Efficacy of chemical agents and power ultrasound on biofilms formed by Asaia spp. – spoilage bacteria in beverage industries

https://doi.org/10.17221/235/2018-CJFSCitation:Šístková I., Horsáková I., Čeřovský M., Čížková H. (2019): Efficacy of chemical agents and power ultrasound on biofilms formed by Asaia spp. – spoilage bacteria in beverage industries. Czech J. Food Sci., 37: 221-225.
download PDF

Spoilage bacteria were isolated from spoiled fruit-flavoured bottled water with a visual defect in the form of floating flocks. The bacteria were identified as Asaia lannensis using the PCR technique. The efficacy of five chemical agents commonly used in the beverage industry and of power ultrasound (PUS) on Asaia biofilms was studied. A static cultivation procedure on stainless steel plates was used and the efficacy of the chemical agents was tested in two stages. First, only the chemical agents were used. Second, the effect of the application of PUS for 1 min prior to the application of the chemical agents was tested. The most effective chemical agent was the one based on peracetic acid. Its use without PUS proved to be more effective than a combination of any of the other chemical agents with PUS. The least effective methods included the physical sanitation by PUS, the chemical agent based on a 10% solution of sodium hypochlorite and sodium hydroxide, and the chemical agent containing a 0.3% solution of chlorine dioxide.

Belessi Ch.-E.A., Gounadaki A.S., Psomas A.N., Skandamis P.N. (2011): Efficiency of different sanitation methods on Listeria monocytogenes biofilms formed under various environmental conditions. International Journal of Food Microbiology, 145: S46–S52.
Erriu Matteo, Blus Cornelio, Szmukler-Moncler Serge, Buogo Silvano, Levi Raffaello, Barbato Giulio, Madonnaripa Daniele, Denotti Gloria, Piras Vincenzo, Orrù Germano (2014): Microbial biofilm modulation by ultrasound: Current concepts and controversies. Ultrasonics Sonochemistry, 21, 15-22  https://doi.org/10.1016/j.ultsonch.2013.05.011
Florjanič Maja, Kristl Julijana (2011): The control of biofilm formation by hydrodynamics of purified water in industrial distribution system. International Journal of Pharmaceutics, 405, 16-22  https://doi.org/10.1016/j.ijpharm.2010.11.038
Fratamico P.M., Annous B.A., Gunther N.W. (eds): Biofilm in the Food and Beverage Industries (1st Ed.). Cambridge, Woodhead Publishing: 600
Horsáková I., Voldřich M., Čeřovský M., Sedláčková P., Šicnerová P., Ulbrich P. (2009): Asaia sp. as a bacterium decaying the packaged still fruit beverages. Czech Journal of Food Sciences, 27: S366–S368.
Juvonen R., Virkajärvi V., Priha O., Laitila A. (2011): Microbiological Spoilage and Safety Risks in Noon-beer Beverages Produced in a Brewery Environment. Espoo, VTT Tiedotteita-Research Centre of Finland: 107.
Kitis Mehmet (2004): Disinfection of wastewater with peracetic acid: a review. Environment International, 30, 47-55  https://doi.org/10.1016/S0160-4120(03)00147-8
Kregiel Dorota, Rygala Anna, Libudzisz Zdzislawa, Walczak Piotr, Oltuszak-Walczak Elzbieta (2012): Asaia lannensis–the spoilage acetic acid bacteria isolated from strawberry-flavored bottled water in Poland. Food Control, 26, 147-150  https://doi.org/10.1016/j.foodcont.2012.01.020
Kregiel Dorota (2013): Attachment of Asaia lannensis to materials commonly used in beverage industry. Food Control, 32, 537-542  https://doi.org/10.1016/j.foodcont.2013.01.037
Mariscal Alberto, Carnero-Varo Manuel, Gutierrez-Bedmar Mario, Garcia-Rodriguez Antonio, Fernandez-Crehuet Joaquin (2007): A fluorescent method for assessing the antimicrobial efficacy of disinfectant against Escherichia coli ATCC 35218 biofilm. Applied Microbiology and Biotechnology, 77, 233-240  https://doi.org/10.1007/s00253-007-1137-z
McDonnell Gerald, Russell A. Denver (1999): Antiseptics and Disinfectants: Activity, Action, and Resistance. Clinical Microbiology Reviews, 12, 147-179  https://doi.org/10.1128/CMR.12.1.147
Moore J. E., McCalmont M., Xu J., Millar B. C., Heaney N. (2002): Asaia sp., an Unusual Spoilage Organism of Fruit-Flavored Bottled Water. Applied and Environmental Microbiology, 68, 4130-4131  https://doi.org/10.1128/AEM.68.8.4130-4131.2002
Purkrtová S., Turoňová H., Pilchová T., Demnerová K., Pazlarová J. (2010): Resistance of Listeria monocytogenes biofilms to disinfectants 326. Czech Journal of Food Sciences, 28, 326-332  https://doi.org/10.17221/153/2010-CJFS
Sedláčková P., Čeřovský M., Horsáková I., Voldřich M. (2011): Cell surface characteristic of Asaia bogorensis – spoilage microorganism of bottled water. Czech Journal of Food Sciences, 29, 457-461  https://doi.org/10.17221/96/2011-CJFS
Simões Manuel, Simões Lúcia C., Vieira Maria J. (2010): A review of current and emergent biofilm control strategies. LWT - Food Science and Technology, 43, 573-583  https://doi.org/10.1016/j.lwt.2009.12.008
download PDF

© 2019 Czech Academy of Agricultural Sciences