Efficiency of PCR-RFLP and species-specific PCR for the identification of meat origin in dry sausages
Ivona Djurkin Kušec, Danijela Samac, Vladimir Margeta, Žarko Radišić, Dragutin Vincek, Goran Kušechttps://doi.org/10.17221/243/2016-CJFSCitation:Djurkin Kušec I., Samac D., Margeta V., Radišić Ž., Vincek D., Kušec G. (2017): Efficiency of PCR-RFLP and species-specific PCR for the identification of meat origin in dry sausages. Czech J. Food Sci., 35: 386-391.
The purpose of this investigation was the identification of chicken, beef and sheep meat in pork sausages using PCR-RFLP and PCR with pecies-specific primers. Six dry fermented pork sausages were produced by adding beef, sheep and chicken meat to each in the amount of 1 and 5%. DNA was extracted from five regions of each sausage and PCR-RFLP together with PCR using species-specific primers was performed. PCR-RFLP analysis was successful only for chicken meat, while species-specific PCR was effective for identification of chicken, eef and sheep meat in all ratios and from all regions of the sausages. The results of our study show that discovering adulteration using PCR-RFLP is suitable only for chicken meat in the investigated products, while for detection of beef and sheep meat use of species-specific oligonucleotides is more effective.Keywords:
DNA; traceability; meat species; dry fermented productReferences:
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