Development of a triplex real-time PCR for simultaneous detection of allergenic ingredients in processed food W., Zhao Y., Xu Q., Chen Q. (2018): Development of a triplex real-time PCR for simultaneous detection of allergenic ingredients in processed food. Czech J. Food Sci., 36: 22-27.
download PDF
SYBR Green real-time or quantitative PCR (Q-PCR) is a suitable system in which to establish a multiplex method to detect allergenic ingredients in food. In this study, a triplex Q-PCR method was developed to detect trace amounts of peanut, soybean and sesame in processed food. Specific PCR primer sets were designed and the concentration of the primers used in the triplex PCR was optimised. The triplex method showed high specificity and sensitivity which were similar to those of the simplex method, and it was applied for the detection of allergenic ingredients in commercially available processed food. The results demonstrate that the developed triplex Q-PCR is a quick, reliable and efficient method for the detection of allergenic ingredients in processed food.
Agrimonti Caterina, Pirondini Andrea, Marmiroli Marta, Marmiroli Nelson (2015): A quadruplex PCR (qxPCR) assay for adulteration in dairy products. Food Chemistry, 187, 58-64
BOUSQUET J, LOCKEY R, MALLING H (1998): Allergen immunotherapy: Therapeutic vaccines for allergic diseases A WHO position paper☆☆☆★★★♢. Journal of Allergy and Clinical Immunology, 102, 558-562
Davis P.J., Smales C.M., James D.C. (2001): How can thermal processing modify the antigenicity of proteins? Allergy, 56: 56–60.
Hernández Marta, Esteve Teresa, Pla Maria (2005): Real-Time Polymerase Chain Reaction Based Assays for Quantitative Detection of Barley, Rice, Sunflower, and Wheat. Journal of Agricultural and Food Chemistry, 53, 7003-7009
Herrero Beatriz, Vieites Juan M., Espiñeira Montserrat (2014): Development of an in-house fast real-time PCR method for detection of fish allergen in foods and comparison with a commercial kit. Food Chemistry, 151, 415-420
Hird H., Lloyd J., Goodier R., Brown J., Reece P. (2003): Detection of peanut using real-time polymerase chain reaction. European Food Research and Technology, 217, 265-268
Navarro E., Serrano-Heras G., Castaño M.J., Solera J. (2015): Real-time PCR detection chemistry. Clinica Chimica Acta, 439, 231-250
Pafundo Simona, Gullì Mariolina, Marmiroli Nelson (2010): Multiplex real-time PCR using SYBR® GreenER™ for the detection of DNA allergens in food. Analytical and Bioanalytical Chemistry, 396, 1831-1839
Palle-Reisch Monika, Hochegger Rupert, Cichna-Markl Margit (2015): Development and validation of a triplex real-time PCR assay for the simultaneous detection of three mustard species and three celery varieties in food. Food Chemistry, 184, 46-56
Poms R.E., Klein C.L., Anklam E. (2004): Methods for allergen analysis in food: a review. Food Additives & Contaminants, 21: 1–31.
Rastogi N. K., Raghavarao K. S. M. S., Balasubramaniam V. M., Niranjan K., Knorr D. (2007): Opportunities and Challenges in High Pressure Processing of Foods. Critical Reviews in Food Science and Nutrition, 47, 69-112
REDMOND ELIZABETH C., GRIFFITH CHRISTOPHER J. (2003): Consumer Food Handling in the Home: A Review of Food Safety Studies. Journal of Food Protection, 66, 130-161
Reed Gudrun H, Kent Jana O, Wittwer Carl T (2007): High-resolution DNA melting analysis for simple and efficient molecular diagnostics. Pharmacogenomics, 8, 597-608
Shin Sang Phil, Ishitani Hiroe, Shirakashi Sho (2016): Development of a multiplex PCR to detect Kudoa spp. and to distinguish Kudoa septempunctata in olive flounder Paralichthys olivaceus. Aquaculture, 464, 37-41
Sicherer Scott H., Sampson Hugh A. (2007): Peanut allergy: Emerging concepts and approaches for an apparent epidemic. Journal of Allergy and Clinical Immunology, 120, 491-503
Sicherer Scott H., Sampson Hugh A. (2014): Food allergy: Epidemiology, pathogenesis, diagnosis, and treatment. Journal of Allergy and Clinical Immunology, 133, 291-307.e5
Wei Shuang, Zhao Hui, Xian Yuyin, Hussain Malik A., Wu Xiyang (2014): Multiplex PCR assays for the detection of Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae with an internal amplification control. Diagnostic Microbiology and Infectious Disease, 79, 115-118
Xu Yi-Gang, Sun Liu-Mei, Wang Yu-Sai, Chen Pei-Pei, Liu Zhong-Mei, Li Yi-Jing, Tang Li-Jie (2017): Simultaneous detection of Vibrio cholerae , Vibrio alginolyticus , Vibrio parahaemolyticus and Vibrio vulnificus in seafood using dual priming oligonucleotide (DPO) system-based multiplex PCR assay. Food Control, 71, 64-70
Zhang Wen-Ju, Cai Qin, Guan Xiao, Chen Qin (2015): Detection of peanut (Arachis hypogaea) allergen by Real-time PCR method with internal amplification control. Food Chemistry, 174, 547-552
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti