Storage stability of fermented milk with probiotic monoculture and transglutaminase
The effect of microbial transglutaminase on selected physicochemical and organoleptic characteristics and viability of probiotic bacteria in fermented milk inoculated with probiotic monoculture (Lactobacillus acidophilus LA 5 or Bifidobacterium bifidum BB 12) was analysed. Four types of samples were prepared: (1) fermented milk inoculated with Lactobacillus acidophilus LA 5, (2) fermented milk inoculated with Bifidobacterium bifidum BB 12, (3) fermented milk produced from milk previously treated with mTGase and inoculated with Lactobacillus acidophilus LA 5, (4) and fermented milk produced from milk previously treated with mTGase and inoculated with Bifidobacterium bifidum strain BB 12. The samples were analysed after the 1st, 7th and 14th day of storage at 5 ± 1°C. It has been found that the use of microbial transglutaminase for the production of fermented milk inoculated with monoculture affected its viscosity, hardness, acetaldehyde content and increased the viability of probiotic bacteria. The enzyme activity resulted in an significant decrease in the titratable acidity of the experimental products, positively affected viscosity, the viability of probiotic bacteria and the organoleptic properties of fermented milk.
Bielecka M. (1984): Dairy Starters. Technological Instructions of Starters Production. Warsaw, Poland: 31.
Buriti F.C.A., Castro I.A., Saad S.M.I. (2010): Viability of Lactobacillus acidophilus in synbiotic guava mousses and its survival under in vitro simulated gastrointestinal conditions. International Journal of Food Microbiology, 137: 121–129. https://doi.org/10.1016/j.ijfoodmicro.2009.11.030
Dmytrów I., Jasińska M., Dmytrów K. (2010): Effect of microbiological transglutaminase on selected physicochemical properties of tvarog. Italian Journal of Food Sience, 4: 449–460.
Domagała J., Wszołek M. (2008): Effect of compaction and the type of starter on the texture and susceptibility to syneresis of yogurts and bio-yoghurts from goat’s milk. Food Science Technology Qualifications, 6: 118–126.
Donkor O.N., Henriksson A., Vasiljevic T., Shah N.P. (2006): Effect of acidification on the activity of probiotics in yoghurt during cold storage. International Dairy Journal, 16: 1181–1189. https://doi.org/10.1016/j.idairyj.2005.10.008
Fernandez M.M., De Ruiz Holgado A.P., De Valdez G.F. (1998): Survival rate and enzyme activities of Lactobacillus acidophilus following frozen storage. Cryobiology, 36: 315–319. https://doi.org/10.1006/cryo.1998.2090
Korbekandi H., Mortazavian A.M., Iravani S. (2011): Technology and stability of probiotic in fermented milks. In: Shah N. (ed.): Probiotic and Prebiotic Foods: Technology, Stability and Benefits to the Human Health. New York, Nova Science Publishers Ltd.: 131–169.
Less G.J., Jago G.R. (1969): Methods for the estimation of acetaldehyde in cultured dairy products. Australian Journal of Dairy Technology, 24: 181–185.
Lorenzen P.Ch., Neve H., Mautner A., Schlimme E. (2002): Effect of enzymatic cross-linking of milk proteins on functional properties of set-style yoghurt. International Journal of Dairy Technology, 55: 152–157. https://doi.org/10.1046/j.1471-0307.2002.00065.x
Milanowić S.D., Carić M., Durić M., Iličić M., Duraković K. (2007): Physico-chemical properties of probiotic yoghurt produced with transglutaminase. Acta Periodica Technologica, 38: 45–52. https://doi.org/10.2298/APT0738045M
Mituniewicz-Małek A., Ziarno M., Dmytrów I. (2014): Incorporation of inulin and transglutaminase in fermented goat milk containing probiotic bacteria. Journal of Dairy Science, 97: 3332–3338.
Neve H., Lorenzen P.Ch., Mautner A., Schlimme E., Heller K.J. (2001): Effects of transglutaminase treatment on the production of set skim-milk yoghurt. microbiological aspects. Kieler milchwirtschaftliche Forschungsberichte, 53: 347–361.
Nowak A., Śliżewska K., Libudzisz Z. (2010): Probiotics – the history and mechanisms of action. Food Science Technology Qualifications, 4: 5–19.
Østile M.H., Helland M.H.J.A., Narvhus J.A. (2003): Growth and metabolism of selected strains of probiotic bacteria in milk. International Journal of Food Microbiology, 87: 17–27. https://doi.org/10.1016/S0168-1605(03)00044-8
Özer B., Kirmaci H.A., Öztekin Ş., Hayaloğlu A., Atamer M. (2007): Incorporation of microbial transglutaminase into non-fat yoghurt production. International Dairy Journal, 17: 199–207. https://doi.org/10.1016/j.idairyj.2006.02.007
Sady M., Domagała J., Grega T., Kalicka D. (2007): Effect of storage time on the microflora of yoghurt with seeds of amaranthus and oats. Food Science and Technology, 55: 242–250.
Tamime A.Y., Saarela M., Sondergaard A.K., Mistry V.V. (2005): Production and maintenance of viability of probiotic microorganisms in dairy product. In: Tamime A.Y. (ed.): Probiotic Dairy Products. Oxford, Blackwell Publishing Ltd.: 39–72.
Tratnik L., Bozanic R., Herceg Z., Draglic I. (2006): The quality of plain and supplemented kefir from goat’s and cow’s milk. International Journal of Dairy Technology, 59: 40–46. https://doi.org/10.1111/j.1471-0307.2006.00236.x
Usajewicz I. (2008): Microbiology of milk and its products. In: Ziajka S. (ed.): Dairying. Olsztyn, Publishing of University of Warmia and Mazury: 152–204.
Wang S., Zhu H., Lu C., Kang Z., Luo Y., Feng L., Lu X. (2012): Fermented milk supplemented with probiotics and prebiotics can effectively alter the intestinal microbiota and immunity of host animals. Journal of Dairy Science, 95: 4813–4822. https://doi.org/10.3168/jds.2012-5426
Zaręba D., Obiedziński M., Ziarno M. (2008): Comparison of volatile profile of fermented and unfermented milk by yoghurt bacteria and probiotic strains. Food Sciences and Technology, 3: 18–32.