Phytochemical and antioxidant screening of some extracts of Juniperus communis L. and Juniperus oxycedrus L.Živić N., Milošević S., Dekić V., Dekić B., Ristić N., Ristić M., Sretić L. (2019): Phytochemical and antioxidant screening of some extracts of Juniperus communis L. and Juniperus oxycedrus L. Czech J. Food Sci., 37: 351-358.
download PDF

The content of phytochemicals, total phenolics, total flavonoids and antioxidant potential of extracts of Juniperus communis L. and Juniperus oxycedrus L. berries were determined. Ethanol, ethyl acetate and chloroform were used for the process of extraction. Phytochemical monitoring was based on already known methods, while in vitro antioxidant activities were done by DPPH assay. Phytochemical screening showed a wide spectrum of phytochemicals. Ethanolic extract of Juniperus communis L. possesses the strongest antioxidant activity (IC50 = 28.55 ± 0.24 µ/ml), as well the higher contents of total phenolics, 189.82 ± 0.27 mg of gallic acid equivalent per g of dried weight extract (mg GAE/g extract DW), and total flavonoids, 42.85 ± 0.13 mg of rutin equivalents per g of dried weight extract (mg RE/g extract DW). The results indicated the potential application of the tested extracts as significant antioxidants.

Abbas K., Babak R., Ali M. (2017): Chemical composition and insecticidal efficacy of Juniperus polycarpus and Juniperus sabinaessential oils against Tribolium confusum (Coleoptera: Tenebrionidae). International Journal of Food Properties, 20: S1221–S1229.
Akhtar S., Ismail T., Fraternale D., Sestili P. (2015): Pomegranate peel and peel extracts: Chemistry and food features. Food Chemistry, 174: 417–425.
Al-Snafi A. (2018): Pharmacological and therapeutic effects of Juniperus Oxycedrus – A Review. Indo American Journal of Pharmaceutical Science, 5: 2198–2205.
Banerjee S., Mukherjee A., Chatterjee T.K. (2012): Evaluation of analgesic activities of methanolic extract of medicinal plant Juniperus communis Linn. International Journal of Pharmacy and Pharmaceutical Science, 4: 547–555.
Braca A., de Tommasi N., Bari L.D., Pizza C., Politi M., Morelli I. (2001): Antioxidant principles from Bauhinia tarapotensis. Journal of Natural Products, 64: 892–895.
Charles D.J. (2013): Antioxidant properties of spices, herbs and other sources. In: Charles D.J.: Juniper. (1st Ed.). New York, Springer: 357–362.
De Marino S., Festa C., Zollo F., Rusolo F., Capone F., Guerriero E.,Costantini S., de Felice V., Iorizzi M. (2014): Phytochemical profile of Juniperus oxycedrus ssp. oxycedrus berries: A new monoterpene glucoside and evaluation of the effects on cancer cell lines. Phytochemistry Letters, 10: 152–159.
Ebrahimzadeh M.A., Khalili M., Dehpour A.A. (2018): Antioxidant activity of ethyl acetate and methanolic extracts of two marine algae, Nannochloropsis oculata and Gracilaria gracilis – an in vitro assay. Brazilian Journal of Pharmaceutical Sciences, 54: e17280. doi: 10.1590/s2175-97902018000117280
Eryilmaz M., Tosun A., Tumen I. (2016): Antimicrobial activity of some species from pinaceae and cupressaceae. Turkish Journal of Pharmaceutical Sciences, 13: 35–40.
Fernandez A., Cock I. (2016): The therapeutic properties of Juniperus communis L.: antioxidant capacity, bacterial growth inhibition, anticancer activity and toxicity. Pharmacognosy Journal, 8: 273–280.
Guedes Mayara N.S., Pio R., Maro Luana A.C., Lage Fabíola F., Abreu Celeste M.P., Saczk Adelir A. (2017): Antioxidant activity and total phenol content of blackberries cultivated in a highland tropical climate. Acta Scientiarum: Agronomy, 39: 43–48.
Hafiza N., Nabil S., Muhammad F, McCullagh J.S.O. (2017): Measurement of total phenolic content and antioxidant activity of aerial parts of medicinal plant Coronopus didymus. Asian Pacific Journal of Tropical Medicine, 10: 792–801.
Harborne J.B. (1984): Methods of Plant Analysis. In: Phytochemical Methods. Springer, Dordrecht: 1–36
Djebaili H., Zellagui A., Gherraf N. (2013): Germacrene-D, a characteristic component of the essential oils from the leaves of Juniperus oxycedrus ssp. macrocarpa (S. et Sm.) ball growing in El Kala, Algeria. Journal of Natural Product and Plant Resources, 3: 40–44.
Höferl M., Stoilova I., Schmidt E., Wanner J., Jirovetz L., Trifonova D., Krastev L., Krastanov A. (2014): Chemical composition and antioxidant properties of Juniper Berry (J. communis L.) Essential oil. Action of the essential oil on the antioxidant protection of Saccharomyces cerevisiae model organism. Antioxidants, 3: 81–98.
Jean N.N., Roland N., Emmanuel T., Alembert T.T., Dieudonné N., Celine H., Sophie L., Joseph T.M. (2018): DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes. Asian Pacific Journal of Tropical Medicine, 11: 280–284.
Kumar R.S., Venkateshwar C., Samuel G., Rao S.G. (2013): Phytochemical screening of some compounds from plant leaf extracts of Holoptelea integrifolia (Planch.) and Celestrus emarginata (Grah.) used by Gondu tribes at Adilabad District, Andhra Pradesh, India. International Journalof Engineering and Science Invention, 2: 65–70.
Lesjak M.M., Beara N.I., Orčić Z.D., Anačkov T.G., Balog J.K., Francišković M.M., Mimica-Dukić M.N. (2011): Juniperus sibirica Burgsdorf as a novel source of antioxidant and anti-inflammatory agents. Food Chemistry, 124: 850–856.
Loizzo M.R., Tundis R., Conforti F., Saab A.M., Statti G.A., Menichini F. (2007): Comparative chemical composition, antioxidant and hypoglycaemic activities of Juniperus oxycedrus ssp. oxycedrus L. berry and wood oils from Lebanon. Food Chemistry, 105: 572–758.
Liu Q., Li D., Wang W., Wang D., Meng X., Wang Y. (2016): Chemical composition and antioxidant activity of essential oils and methanol extracts of different parts from Juniperus rigida Siebold & Zucc. Chemistry & Biodiversity, 13: 1240–1250.
Miceli N., Trovato A., Dugo P., Cacciola F., Donato P., Marino A., Bellinghieri V., La Barbera T.M., Güvenç A., Taviano M.F. (2009): Comparative analysis of flavonoid profile, antioxidant and antimicrobial activity of the berries of Juniperus communis L. var. communis and Juniperus communis L. var. saxatilis Pall. from Turkey. Journal of Agricultural and Food Chemistry, 57: 6570–6577.
Marimuthu P., Wu C.L., Chang H.T., Chang S.T. (2008): Antioxidant activity of the ethanolic extract from the bark of Chamaecyparis obtusa var. formosana. Journal of the Science of Food and Agriculture, 88: 1400–1405.
Orhan N., Orhan I.E., Ergun F. (2011): Insights into cholinesterase inhibitory and antioxidant activities of five Juniperus species. Food and Chemical Toxicology, 49: 2305–2312.
Öztürk M., Tümen İ., Uğur A., Aydoğmuş-Öztürk F., Topçu G. (2011): Evaluation of fruit extracts of six Turkish Juniperus species for their antioxidant, anticholinesterase and antimicrobial activities. Journal of Agricultural and Food Chemistry, 91: 867–876.
Peruč D., Gobin I., Abram M., BroznićD., Svalina T., Štifter S., Stavar M.M., Tićac B. (2018): Antimycobacterial potential of the juniper berry essential oil in tap water. Arhives of Industrial Hygiene and Toxicology, 69: 46–54.
Quy-Diem D., Angkawijaya A., Tran-Nguyen P.L., Huynh L.H., Felycia E.S., Ismadji S., Yi-Hsu J. (2014): Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22: 296–302.
Rajčević N., Janaćković P., Dodoš T., Tešević V., Marin D.P. (2015): Essentialoil variability of Juniperus deltoides R.P.Adams along the east Adriatic coast – how many chemotypes are there? Chemistry & Biodiversity, 12: 82–95.
Shanjani P.S., Mehdi M., Mohsen C. (2016): Composition of the essential oils of Junipers (Juniperus foetidissima and
J. oblonga) from Arasbaran protected area. Journal of Essent Oil Bearing Plants, 19: 1261–1266.
Singleton V.L., Orthofe R., Lamuela-Raventos R.M. (1999): Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299: 152–178.
Spagnuolo C., Moccia S., Russo G.L. (2018): Anti-inflammatory effects of flavonoids in neurodegenerative disorders. European Journal of Medicinal Chemistry, 153: 105–115.
Stoilova I.S., Wanner J., Jirovetz L., Trifonova D., Krastev L., Stoyanova A., Krastanov A. (2014): Chemical composition and antioxidant properties of juniper berry (Juniperus communis L.) essential oil. Bulgarian Journal of Agricultural Science, 20: 227–237.
Šojić B., Tomović V., Jokanović M., Ikonić P., Džinić N., Kocić-Tanackov S., Popović Lj., Tasić T., Savanović J., Živković Šojić N. (2017): Antioxidant activity of Juniperus communis L. essential oil in cooked pork sausages. Czech Journal of Food Science, 35: 189–193.
Taviano M.F., Marino A., Trovato A., Bellinghieri V., LaBarbera M.T., Güvenç A., Hürkul M.M., Pasquale R.D., Miceli N. (2011): Antioxidant and antimicrobial activities of branches extracts of five Juniperus species from Turkey. Pharmaceutical Biology, 49: 1014–1022.
Taviano M.F., Marino A., Trovato A., Bellinghieri V., Melchini A., Dugo P., Cacciola F., Donato P., Mondello L., Güvenç A., De Pasquale R., Miceli N. (2013): Juniperus oxycedrus L. subsp. oxycedrus and Juniperus oxycedrus L. subsp. macrocarpa (Sibth. & Sm.) Ball. ‘berries’ from Turkey: comparative evaluation of phenolic profile, antioxidant, cytotoxic and antimicrobial activities. Food and Chemical Toxicology, 58: 22–29.
Venditti A., Maggi F., Quassinti L., Bramucci M., Lupidi G., Ornano L.,  Ballero M., Sanna C., Bruno M., Rosselli S., Bianco A. (2018): Bioactive constituents of Juniperus turbinata Guss. from La Maddalena Archipelago. Chemistry & Biodiversity, 15: e1800148. doi: 10.1002/cbdv.201800148.
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti