation of black tea and its effect on quality. China Tea Processing, 2: 42–45.
Xu Lujing, Xia Guobin, Luo Zisheng, Liu Songbai (2019): UHPLC analysis of major functional components in six types of Chinese teas: Constituent profile and origin consideration. LWT, 102, 52-57 https://doi.org/10.1016/j.lwt.2018.12.008
Xu Yong-Quan, Liu Pan-Pan, Shi John, Gao Ying, Wang Qiu-Shuang, Yin Jun-Feng (2018): Quality development and main chemical components of Tieguanyin oolong teas processed from different parts of fresh shoots. Food Chemistry, 249, 176-183 https://doi.org/10.1016/j.foodchem.2018.01.019
Zu Mian, Yang Fan, Zhou Weiling, Liu Ailin, Du Guanhua, Zheng Lishu (2012): In vitro anti-influenza virus and anti-inflammatory activities of theaflavin derivatives. Antiviral Research, 94, 217-224 https://doi.org/10.1016/j.antiviral.2012.04.001
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