Microbiological quality of raw milk in the Czech Republic

https://doi.org/10.17221/25/2016-CJFSCitation:Kateřina B., Marcela V., Vladimír B., Libor K., Ivana K., Renáta K. (2016): Microbiological quality of raw milk in the Czech Republic. Czech J. Food Sci., 34: 189-196.
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The microbiological and hygienic quality of cow’s, goat’s and sheep’s milk in the Czech Republic was evaluated. Milk (230 samples) was collected on 41 farms and investigated from May 2012 to October 2014. Milk was analysed for the presence of selected groups and types of bacteria: mesophilic microorganisms (total plate count – TPC), enterococci, Enterobacteriaceae, Escherichia coli, Staphylococcus aureus, Salmonella spp., Campylobacter spp., and Listeria monocytogenes. Besides these indicators and pathogenic agents, somatic cell count was determined as one of the indicators of mammary gland health in cows. TPC ranged between 8.3 × 102 and 1.2 × 109 CFU/ml and somatic cells between 1.6 × 104 and 6.8 × 106 cells/ml. The presence of E. coli was confirmed in 86.3% of samples and the colony counts ranged from 1.0 × 101 to 4.0 × 106 CFU/ml. The presence of verotoxigenic E. coli was confirmed in 3 samples (1.3%) (cow’s milk 0%; goat’s milk 6.3%; sheep’s milk 4.4%). The presence of S. aureus was confirmed in 29.1% of the samples (cow’s milk 26.9%; goat’s milk 34.4%; sheep’s milk 39.1%), but the numbers were very low (< 5.0 × 102 CFU/ml). L. monocytogenes was confirmed in 3 examined samples (1.3%) (cow’s milk 0.6%; goat’s milk 3.1%; sheep’s milk 4.4%). Salmonella spp. and Campylobacter spp. were not detected in any of the samples tested.
References:
Altalhi Abdullah D., Hassan Sabry A. (2009): Bacterial quality of raw milk investigated by Escherichia coli and isolates analysis for specific virulence-gene markers. Food Control, 20, 913-917  https://doi.org/10.1016/j.foodcont.2009.01.005
 
Badri Samira, Filliol Ingrid, Carle Isabelle, Hassar Mohammed, Fassouane Aziz, Cohen Nozha (2009): Prevalence of virulence genes in Escherichia coli isolated from food in Casablanca (Morocco). Food Control, 20, 560-564  https://doi.org/10.1016/j.foodcont.2008.08.015
 
Bergonier Dominique, de Cr�moux Ren�e, Rupp Rachel, Lagriffoul Gilles, Berthelot Xavier (2003): Mastitis of dairy small ruminants. Veterinary Research, 34, 689-716  https://doi.org/10.1051/vetres:2003030
 
Boor K.J., Brown D.P., Murphy S.C., Kozlowski S.M., Bandler D.K. (1998): Microbiological and Chemical Quality of Raw Milk in New York State. Journal of Dairy Science, 81, 1743-1748  https://doi.org/10.3168/jds.S0022-0302(98)75742-X
 
Bufano G., Dario C., Laudadio V. (1996): The characterisation of Leccese sheep: variations of chemical composition and lactodynamographic parameters in milk as related to somatic cell counts. In: Somatic Cells and Milk of Small Ruminants. Book Series: EAAP Publication No. 77. Wageningen, Wageningen Pers: 301–304.
 
Costa Daniela, Vinué Laura, Poeta Patricia, Coelho Ana Cláudia, Matos Manuela, Sáenz Yolanda, Somalo Sergio, Zarazaga Myriam, Rodrigues Jorge, Torres Carmen (2009): Prevalence of extended-spectrum beta-lactamase-producing Escherichia coli isolates in faecal samples of broilers. Veterinary Microbiology, 138, 339-344  https://doi.org/10.1016/j.vetmic.2009.03.029
 
Chu Chishih, Yu Changyou, Lee Yanhaui, Su Yaochi (2012): Genetically divergent methicillin-resistant Staphylococcus aureus and sec-dependent mastitis of dairy goats in Taiwan. BMC Veterinary Research, 8, 39-  https://doi.org/10.1186/1746-6148-8-39
 
Decree No. 289/2007 Coll. (2007): On veterinary and sanitary requirements for animal products that are not regulated by directly applicable regulations of the European Communities. Collection of Laws 289/2007.
 
Donnelly C. (2001): Factors associated with hygienic control and quality of cheeses prepared from raw milk: a review. Bulletin of the International Dairy Federation, 369: 16–27.
 
EFSA-ECDC (2012): Scientific report of EFSA and ECDC: The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2010. EFSA Journal, 10: 2597.
 
Fagan P.K., Hornitzky M.A., Bettelheim K.A., Djordjevic S.P. (1999): Detection of shiga-like toxin (stx1 and stx2), intimin (eaeA), and enterohemorrhagic Escherichia coli (EHEC) hemolysin (EHEC hlyA) genes in animal feces by multiplex PCR. Applied and Environmental Microbiology, 65: 868–872.
 
Fthenakis G.C., El-Masannat E.T.S., Booth J.M., Jones J.E.T. (1991): Somatic cell counts of ewes' milk. British Veterinary Journal, 147, 575-581  https://doi.org/10.1016/0007-1935(91)90029-M
 
Giacometti Federica, Serraino Andrea, Finazzi Guido, Daminelli Paolo, Losio Marina N., Arrigoni Norma, Piva Silvia, Florio Daniela, Riu Raffaela, Zanoni Renato G. (2012): Sale of Raw Milk in Northern Italy: Food Safety Implications and Comparison of Different Analytical Methodologies for Detection of Foodborne Pathogens. Foodborne Pathogens and Disease, 9, 293-297  https://doi.org/10.1089/fpd.2011.1052
 
González-RodríGuez M.C., Gonzalo C., San Primitivo F., Cármenes P. (1995): Relationship Between Somatic Cell Count and lntramammary Infection of the Half Udder in Dairy Ewes. Journal of Dairy Science, 78, 2753-2759  https://doi.org/10.3168/jds.S0022-0302(95)76906-5
 
Hill Bruce, Smythe Betty, Lindsay Denise, Shepherd Joanna (2012): Microbiology of raw milk in New Zealand. International Journal of Food Microbiology, 157, 305-308  https://doi.org/10.1016/j.ijfoodmicro.2012.03.031
 
. Ibtisam E.M. El Zubeir, . Mahboba I.A. Ahmed (2007): The Hygienic Quality of Raw Milk Produced by Some Dairy Farms in Khartoum State, Sudan. Research Journal of Microbiology, 2, 988-991  https://doi.org/10.3923/jm.2007.988.991
 
Jakobsen Ragnhild Aakre, Heggebø Ragna, Sunde Elin Bekvik, Skjervheim Magne (2011): Staphylococcus aureus and Listeria monocytogenes in Norwegian raw milk cheese production. Food Microbiology, 28, 492-496  https://doi.org/10.1016/j.fm.2010.10.017
 
Jamali Hossein, Paydar Mohammadjavad, Radmehr Behrad, Ismail Salmah, Dadrasnia Arezoo (2015): Prevalence and antimicrobial resistance of Staphylococcus aureus isolated from raw milk and dairy products. Food Control, 54, 383-388  https://doi.org/10.1016/j.foodcont.2015.02.013
 
Jayarao B.M., Donaldson S.C., Straley B.A., Sawant A.A., Hegde N.V., Brown J.L. (2006): A Survey of Foodborne Pathogens in Bulk Tank Milk and Raw Milk Consumption Among Farm Families in Pennsylvania. Journal of Dairy Science, 89, 2451-2458  https://doi.org/10.3168/jds.S0022-0302(06)72318-9
 
Jørgensen H.J., Mørk T., Høgåsen H.R., Rørvik L.M. (2005): Enterotoxigenic Staphylococcus aureus in bulk milk in Norway. Journal of Applied Microbiology, 99, 158-166  https://doi.org/10.1111/j.1365-2672.2005.02569.x
 
Karpíšková R., Koláčková I., Vyletělová M., Janštová B. (2011): Studie „Mléčné automaty“ – nálezy původců alimentárních onemocnění v syrovém mléce. Zprávy centra epidemiologie a mikrobiologie, 20/ 6: 212–214.
 
Lundén J., Tolvanen R., Korkeala H. (2004): Human Listeriosis Outbreaks Linked to Dairy Products in Europe. Journal of Dairy Science, 87, E6-E12  https://doi.org/10.3168/jds.S0022-0302(04)70056-9
 
Lyytikäinen Outi, Autio Tiina, Maijala Riitta, Ruutu Petri, Honkanen‐Buzalski Tuula, Miettinen Maria, Hatakka Maija, Mikkola Janne, Anttila Veli‐Jukka, Johansson Tuula, Rantala Leila, Aalto Tuula, Korkeala Hannu, Siitonen Anja (2000): An Outbreak of Listeria Monocytogenes Serotype 3a Infections from Butter in Finland. The Journal of Infectious Diseases, 181, 1838-1841  https://doi.org/10.1086/315453
 
Martineau F., Picard F.J., Roy P.H., Ouellette M., Bergeron M.G. (1998): Species-specific and ubiquitous DNA-based assays for rapid identification of Staphylococcus aureus. Journal of Clinical Microbiology, 36: 618–623.
 
Muehlherr J.E., Zweifel C., Corti S., Blanco J.E., Stephan R. (2003): Microbiological Quality of Raw Goat's and Ewe's Bulk-Tank Milk in Switzerland. Journal of Dairy Science, 86, 3849-3856  https://doi.org/10.3168/jds.S0022-0302(03)73992-7
 
Oliver Stephen P., Boor Kathryn J., Murphy Steven C., Murinda Shelton E. (2009): Food Safety Hazards Associated with Consumption of Raw Milk. Foodborne Pathogens and Disease, 6, 793-806  https://doi.org/10.1089/fpd.2009.0302
 
Pyz-Łukasik R., Paszkiewicz W., Tatara M.R., Brodzki P., Bełkot Z. (2015): Microbiological quality of milk sold directly from producers to consumers. Journal of Dairy Science, 98, 4294-4301  https://doi.org/10.3168/jds.2014-9187
 
Regulation (EC) No 853/2004 of The European Parliament and of The Council of 29 April 2004 laying down specific hygiene rules for on the hygiene of foodstuffs. Official Journal of the European Union, L 139/55.
 
Ruusunen Marjo, Salonen Marleena, Pulkkinen Hanna, Huuskonen Marianne, Hellström Sanna, Revez Joana, Hänninen Marja-Liisa, Fredriksson-Ahomaa Maria, Lindström Miia (2013): Pathogenic Bacteria in Finnish Bulk Tank Milk. Foodborne Pathogens and Disease, 10, 99-106  https://doi.org/10.1089/fpd.2012.1284
 
Spanu Vincenzo, Scarano Christian, Virdis Salvatore, Melito Sara, Spanu Carlo, De Santis Enrico Pietro Luigi (2013): Population Structure of Staphylococcus aureus Isolated from Bulk Tank Goat's Milk. Foodborne Pathogens and Disease, 10, 310-315  https://doi.org/10.1089/fpd.2012.1356
 
Van Kessel J.S., Karns J.S., Gorski L., McCluskey B.J., Perdue M.L. (2004): Prevalence of Salmonellae, Listeria monocytogenes, and Fecal Coliforms in Bulk Tank Milk on US Dairies. Journal of Dairy Science, 87, 2822-2830  https://doi.org/10.3168/jds.S0022-0302(04)73410-4
 
Verraes C., Vlaemynck G., Van Weyenberg S., De Zutter L., Daube G., Sindic M., Uyttendaele M., Herman L. (2015): A review of the microbiological hazards of dairy products made from raw milk. International Dairy Journal, 50, 32-44  https://doi.org/10.1016/j.idairyj.2015.05.011
 
Vyletělová M., Genčurová V. (2007): Comparison of food pathogens occurrence in raw milk between ecological and conventional farms. In: Proceedings Congress HELEXPO, Mar 9–11, 2007, Thessaloniki, Greece. III: 339–343.
 
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