Rapid detection of microbial contamination in UHT milk: practical application in dairy industry 

https://doi.org/10.17221/24/2018-CJFSCitation:Kračmarová M., Stiborová H., Horáčková Š., Demnerová K. (2018): Rapid detection of microbial contamination in UHT milk: practical application in dairy industry . Czech J. Food Sci., 36: 357-364.
download PDF

Microbial quality of ultra-high temperature (UHT) milk is usually ascertained by a total bacterial count (TBC) cultivation. But this is time consuming, so there is a tendency to search for faster and simpler methods. We compared three instruments, focusing on shortening the detection time and their suitability for practical use in dairy plants. Two of them, RapiScreen Dairy 1000 and Promilite III, detect microbial contamination by measuring adenosine triphosphate bioluminescence; the third, GreenLight, is based on oxygen consumption analysis. In the laboratory experiments, samples of UHT milk, were spiked with low concentration of microorganisms and then the level of microbial contamination was evaluated using the above-mentioned instruments together with cultivation method as a control. The instruments were also applied in a dairy plant to test 182 real samples. All investigated methods determined microbial quality faster than the TBC, but in some cases false positive and false negative results were obtained. Therefore, precise testing including optimizing pre-incubation time for bacteria enrichment is needed prior to industrial use. 

de Boer E (): Methodology for detection and typing of foodborne microorganisms. International Journal of Food Microbiology, 50, 119-130  https://doi.org/10.1016/S0168-1605(99)00081-1
Boor K.J., Murphy S.C. (2005): Microbiology of market milks. In: Robinson R.K. (ed.): Dairy Microbiology Handbook: The Microbiology of Milk and Milk Products. New Jersey, John Wiley & Sons: 91–122.
Burgess S.A., Flint S.H., Lindsay D., Cox M.P., Biggs P.J. (2017): Insights into the Geobacillus stearothermophilus species based on phylogenomic principles. BMC Microbiology 17: 140. doi 10.1186/s12866-017-1047-x.
Carrascosa Conrado, Saavedra Pedro, Millán Rafael, Jaber José R., Pérez Esteban, Grau Raúl, Raposo António, Mauricio Cristina, Sanjuán Esther (2012): Monitoring of cleanliness and disinfection in dairies: Comparison of traditional microbiological and ATP bioluminescence methods. Food Control, 28, 368-373  https://doi.org/10.1016/j.foodcont.2012.05.001
Cunha A.F., Lage A.D., Pereira e Araújo M.M., Abreu C.F., Tassinari A.R., Ferraz M.A., Davenport K., Cerqueira M.M.O.P. (2014): ATP-Bioluminescence as a method to evaluated microbiological quality of UHT milk. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 66, 1909-1916  https://doi.org/10.1590/1678-7396
Felice Carmelo J, Madrid Rossana E, Olivera Juan M, Rotger Viviana I, Valentinuzzi Max E (1999): Impedance microbiology: quantification of bacterial content in milk by means of capacitance growth curves. Journal of Microbiological Methods, 35, 37-42  https://doi.org/10.1016/S0167-7012(98)00098-0
Fernandes R. (2009): Microbiology Handbook: Dairy Products. 3rd Ed. Cambridge, Royal Society of Chemistry. 1–15.
Gracias Kiev S, McKillip John L (2004): A review of conventional detection and enumeration methods for pathogenic bacteria in food. Canadian Journal of Microbiology, 50, 883-890  https://doi.org/10.1139/w04-080
Griffiths M.W. (1993): Applications of Bioluminescence in the Dairy Industry. Journal of Dairy Science, 76, 3118-3125  https://doi.org/10.3168/jds.S0022-0302(93)77651-1
Griffiths M.W. (ed.) (2010): Improving the Safety and Quality of Milk. Volume 2 : Improving Quality in Milk Products. Cambridge, Woodhead Publishing Ltd: 97–123.
Gunasekera T. S., Attfield P. V., Veal D. A. (2000): A Flow Cytometry Method for Rapid Detection and Enumeration of Total Bacteria in Milk. Applied and Environmental Microbiology, 66, 1228-1232  https://doi.org/10.1128/AEM.66.3.1228-1232.2000
Holm C., Mathiasen T., Jespersen L. (2004): A flow cytometric technique for quantification and differentiation of bacteria in bulk tank milk. Journal of Applied Microbiology, 97, 935-941  https://doi.org/10.1111/j.1365-2672.2004.02346.x
Lehotová Veronika, Petruláková Monika, Valík Ľubomír (2016): Application of a new method to control microbial quality of foods based on the detection of oxygen consumption. Acta Chimica Slovaca, 9, 19-22  https://doi.org/10.1515/acs-2016-0004
O'Mahony F. C., Papkovsky D. B. (2006): Rapid High-Throughput Assessment of Aerobic Bacteria in Complex Samples by Fluorescence-Based Oxygen Respirometry. Applied and Environmental Microbiology, 72, 1279-1287  https://doi.org/10.1128/AEM.72.2.1279-1287.2006
O’Mahony Fiach, Green Rebecca A., Baylis Chris, Fernandes Richard, Papkovsky Dmitri B. (2009): Analysis of total aerobic viable counts in samples of raw meat using fluorescence-based probe and oxygen consumption assay. Food Control, 20, 129-135  https://doi.org/10.1016/j.foodcont.2008.03.003
Papkovsky Dmitri B., Dmitriev Ruslan I. (2013): Biological detection by optical oxygen sensing. Chemical Society Reviews, 42, 8700-  https://doi.org/10.1039/c3cs60131e
Pettersson B., Lembke F., Hammer P., Stackebrandt E., Priest F.G. (1996): Bacillus sporothermodurans, a new species producing highly heat-resistant endospores. International journal of Systematic and Evolutionary Microbiology, 46: 759–764.
Squirrell D.J., Price R.L., Murphy M.J. (2002): Rapid and specific detection of bacteria using bioluminescence. Analytica Chimica Acta, 457, 109-114  https://doi.org/10.1016/S0003-2670(01)01495-7
Vilar M.J., Rodríguez-Otero J.L., Diéguez F.J., Sanjuán M.L., Yus E. (2008): Application of ATP bioluminescence for evaluation of surface cleanliness of milking equipment. International Journal of Food Microbiology, 125, 357-361  https://doi.org/10.1016/j.ijfoodmicro.2008.04.024
Wang Chunxing, Zhang Bin, Zhuang Xiaomei (2013): A biochemical system of rapidly detecting bacteria based on ATP bioluminescence technology. European Food Research and Technology, 236, 41-46  https://doi.org/10.1007/s00217-012-1854-0
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti