Fractionation, antioxidant and inhibitory activity of Thai mango seed kernel extracts
Chaianun Namngam, Praphan Pinsirodom, Supakorn Boonyuenhttps://doi.org/10.17221/225/2017-CJFSCitation:Namngam C., Pinsirodom P., Boonyuen S. (2018): Fractionation, antioxidant and inhibitory activity of Thai mango seed kernel extracts. Czech J. Food Sci., 36: 8-15.
Mango seed kernel extracts (MSKE) from Kaew and Choke-Anan mango cultivars were fractionated using Sephadex LH-20 column chromatography. Antioxidant activity and the inhibitory effects on tyrosinase, 5-lipoxygenase, hyaluronidase and α-glucosidase of MSKE fractions were evaluated. The key components of polyphenol, found in the most active fraction, were identified using LC-ESI-MS. Three major isolates were obtained from both cultivars of the MSKE with the absorbance being higher for Choke-Anan than Kaew. Fraction 3 of MSKE from both cultivars showed significantly (P ≤ 0.05) higher antioxidant activity and 5-lipoxygenase, hyaluronidase and α-glucosidase inhibitory activity with Choke-Anan more effective than Kaew. Six different polyphenols were found in fraction 3 of both cultivars. These were tri-o-galloyl-glucoside, tetra-o-galloyl-glucoside, maclurin tri-o-galloyl-glucoside, penta-o-galloyl-glucoside, hexa-o-galloyl-glucoside, and hepta-o-galloyl-glucoside. In the MSKE from Choke-Anan, hexa-o-galloyl-glucoside, and tetra-o-galloyl-glucoside were the two major components, whereas in the MSKE from Kaew tetra-o-galloyl-glucoside was the only major component. The results indicate that MSKE is a suitable by-product that could be utilised for adding value to the mango processing industry and could represent a valuable input into functional foods and pharmaceutical production.Keywords:
tyrosinase; 5-lipoxygenase; hyaluronidase; α-glucosidase galloyl-glucoside; Mangifera indica extractReferences:
Abdullah A.H., Mohammed A.S., Abdullah R. (2015): Iden¬tification and quantification of phenolic compound in Mangifera indica waterlily kernel and their free radical scarvening activity. Journal of Advanced Agricultural Technology, 2: 1–7.Abdalla Ahmed E.M., Darwish Saeid M., Ayad Eman H.E., El-Hamahmy Reham M. (2007): Egyptian mango by-product 1. Compositional quality of mango seed kernel. Food Chemistry, 103, 1134-1140 https://doi.org/10.1016/j.foodchem.2006.10.017ALITONOU G, AVLESSI F, SOHOUNHLOUE D, AGNANIET H, BESSIERE J, MENUT C (2006): Investigations on the essential oil of Cymbopogon giganteus from Benin for its potential use as an anti-inflammatory agent. International Journal of Aromatherapy, 16, 37-41 https://doi.org/10.1016/j.ijat.2006.01.001Apostolidis Emmanouil, Li Liya, Lee Chong, Seeram Navindra P. (2011): In vitro evaluation of phenolic-enriched maple syrup extracts for inhibition of carbohydrate hydrolyzing enzymes relevant to type 2 diabetes management. Journal of Functional Foods, 3, 100-106 https://doi.org/10.1016/j.jff.2011.03.003Berardini Nicolai, Carle Reinhold, Schieber Andreas (2004): Characterization of gallotannins and benzophenone derivatives from mango (Mangifera indica L. cv. ?Tommy Atkins?) peels, pulp and kernels by high-performance liquid chromatography/electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry, 18, 2208-2216 https://doi.org/10.1002/rcm.1611Dube Mark, Zunker Katy, Neidhart Sybille, Carle Reinhold, Steinhart Hans, Paschke Angelika (2004): Effect of Technological Processing on the Allergenicity of Mangoes ( Mangifera indica L.). Journal of Agricultural and Food Chemistry, 52, 3938-3945 https://doi.org/10.1021/jf030792rElsenhans B., Caspary W.F. (1987): Absorption of carbo¬hydrates. In: Capary W.F. (ed.): Structure and Function of the Small Intestine. Amsterdam Excerptia Medica, 139–159.Ghafoor Kashif (2009): Optimization of Ultrasound Assisted Extraction of Phenolic Compounds and Antioxidants from Grape Peel through Response Surface Methodology. Journal of the Korean Society for Applied Biological Chemistry, 52, 295-300 https://doi.org/10.3839/jksabc.2009.052Girish K., Kemparaju K. (2005): Inhibition of Naja naja venom hyaluronidase by plant derived bioactive compo¬nents and polysaccharide. Biochemistry, 70: 1145–1150.Khammuang S., Sarnthima K. (2011): Antioxidant and an¬tibacterial activities of selected varieties of Thai mango seed extract. Pakistan Journal of Pharmaceutical Sci¬ences, 24: 37–42.Kim You Jung, Kang Ki Sung, Yokozawa Takako (2008): The anti-melanogenic effect of pycnogenol by its anti-oxidative actions. Food and Chemical Toxicology, 46, 2466-2471 https://doi.org/10.1016/j.fct.2008.04.002Kumar Vipin, Prakash Om, Kumar Sunil, Narwal Smita (2011): α-glucosidase inhibitors from plants: A natural approach to treat diabetes. Pharmacognosy Reviews, 5, 19- https://doi.org/10.4103/0973-7847.79096Larrauri J.A., Rupérez P., Borroto B., Saura-Calixto F. (1996): Mango Peels as a New Tropical Fibre: Preparation and Characterization. LWT - Food Science and Technology, 29, 729-733 https://doi.org/10.1006/fstl.1996.0113Lee K.-K., Cho J.-J., Park E.-J., Choi J.-D. (2001): Anti-elastase and anti-hyaluronidase of phenolic substance from Areca catechu as a new anti-ageing agent. International Journal of Cosmetic Science, 23, 341-346 https://doi.org/10.1046/j.0412-5463.2001.00102.xLuo Fenglei, Fu Yingying, Xiang Yu, Yan Shuxia, Hu Guibing, Huang Xuming, Huang Guodi, Sun Chongde, Li Xian, Chen Kunsong (2014): Identification and quantification of gallotannins in mango (Mangifera indica L.) kernel and peel and their antiproliferative activities. Journal of Functional Foods, 8, 282-291 https://doi.org/10.1016/j.jff.2014.03.030Maisuthisakul Pitchaon, Gordon Michael H. (2009): Antioxidant and tyrosinase inhibitory activity of mango seed kernel by product. Food Chemistry, 117, 332-341 https://doi.org/10.1016/j.foodchem.2009.04.010Maisuthisakul P. (2011): Antioxidant capacity of extracts and fractions from mango (Mangifera indica L.) seed ker¬nels. International Food Research Journal, 18: 523–528.MASUDA Toshiya, YAMASHITA Daiki, TAKEDA Yoshio, YONEMORI Shigetomo (2014): Screening for Tyrosinase Inhibitors among Extracts of Seashore Plants and Identification of Potent Inhibitors from Garcinia subelliptica. Bioscience, Biotechnology, and Biochemistry, 69, 197-201 https://doi.org/10.1271/bbb.69.197Namngam Chaianun, Pinsirodom Praphan (2017): Antioxidant properties, selected enzyme inhibition capacities, and a cosmetic cream formulation of Thai mango seed kernel extracts. Tropical Journal of Pharmaceutical Research, 16, 9- https://doi.org/10.4314/tjpr.v16i1.3Nishaa S., Vishnupriya M., Sasikumar J.M., Hephazibah P.C., Gopalakrishnan V.K. (2012): Antioxidant activity of ethanolic extract of Maranta arundinacea L. tuberous rhizomes. Asian Journal of Pharmaceutical and Clinical Research, 5: 85–88.Nithitanakool Saruth, Pithayanukul Pimolpan, Bavovada Rapepol (2009): Antioxidant and Hepatoprotective Activities of Thai Mango Seed Kernel Extract. Planta Medica, 75, 1118-1123 https://doi.org/10.1055/s-0029-1185507Park S. -H., Kim D. -S., Kim W. -G., Ryoo I. -J., Lee D. -H., Huh C. -H., Youn S. -W., Yoo I. -D., Park K. -C. (2004): Terrein: a new melanogenesis inhibitor and its mechanism. Cellular and Molecular Life Sciences, 61, 2878-2885 https://doi.org/10.1007/s00018-004-4341-3Re Roberta, Pellegrini Nicoletta, Proteggente Anna, Pannala Ananth, Yang Min, Rice-Evans Catherine (1999): Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3Resoanaivo L.H., Albrieux F., Lemaire M. (2014): Chemical constituents of peels, kernels and hulls of fruits of Mangif¬era indica Var. Hiesy and their potential valorization. Jour¬nal of Pharmacognosy and Phytochemistry, 3: 225–233.Sahasrabud Abhijit, Deodhar Manjushree (2010): Anti-hyaluronidase, Anti-elastase Activity of Garcinia indica. International Journal of Botany, 6, 299-303 https://doi.org/10.3923/ijb.2010.299.303Shimogaki , Tanaka , Tamai , Masuda (2000): In vitro and in vivo evaluation of ellagic acid on melanogenesis inhibition. International Journal of Cosmetic Science, 22, 291-303 https://doi.org/10.1046/j.1467-2494.2000.00023.xShinde U.A., Phadke A.S., Nari A.M., Mungantiwar A.A., Dikshit V.J., Saraf M.N. (1999): Membrane stabilization activity a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fito¬terapia, 70: 225–257.Singleton V.L., Lamuela-Raventos R.M. (1999): Analysis of total phenol and other oxidation substrates and antioxi¬dants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299: 152–178.Yen Gow-Chin, Chen Hui-Yin (1995): Antioxidant Activity of Various Tea Extracts in Relation to Their Antimutagenicity. Journal of Agricultural and Food Chemistry, 43, 27-32 https://doi.org/10.1021/jf00049a007